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STIM1 activation of Orai1.
Cell Calcium. 2019 01; 77:29-38.CC

Abstract

A primary calcium (Ca2+) entry pathway into non-excitable cells is through the store-operated Ca2+ release activated Ca2+ (CRAC) channel. Ca2+ entry into cells is responsible for the initiation of diverse signalling cascades that affect essential cellular processes like gene regulation, cell growth and death, secretion and gene transcription. Upon depletion of intracellular Ca2+ stores within the endoplasmic reticulum (ER), the CRAC channel opens to refill depleted stores. The two key limiting molecular players of the CRAC channel are the stromal interaction molecule (STIM1) embedded in the ER-membrane and Orai1, residing in the plasma membrane (PM), respectively. Together, they form a highly Ca2+ selective ion channel complex. STIM1 senses the Ca2+ content of the ER and confers Ca2+ store-depletion into the opening of Orai1 channels in the PM for triggering Ca2+-dependent gene transcription, T-cell activation or mast cell degranulation. The interplay of Orai and STIM proteins in the CRAC channel signalling cascade has been the main focus of research for more than twelve years. This chapter focuses on current knowledge and main experimental advances in the understanding of Orai1 activation by STIM1, thereby portraying key mechanistic steps in the CRAC channel signalling cascade.

Authors+Show Affiliations

Institute of Biophysics, Johannes Kepler University Linz, A-4020, Linz, Austria.Institute of Biophysics, Johannes Kepler University Linz, A-4020, Linz, Austria. Electronic address: christoph.romanin@jku.at.Institute of Biophysics, Johannes Kepler University Linz, A-4020, Linz, Austria. Electronic address: irene.frischauf@jku.at.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

30530091

Citation

Lunz, Victoria, et al. "STIM1 Activation of Orai1." Cell Calcium, vol. 77, 2019, pp. 29-38.
Lunz V, Romanin C, Frischauf I. STIM1 activation of Orai1. Cell Calcium. 2019;77:29-38.
Lunz, V., Romanin, C., & Frischauf, I. (2019). STIM1 activation of Orai1. Cell Calcium, 77, 29-38. https://doi.org/10.1016/j.ceca.2018.11.009
Lunz V, Romanin C, Frischauf I. STIM1 Activation of Orai1. Cell Calcium. 2019;77:29-38. PubMed PMID: 30530091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - STIM1 activation of Orai1. AU - Lunz,Victoria, AU - Romanin,Christoph, AU - Frischauf,Irene, Y1 - 2018/11/30/ PY - 2018/09/07/received PY - 2018/11/20/revised PY - 2018/11/28/accepted PY - 2018/12/12/pubmed PY - 2020/3/13/medline PY - 2018/12/12/entrez KW - CRAC KW - Ca(2+) signaling KW - Orai KW - STIM SP - 29 EP - 38 JF - Cell calcium JO - Cell Calcium VL - 77 N2 - A primary calcium (Ca2+) entry pathway into non-excitable cells is through the store-operated Ca2+ release activated Ca2+ (CRAC) channel. Ca2+ entry into cells is responsible for the initiation of diverse signalling cascades that affect essential cellular processes like gene regulation, cell growth and death, secretion and gene transcription. Upon depletion of intracellular Ca2+ stores within the endoplasmic reticulum (ER), the CRAC channel opens to refill depleted stores. The two key limiting molecular players of the CRAC channel are the stromal interaction molecule (STIM1) embedded in the ER-membrane and Orai1, residing in the plasma membrane (PM), respectively. Together, they form a highly Ca2+ selective ion channel complex. STIM1 senses the Ca2+ content of the ER and confers Ca2+ store-depletion into the opening of Orai1 channels in the PM for triggering Ca2+-dependent gene transcription, T-cell activation or mast cell degranulation. The interplay of Orai and STIM proteins in the CRAC channel signalling cascade has been the main focus of research for more than twelve years. This chapter focuses on current knowledge and main experimental advances in the understanding of Orai1 activation by STIM1, thereby portraying key mechanistic steps in the CRAC channel signalling cascade. SN - 1532-1991 UR - https://www.unboundmedicine.com/medline/citation/30530091/STIM1_activation_of_Orai1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4160(18)30166-0 DB - PRIME DP - Unbound Medicine ER -