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BDNF mediates the protective effects of scopolamine in reserpine-induced depression-like behaviors via up-regulation of 5-HTT and TPH1.
Psychiatry Res 2019; 271:328-334PR

Abstract

Reserpine treatment in rodents has been shown to induce depression-like behaviors that mimic monoamine dysfunction implicated in the development of depression. Herein, we aimed to demonstrate the antidepressant-like activities of scopolamine, the muscarinic receptor antagonist, in a reserpine-induced mouse model. Mice were injected with 1.5 mg/kg (i.p.) of reserpine for 10 days, and the depression-like state was confirmed via the open field test (OFT) and forced swimming test (FST). Then, the mice were treated with scopolamine (25 µg/kg, i.p.) or saline for 3 days. Ten days of reserpine treatment resulted in a significant decrease in locomotor activity and an increase in immobility time in the OFT and FST, respectively, indicating that ten days of reserpine administration significantly induced depression-like behaviors in mice. However, scopolamine rapidly ameliorated the increase in immobility time in the FST and had no effect on locomotor activity in the OFT. In addition, the reserpine-induced decreases in serotonin transporter (5-HTT), brain-derived neurotrophic factor (BDNF) and tryptophan hydroxylase 1 (TPH1) in mouse hippocampus and prefrontal cortex (PFC) were significantly reversed by scopolamine. Our study provides evidence that scopolamine rapidly attenuates reserpine-induced depression in mice partially by regulating 5-HTT, BDNF and TPH1 in the hippocampus and PFC of mice.

Authors+Show Affiliations

Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Department of Physiology and Pharmacology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China.Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Department of Physiology and Pharmacology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China.Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Department of Physiology and Pharmacology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China.Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Department of Physiology and Pharmacology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China.Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China. Electronic address: wyzhouds@sina.com.Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China. Electronic address: chenzhongming@hotmail.com.Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, China; Ningbo Key Laboratory of Behavioral Neuroscience, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China; Department of Physiology and Pharmacology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, Zhejiang 315211, China. Electronic address: wangchuang@nbu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30529315

Citation

Yu, Hanjie, et al. "BDNF Mediates the Protective Effects of Scopolamine in Reserpine-induced Depression-like Behaviors Via Up-regulation of 5-HTT and TPH1." Psychiatry Research, vol. 271, 2019, pp. 328-334.
Yu H, Lv D, Shen M, et al. BDNF mediates the protective effects of scopolamine in reserpine-induced depression-like behaviors via up-regulation of 5-HTT and TPH1. Psychiatry Res. 2019;271:328-334.
Yu, H., Lv, D., Shen, M., Zhang, Y., Zhou, D., Chen, Z., & Wang, C. (2019). BDNF mediates the protective effects of scopolamine in reserpine-induced depression-like behaviors via up-regulation of 5-HTT and TPH1. Psychiatry Research, 271, pp. 328-334. doi:10.1016/j.psychres.2018.12.015.
Yu H, et al. BDNF Mediates the Protective Effects of Scopolamine in Reserpine-induced Depression-like Behaviors Via Up-regulation of 5-HTT and TPH1. Psychiatry Res. 2019;271:328-334. PubMed PMID: 30529315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - BDNF mediates the protective effects of scopolamine in reserpine-induced depression-like behaviors via up-regulation of 5-HTT and TPH1. AU - Yu,Hanjie, AU - Lv,Dan, AU - Shen,Mengxin, AU - Zhang,Yanhua, AU - Zhou,Dongsheng, AU - Chen,Zhongming, AU - Wang,Chuang, Y1 - 2018/12/03/ PY - 2018/08/30/received PY - 2018/11/18/revised PY - 2018/12/03/accepted PY - 2018/12/12/pubmed PY - 2019/4/16/medline PY - 2018/12/12/entrez KW - Brain-derived neurotrophic factor (BDNF) KW - Depression KW - Scopolamine KW - Serotonin transporter (5-HTT) KW - Tryptophan hydroxylase 1 (TPH1) SP - 328 EP - 334 JF - Psychiatry research JO - Psychiatry Res VL - 271 N2 - Reserpine treatment in rodents has been shown to induce depression-like behaviors that mimic monoamine dysfunction implicated in the development of depression. Herein, we aimed to demonstrate the antidepressant-like activities of scopolamine, the muscarinic receptor antagonist, in a reserpine-induced mouse model. Mice were injected with 1.5 mg/kg (i.p.) of reserpine for 10 days, and the depression-like state was confirmed via the open field test (OFT) and forced swimming test (FST). Then, the mice were treated with scopolamine (25 µg/kg, i.p.) or saline for 3 days. Ten days of reserpine treatment resulted in a significant decrease in locomotor activity and an increase in immobility time in the OFT and FST, respectively, indicating that ten days of reserpine administration significantly induced depression-like behaviors in mice. However, scopolamine rapidly ameliorated the increase in immobility time in the FST and had no effect on locomotor activity in the OFT. In addition, the reserpine-induced decreases in serotonin transporter (5-HTT), brain-derived neurotrophic factor (BDNF) and tryptophan hydroxylase 1 (TPH1) in mouse hippocampus and prefrontal cortex (PFC) were significantly reversed by scopolamine. Our study provides evidence that scopolamine rapidly attenuates reserpine-induced depression in mice partially by regulating 5-HTT, BDNF and TPH1 in the hippocampus and PFC of mice. SN - 1872-7123 UR - https://www.unboundmedicine.com/medline/citation/30529315/BDNF_mediates_the_protective_effects_of_scopolamine_in_reserpine_induced_depression_like_behaviors_via_up_regulation_of_5_HTT_and_TPH1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-1781(18)31617-2 DB - PRIME DP - Unbound Medicine ER -