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Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs.
Cell Biol Int. 2019 Feb; 43(2):158-166.CB

Abstract

Long noncoding RNAs (lncRNAs) have been found to play important roles in nearly all biological processes. However, the functions of the majority of LncRNAs are not fully clear. Here we evaluated the function of lncRNA OGFRP1, which has not been previously annotated, in human coronary artery endothelial cells (HCAECs). First, we knocked down lncRNA OGFRP1 in HCAECs by using siRNA transfection. qRT-PCR results indicated that siRNA1 and siRNA3 both had potent interference efficiencies. Next, by using CCK8 assay and clone formation assay, we found that siRNA3 transfection induced growth inhibition in HCAECs. Cell migration and invasion were also found to be inhibited in OGFRP1 silenced cells. Moreover, siRNA1 transfection further verified the inhibitory effects of lncRNA OGFRP1 knockdown on the proliferation, migration and invasion of HCAECs. Flow cytometry detection demonstrated that OGFRP1 knockdown induced cell cycle arrest and apoptosis. Western blot assay indicated that p70S6K and CyclinD1 were down-regulated by knockdown of OGFRP1. The intrinsic apoptosis pathway was activated in lncRNA OGFRP1 silenced cells, including increased Bax and Active-caspase 3 and decreased Bcl2. The expression of autophagy markers LC3 and Beclin1 was increased and p62 decreased, all of which indicated that cell autophagy was promoted by down-regulation of lncRNA OGFRP1. Mechanistic studies showed that lncRNA OGFRP1 inhibited the AKT/mTOR signaling pathway, including increasing phosphorylation level of AKT, mTOR and GSK3β. In conclusion, we find that down-regulation of lncRNA induces autophagy and inhibited the proliferation, migration and invasion by AKT/mTOR signaling pathway in HCAECs.

Authors+Show Affiliations

Department of Clinical Laboratory, Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, 264000, P. R. China.Department of Cardiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, 264100, P. R. China.Department of Clinical Laboratory, Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, 264000, P. R. China.Department of Clinical Laboratory, Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, 264000, P. R. China.Department of Cardiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, 264100, P. R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30536541

Citation

Zhang, Xiaolu, et al. "Down-regulation of lncRNA OGFRP1 Induces Autophagy and Growth Inhibition By AKT/mTOR Signaling Pathway in HCAECs." Cell Biology International, vol. 43, no. 2, 2019, pp. 158-166.
Zhang X, Liu J, Gu Y, et al. Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs. Cell Biol Int. 2019;43(2):158-166.
Zhang, X., Liu, J., Gu, Y., Sun, C., & Qu, F. (2019). Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs. Cell Biology International, 43(2), 158-166. https://doi.org/10.1002/cbin.11081
Zhang X, et al. Down-regulation of lncRNA OGFRP1 Induces Autophagy and Growth Inhibition By AKT/mTOR Signaling Pathway in HCAECs. Cell Biol Int. 2019;43(2):158-166. PubMed PMID: 30536541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs. AU - Zhang,Xiaolu, AU - Liu,Jing, AU - Gu,Yufeng, AU - Sun,Chengming, AU - Qu,Fuzheng, Y1 - 2019/01/08/ PY - 2018/06/13/received PY - 2018/12/04/accepted PY - 2018/12/12/pubmed PY - 2019/5/1/medline PY - 2018/12/12/entrez KW - PI3K/AKT KW - apoptosis KW - autophagy KW - cell cycle KW - lncRNA OGFRP1 KW - proliferation SP - 158 EP - 166 JF - Cell biology international JO - Cell Biol. Int. VL - 43 IS - 2 N2 - Long noncoding RNAs (lncRNAs) have been found to play important roles in nearly all biological processes. However, the functions of the majority of LncRNAs are not fully clear. Here we evaluated the function of lncRNA OGFRP1, which has not been previously annotated, in human coronary artery endothelial cells (HCAECs). First, we knocked down lncRNA OGFRP1 in HCAECs by using siRNA transfection. qRT-PCR results indicated that siRNA1 and siRNA3 both had potent interference efficiencies. Next, by using CCK8 assay and clone formation assay, we found that siRNA3 transfection induced growth inhibition in HCAECs. Cell migration and invasion were also found to be inhibited in OGFRP1 silenced cells. Moreover, siRNA1 transfection further verified the inhibitory effects of lncRNA OGFRP1 knockdown on the proliferation, migration and invasion of HCAECs. Flow cytometry detection demonstrated that OGFRP1 knockdown induced cell cycle arrest and apoptosis. Western blot assay indicated that p70S6K and CyclinD1 were down-regulated by knockdown of OGFRP1. The intrinsic apoptosis pathway was activated in lncRNA OGFRP1 silenced cells, including increased Bax and Active-caspase 3 and decreased Bcl2. The expression of autophagy markers LC3 and Beclin1 was increased and p62 decreased, all of which indicated that cell autophagy was promoted by down-regulation of lncRNA OGFRP1. Mechanistic studies showed that lncRNA OGFRP1 inhibited the AKT/mTOR signaling pathway, including increasing phosphorylation level of AKT, mTOR and GSK3β. In conclusion, we find that down-regulation of lncRNA induces autophagy and inhibited the proliferation, migration and invasion by AKT/mTOR signaling pathway in HCAECs. SN - 1095-8355 UR - https://www.unboundmedicine.com/medline/citation/30536541/Down_regulation_of_lncRNA_OGFRP1_induces_autophagy_and_growth_inhibition_by_AKT/mTOR_signaling_pathway_in_HCAECs_ L2 - https://doi.org/10.1002/cbin.11081 DB - PRIME DP - Unbound Medicine ER -