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Inhibiting lncRNA ROR suppresses growth, migration and angiogenesis in microvascular endothelial cells by up-regulating miR-26.
Eur Rev Med Pharmacol Sci. 2018 11; 22(22):7985-7993.ER

Abstract

OBJECTIVE

Lower extremity arteriosclerosis is one of leading causes of death worldwide. Arteriosclerosis is closely related to microvascular endothelial cells. This study was aimed to explore the role of long non-coding RNA ROR in regulations of growth, migration, and angiogenesis of microvascular endothelial cells.

MATERIALS AND METHODS

Angiogenesis was determined by the number of tube-like cells on a matrigel extracellular matrix. Cell viability, apoptosis, and migration were determined by CCK-8 assay, PI/FITC-Annexin V staining method, and transwell assay, respectively. Relative RNA expression of ROR, miR-26, and angiogenesis-associated genes were analyzed by qRT-PCR. The protein expression of apoptosis- and angiogenesis-associated genes, as well as main factors in NF-κB and JAK1/STAT3 pathways, were analyzed by Western blot.

RESULTS

LncRNA ROR silence inhibited viability, migration, and angiogenesis of HMEC-1 cells but promoted apoptosis of them. miR-26 expression was promoted after knocking down ROR expression. miR-26 overexpression enhanced the inhibitory effects of ROR silence on growth, migration, and angiogenesis in HMEC-1 cells, whereas, miR-26 silence impaired the effects of ROR silence. Finally, we found that NF-κB and JAK1/STAT3 signaling pathways were inhibited by ROR down-regulation. Similarly, miR-26 overexpression enhanced the inhibitory effect of ROR down-regulation on the pathways and miR-26 inhibition abrogated it.

CONCLUSIONS

Down-regulating lncRNA ROR inhibited growth, migration and angiogenesis of microvascular endothelial cells possibly through up-regulation of miR-26. During this process, the activations of NF-κB and JAK1/STAT3 pathways were inhibited after interaction of ROR and miR-26.

Authors+Show Affiliations

Department of Vascular Surgery, Jining No.1 People's Hospital, Jining, Shandong Province, China. wxu131@sina.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30536347

Citation

Qin, W-W, et al. "Inhibiting lncRNA ROR Suppresses Growth, Migration and Angiogenesis in Microvascular Endothelial Cells By Up-regulating MiR-26." European Review for Medical and Pharmacological Sciences, vol. 22, no. 22, 2018, pp. 7985-7993.
Qin WW, Xin ZL, Wang HQ, et al. Inhibiting lncRNA ROR suppresses growth, migration and angiogenesis in microvascular endothelial cells by up-regulating miR-26. Eur Rev Med Pharmacol Sci. 2018;22(22):7985-7993.
Qin, W. W., Xin, Z. L., Wang, H. Q., Wang, K. P., Li, X. Y., & Wang, X. (2018). Inhibiting lncRNA ROR suppresses growth, migration and angiogenesis in microvascular endothelial cells by up-regulating miR-26. European Review for Medical and Pharmacological Sciences, 22(22), 7985-7993. https://doi.org/10.26355/eurrev_201811_16427
Qin WW, et al. Inhibiting lncRNA ROR Suppresses Growth, Migration and Angiogenesis in Microvascular Endothelial Cells By Up-regulating MiR-26. Eur Rev Med Pharmacol Sci. 2018;22(22):7985-7993. PubMed PMID: 30536347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibiting lncRNA ROR suppresses growth, migration and angiogenesis in microvascular endothelial cells by up-regulating miR-26. AU - Qin,W-W, AU - Xin,Z-L, AU - Wang,H-Q, AU - Wang,K-P, AU - Li,X-Y, AU - Wang,X, PY - 2018/12/12/entrez PY - 2018/12/12/pubmed PY - 2019/11/15/medline SP - 7985 EP - 7993 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 22 N2 - OBJECTIVE: Lower extremity arteriosclerosis is one of leading causes of death worldwide. Arteriosclerosis is closely related to microvascular endothelial cells. This study was aimed to explore the role of long non-coding RNA ROR in regulations of growth, migration, and angiogenesis of microvascular endothelial cells. MATERIALS AND METHODS: Angiogenesis was determined by the number of tube-like cells on a matrigel extracellular matrix. Cell viability, apoptosis, and migration were determined by CCK-8 assay, PI/FITC-Annexin V staining method, and transwell assay, respectively. Relative RNA expression of ROR, miR-26, and angiogenesis-associated genes were analyzed by qRT-PCR. The protein expression of apoptosis- and angiogenesis-associated genes, as well as main factors in NF-κB and JAK1/STAT3 pathways, were analyzed by Western blot. RESULTS: LncRNA ROR silence inhibited viability, migration, and angiogenesis of HMEC-1 cells but promoted apoptosis of them. miR-26 expression was promoted after knocking down ROR expression. miR-26 overexpression enhanced the inhibitory effects of ROR silence on growth, migration, and angiogenesis in HMEC-1 cells, whereas, miR-26 silence impaired the effects of ROR silence. Finally, we found that NF-κB and JAK1/STAT3 signaling pathways were inhibited by ROR down-regulation. Similarly, miR-26 overexpression enhanced the inhibitory effect of ROR down-regulation on the pathways and miR-26 inhibition abrogated it. CONCLUSIONS: Down-regulating lncRNA ROR inhibited growth, migration and angiogenesis of microvascular endothelial cells possibly through up-regulation of miR-26. During this process, the activations of NF-κB and JAK1/STAT3 pathways were inhibited after interaction of ROR and miR-26. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30536347/Inhibiting_lncRNA_ROR_suppresses_growth_migration_and_angiogenesis_in_microvascular_endothelial_cells_by_up_regulating_miR_26_ L2 - https://www.europeanreview.org/article/16427 DB - PRIME DP - Unbound Medicine ER -