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Dynamic differences in dietary polyunsaturated fatty acid metabolism in sputum of COPD patients and controls.
Biochim Biophys Acta Mol Cell Biol Lipids. 2019 03; 1864(3):224-233.BB

Abstract

INTRODUCTION

Disturbances in onset and resolution of inflammation in chronic obstructive pulmonary disease (COPD) are incompletely understood. Dietary polyunsaturated fatty acids (PUFAs) can be converted into lipid mediators here collectively named oxylipins. These include classical eicosanoids, but also pro-resolving mediators. A balanced production of pro-inflammatory and pro-resolving oxylipins is of importance for adequate inflammatory responses and subsequent return to homeostasis.

OBJECTIVES

Here we investigated if PUFA metabolism is disturbed in COPD patients.

METHODS

Free PUFA and oxylipin levels were measured in induced sputum samples from the Bergen COPD cohort and COPD exacerbation study using liquid chromatography-mass spectrometry. Additionally, effects of whole cigarette smoke on PUFA metabolism in air-liquid interface cultures of primary bronchial epithelial cells were assessed.

RESULTS

Significantly lower levels of free alpha-linolenic acid, linoleic acid and eicosapentaenoic acid (EPA) were detected in sputum from stable COPD patients compared to controls. During acute exacerbation (AE), levels of free arachidonic acid and docosapentaenoic acid were higher than in stable COPD patients. Furthermore, levels of omega-3 EPA- and docosahexaenoic acid-derived oxylipins were lower in sputum from stable COPD patients compared to controls. Cyclooxygenase-2-converted mediators were mostly increased during AE. In vitro studies additionally showed that cigarette smoke exposure may also directly contribute to altered epithelial PUFA metabolism, and indirectly by causing airway epithelial remodelling.

CONCLUSIONS

Our findings show significant differences in PUFA metabolism in COPD patients compared to controls, further changed during AE. Airway epithelial remodelling may contribute to these changes. These findings provide new insight in impaired inflammatory resolution in COPD.

Authors+Show Affiliations

Dept. of Pulmonology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: a.van_der_does@lumc.nl.Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands.Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands.Dept. of Thoracic Medicine, Haukeland University Hospital, Bergen, Norway.Dept. of Thoracic Medicine, Haukeland University Hospital, Bergen, Norway.Dept. of Clinical Science, University of Bergen, Bergen, Norway.Dept. of Thoracic Medicine, Haukeland University Hospital, Bergen, Norway; Dept. of Clinical Science, University of Bergen, Bergen, Norway.Dept. of Pulmonology, Leiden University Medical Center, Leiden, the Netherlands.Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30528790

Citation

van der Does, Anne M., et al. "Dynamic Differences in Dietary Polyunsaturated Fatty Acid Metabolism in Sputum of COPD Patients and Controls." Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids, vol. 1864, no. 3, 2019, pp. 224-233.
van der Does AM, Heijink M, Mayboroda OA, et al. Dynamic differences in dietary polyunsaturated fatty acid metabolism in sputum of COPD patients and controls. Biochim Biophys Acta Mol Cell Biol Lipids. 2019;1864(3):224-233.
van der Does, A. M., Heijink, M., Mayboroda, O. A., Persson, L. J., Aanerud, M., Bakke, P., Eagan, T. M., Hiemstra, P. S., & Giera, M. (2019). Dynamic differences in dietary polyunsaturated fatty acid metabolism in sputum of COPD patients and controls. Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids, 1864(3), 224-233. https://doi.org/10.1016/j.bbalip.2018.11.012
van der Does AM, et al. Dynamic Differences in Dietary Polyunsaturated Fatty Acid Metabolism in Sputum of COPD Patients and Controls. Biochim Biophys Acta Mol Cell Biol Lipids. 2019;1864(3):224-233. PubMed PMID: 30528790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic differences in dietary polyunsaturated fatty acid metabolism in sputum of COPD patients and controls. AU - van der Does,Anne M, AU - Heijink,Marieke, AU - Mayboroda,Oleg A, AU - Persson,Louise J, AU - Aanerud,Marianne, AU - Bakke,Per, AU - Eagan,Tomas M, AU - Hiemstra,Pieter S, AU - Giera,Martin, Y1 - 2018/12/05/ PY - 2018/06/12/received PY - 2018/10/26/revised PY - 2018/11/30/accepted PY - 2018/12/12/pubmed PY - 2019/9/10/medline PY - 2018/12/12/entrez KW - Airway epithelial cells KW - Analysis of sputum KW - COPD KW - Exacerbation KW - Metabolism SP - 224 EP - 233 JF - Biochimica et biophysica acta. Molecular and cell biology of lipids JO - Biochim Biophys Acta Mol Cell Biol Lipids VL - 1864 IS - 3 N2 - INTRODUCTION: Disturbances in onset and resolution of inflammation in chronic obstructive pulmonary disease (COPD) are incompletely understood. Dietary polyunsaturated fatty acids (PUFAs) can be converted into lipid mediators here collectively named oxylipins. These include classical eicosanoids, but also pro-resolving mediators. A balanced production of pro-inflammatory and pro-resolving oxylipins is of importance for adequate inflammatory responses and subsequent return to homeostasis. OBJECTIVES: Here we investigated if PUFA metabolism is disturbed in COPD patients. METHODS: Free PUFA and oxylipin levels were measured in induced sputum samples from the Bergen COPD cohort and COPD exacerbation study using liquid chromatography-mass spectrometry. Additionally, effects of whole cigarette smoke on PUFA metabolism in air-liquid interface cultures of primary bronchial epithelial cells were assessed. RESULTS: Significantly lower levels of free alpha-linolenic acid, linoleic acid and eicosapentaenoic acid (EPA) were detected in sputum from stable COPD patients compared to controls. During acute exacerbation (AE), levels of free arachidonic acid and docosapentaenoic acid were higher than in stable COPD patients. Furthermore, levels of omega-3 EPA- and docosahexaenoic acid-derived oxylipins were lower in sputum from stable COPD patients compared to controls. Cyclooxygenase-2-converted mediators were mostly increased during AE. In vitro studies additionally showed that cigarette smoke exposure may also directly contribute to altered epithelial PUFA metabolism, and indirectly by causing airway epithelial remodelling. CONCLUSIONS: Our findings show significant differences in PUFA metabolism in COPD patients compared to controls, further changed during AE. Airway epithelial remodelling may contribute to these changes. These findings provide new insight in impaired inflammatory resolution in COPD. SN - 1879-2618 UR - https://www.unboundmedicine.com/medline/citation/30528790/Dynamic_differences_in_dietary_polyunsaturated_fatty_acid_metabolism_in_sputum_of_COPD_patients_and_controls_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1388-1981(18)30368-8 DB - PRIME DP - Unbound Medicine ER -