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EFFECTS OF RENAL DYSFUNCTION ON HEALING OF COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY IN WISTAR RATS.
Arq Bras Cir Dig. 2018 Dec 06; 31(4):e1398.AB

Abstract

BACKGROUND

Chronic kidney disease affects more than 500 million people worldwide. In this context, the uremic toxins present are related to worsening in tissue healing.

AIM

Evaluate on healing of colonic anastomosis in uremic rats, serum and anatomopathological indicators, which may be related to the change tissue repair process.

METHODS

Twenty Wistar rats, were randomly separated into two groups. In the sham group they were submitted to 5/6 nephrectomy simulation in left kidney, simulation right nephrectomy, median laparotomy, colotomy and colorraphy. In the uremia group, they were submitted to 5/6 nephrectomy of the left kidney, total nephrectomy of the right kidney and median laparotomy, colotomy and colorraphy. Were collected for serum urea, creatinine and CRP dosages and the colonic segments were studied for evaluation of granulation tissue, collagen maturation, microvascular and myofibroblasts density, and cell viability. Through histochemical processing, microvascular density was evaluated by anti-CD34 monoclonal antibody marking, cell viability by cell proliferation nuclear antigen screening and myofibroblasts density with monoclonal anti-α-actin antibody. Computerized histometry was used for evaluations of collagens type I and III by the coloration of picrosirius.

RESULTS

The group submitted to nephrectomy 5/6, compared to the sham group, show urea increase (p<0.0000) and higher C reactive protein (p=0.0142). Decrease of granulation tissue formation (border reepithelialization p=0,0196, angiofibroblast proliferation p=0.0379), mean collagen I (p=0,0009) and collagen III (p=0,016), microvascular density (p=0,0074), cell proliferation nuclear antigen (p<0,0000) and myofibroblasts (p<0,0001).

CONCLUSION

The uremia induced by nephrectomy 5/6 model establishes negative impact in the colonic wound healing.

Authors+Show Affiliations

Experimental Research Laboratory, Maternity Hospital Angelina Caron, Campina Grande do Sul, PR. Program of Post-Graduation in Surgical Clinic of the Federal University of Paraná, Curitiba, PR, Brazil.Experimental Research Laboratory, Maternity Hospital Angelina Caron, Campina Grande do Sul, PR. Program of Post-Graduation in Surgical Clinic of the Federal University of Paraná, Curitiba, PR, Brazil.Experimental Research Laboratory, Maternity Hospital Angelina Caron, Campina Grande do Sul, PR.Program of Post-Graduation in Surgical Clinic of the Federal University of Paraná, Curitiba, PR, Brazil.

Pub Type(s)

Journal Article

Language

eng por

PubMed ID

30539973

Citation

Silva, Carlos Eduardo da, et al. "EFFECTS of RENAL DYSFUNCTION ON HEALING of COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY in WISTAR RATS." Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery, vol. 31, no. 4, 2018, pp. e1398.
Silva CED, Repka JCD, Souza CJF, et al. EFFECTS OF RENAL DYSFUNCTION ON HEALING OF COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY IN WISTAR RATS. Arq Bras Cir Dig. 2018;31(4):e1398.
Silva, C. E. D., Repka, J. C. D., Souza, C. J. F., & Matias, J. E. F. (2018). EFFECTS OF RENAL DYSFUNCTION ON HEALING OF COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY IN WISTAR RATS. Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery, 31(4), e1398. https://doi.org/10.1590/0102-672020180001e1398
Silva CED, et al. EFFECTS of RENAL DYSFUNCTION ON HEALING of COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY in WISTAR RATS. Arq Bras Cir Dig. 2018 Dec 6;31(4):e1398. PubMed PMID: 30539973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EFFECTS OF RENAL DYSFUNCTION ON HEALING OF COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY IN WISTAR RATS. AU - Silva,Carlos Eduardo da, AU - Repka,João Carlos Domingues, AU - Souza,Carlos José Franco de, AU - Matias,Jorge Eduardo Fouto, Y1 - 2018/12/06/ PY - 2018/06/19/received PY - 2018/09/05/accepted PY - 2018/12/13/entrez PY - 2018/12/13/pubmed PY - 2019/5/6/medline SP - e1398 EP - e1398 JF - Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery JO - Arq Bras Cir Dig VL - 31 IS - 4 N2 - BACKGROUND: Chronic kidney disease affects more than 500 million people worldwide. In this context, the uremic toxins present are related to worsening in tissue healing. AIM: Evaluate on healing of colonic anastomosis in uremic rats, serum and anatomopathological indicators, which may be related to the change tissue repair process. METHODS: Twenty Wistar rats, were randomly separated into two groups. In the sham group they were submitted to 5/6 nephrectomy simulation in left kidney, simulation right nephrectomy, median laparotomy, colotomy and colorraphy. In the uremia group, they were submitted to 5/6 nephrectomy of the left kidney, total nephrectomy of the right kidney and median laparotomy, colotomy and colorraphy. Were collected for serum urea, creatinine and CRP dosages and the colonic segments were studied for evaluation of granulation tissue, collagen maturation, microvascular and myofibroblasts density, and cell viability. Through histochemical processing, microvascular density was evaluated by anti-CD34 monoclonal antibody marking, cell viability by cell proliferation nuclear antigen screening and myofibroblasts density with monoclonal anti-α-actin antibody. Computerized histometry was used for evaluations of collagens type I and III by the coloration of picrosirius. RESULTS: The group submitted to nephrectomy 5/6, compared to the sham group, show urea increase (p<0.0000) and higher C reactive protein (p=0.0142). Decrease of granulation tissue formation (border reepithelialization p=0,0196, angiofibroblast proliferation p=0.0379), mean collagen I (p=0,0009) and collagen III (p=0,016), microvascular density (p=0,0074), cell proliferation nuclear antigen (p<0,0000) and myofibroblasts (p<0,0001). CONCLUSION: The uremia induced by nephrectomy 5/6 model establishes negative impact in the colonic wound healing. SN - 2317-6326 UR - https://www.unboundmedicine.com/medline/citation/30539973/EFFECTS_OF_RENAL_DYSFUNCTION_ON_HEALING_OF_COLONIC_ANASTOMOSIS:_EXPERIMENTAL_STUDY_IN_WISTAR_RATS_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0102-67202018000400301&amp;lng=en&amp;nrm=iso&amp;tlng=en DB - PRIME DP - Unbound Medicine ER -