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Ketogenic diet increases mitochondria volume in the liver and skeletal muscle without altering oxidative stress markers in rats.
Heliyon 2018; 4(11):e00975H

Abstract

Ketogenic diets (KD) consist of high fat, moderate protein and low carbohydrates. Studies have suggested that KD may influence oxidative stress by affecting mitochondrial quantity and/or quality, and perhaps lengthen lifespan. Therefore, we determined the effects of KD on multi-organ mitochondria volume and oxidative stress markers in rats. Ten month-old male Fisher 344 rats (n = 8 per group) were provided with one of two isocaloric diets: standard chow (SC) or KD. Rats were euthanized if: a) vitality scores exceeded a score of 16, b) rapid weight loss, or c) veterinarian deemed euthanasia necessary. The median lifespan of rats was higher in KD (762 days) compared to SC (624 days). Citrate synthase activity (i.e. estimate of mitochondria volume) was higher in the liver (p = 0.034) and gastrocnemius (p = 0.041) of KD compared to SC. Liver superoxide dismutase 1 and catalase antioxidant protein levels were higher in KD, albeit not significant (p = 0.094 and p = 0.062, respectively). No significant differences in protein levels of other antioxidants or markers of lipid and protein oxidative damage were observed in either the gastrocnemius, liver, or brain. In summary, KD increased mitochondria volume in liver and gastrocnemius and median lifespan in rats. Additionally, our data show that the increase in mitochondrial volume occurred without changes in oxidative damage or antioxidant protein levels in the gastrocnemius, liver, or brain.

Authors+Show Affiliations

School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.School of Kinesiology, Auburn University, Auburn, AL, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30533548

Citation

Parry, Hailey A., et al. "Ketogenic Diet Increases Mitochondria Volume in the Liver and Skeletal Muscle Without Altering Oxidative Stress Markers in Rats." Heliyon, vol. 4, no. 11, 2018, pp. e00975.
Parry HA, Kephart WC, Mumford PW, et al. Ketogenic diet increases mitochondria volume in the liver and skeletal muscle without altering oxidative stress markers in rats. Heliyon. 2018;4(11):e00975.
Parry, H. A., Kephart, W. C., Mumford, P. W., Romero, M. A., Mobley, C. B., Zhang, Y., ... Kavazis, A. N. (2018). Ketogenic diet increases mitochondria volume in the liver and skeletal muscle without altering oxidative stress markers in rats. Heliyon, 4(11), pp. e00975. doi:10.1016/j.heliyon.2018.e00975.
Parry HA, et al. Ketogenic Diet Increases Mitochondria Volume in the Liver and Skeletal Muscle Without Altering Oxidative Stress Markers in Rats. Heliyon. 2018;4(11):e00975. PubMed PMID: 30533548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ketogenic diet increases mitochondria volume in the liver and skeletal muscle without altering oxidative stress markers in rats. AU - Parry,Hailey A, AU - Kephart,Wesley C, AU - Mumford,Petey W, AU - Romero,Matthew A, AU - Mobley,C Brooks, AU - Zhang,Yufeng, AU - Roberts,Michael D, AU - Kavazis,Andreas N, Y1 - 2018/11/24/ PY - 2018/05/15/received PY - 2018/08/22/revised PY - 2018/11/21/accepted PY - 2018/12/12/entrez PY - 2018/12/12/pubmed PY - 2018/12/12/medline KW - Metabolism KW - Nutrition KW - Physiology SP - e00975 EP - e00975 JF - Heliyon JO - Heliyon VL - 4 IS - 11 N2 - Ketogenic diets (KD) consist of high fat, moderate protein and low carbohydrates. Studies have suggested that KD may influence oxidative stress by affecting mitochondrial quantity and/or quality, and perhaps lengthen lifespan. Therefore, we determined the effects of KD on multi-organ mitochondria volume and oxidative stress markers in rats. Ten month-old male Fisher 344 rats (n = 8 per group) were provided with one of two isocaloric diets: standard chow (SC) or KD. Rats were euthanized if: a) vitality scores exceeded a score of 16, b) rapid weight loss, or c) veterinarian deemed euthanasia necessary. The median lifespan of rats was higher in KD (762 days) compared to SC (624 days). Citrate synthase activity (i.e. estimate of mitochondria volume) was higher in the liver (p = 0.034) and gastrocnemius (p = 0.041) of KD compared to SC. Liver superoxide dismutase 1 and catalase antioxidant protein levels were higher in KD, albeit not significant (p = 0.094 and p = 0.062, respectively). No significant differences in protein levels of other antioxidants or markers of lipid and protein oxidative damage were observed in either the gastrocnemius, liver, or brain. In summary, KD increased mitochondria volume in liver and gastrocnemius and median lifespan in rats. Additionally, our data show that the increase in mitochondrial volume occurred without changes in oxidative damage or antioxidant protein levels in the gastrocnemius, liver, or brain. SN - 2405-8440 UR - https://www.unboundmedicine.com/medline/citation/30533548/Ketogenic_diet_increases_mitochondria_volume_in_the_liver_and_skeletal_muscle_without_altering_oxidative_stress_markers_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2405-8440(18)32106-6 DB - PRIME DP - Unbound Medicine ER -