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Dual-Channel Photoelectrochemical Ratiometric Aptasensor with up-Converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device.
Anal Chem. 2019 01 15; 91(2):1260-1268.AC

Abstract

A near-infrared light-activated ratiometric photoelectrochemical aptasensor was fabricated for detection of carcinoembryonic antigen (CEA) coupling with upconversion nanoparticles (UCNPs)-semiconductor nanocrystals-based spatial-resolved technique on a homemade 3D printing device in which a self-regulating integrated electrode was designed for dual signal readout. The as-prepared NaYF4:Yb,Er UCNPs@CdTe nanocrystals were initially assembled on two adjacent photoelectrodes, then CEA aptamer 1 (A1) and capture DNA (CA) were modified onto two working photoelectrodes (WP1 and WP2) through covalent binding, respectively, and then gold nanoparticle-labeled CEA aptamer 2 (Au NP-A2) was immobilized on the surface of functional WP2 for the formation of double-stranded DNA. Upon target CEA introduction, the various concentrations of CEA were captured on the WP1, whereas the binding of the CEA with Au NP-A2 could be released from the WP2 thanks to the highly affinity of CEA toward A2. The dual signal readout with the "signal-off" of WP1 and "signal-on" of WP2 were employed for the spatial-resolved PEC (SR-PEC) strategy to detect CEA as an analytical model. Combining NaYF4:Yb,Er UCNPs@CdTe nanocrystals with spatial-resolved model on 3D printing device, the PEC ratiometric aptasensor based on steric hindrance effect and exciton-plasmon interactions (EPI) exhibited a linear range from 10.0 pg mL-1 to 5.0 ng mL-1 with a limit of detection of 4.8 pg mL-1 under 980 nm illumination. The SR-PEC ratiometric strategy showed acceptable stability and reproducibility with a superior anti-interference ability. This approach can provide the guidance for the design of ratiometric, multiplexed, and point-of-care biosensors.

Authors+Show Affiliations

MOE Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.MOE Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025 , People's Republic of China.MOE Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30543292

Citation

Qiu, Zhenli, et al. "Dual-Channel Photoelectrochemical Ratiometric Aptasensor With up-Converting Nanocrystals Using Spatial-Resolved Technique On Homemade 3D Printed Device." Analytical Chemistry, vol. 91, no. 2, 2019, pp. 1260-1268.
Qiu Z, Shu J, Liu J, et al. Dual-Channel Photoelectrochemical Ratiometric Aptasensor with up-Converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device. Anal Chem. 2019;91(2):1260-1268.
Qiu, Z., Shu, J., Liu, J., & Tang, D. (2019). Dual-Channel Photoelectrochemical Ratiometric Aptasensor with up-Converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device. Analytical Chemistry, 91(2), 1260-1268. https://doi.org/10.1021/acs.analchem.8b05455
Qiu Z, et al. Dual-Channel Photoelectrochemical Ratiometric Aptasensor With up-Converting Nanocrystals Using Spatial-Resolved Technique On Homemade 3D Printed Device. Anal Chem. 2019 01 15;91(2):1260-1268. PubMed PMID: 30543292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual-Channel Photoelectrochemical Ratiometric Aptasensor with up-Converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device. AU - Qiu,Zhenli, AU - Shu,Jian, AU - Liu,Jingfeng, AU - Tang,Dianping, Y1 - 2018/12/21/ PY - 2018/12/14/pubmed PY - 2020/7/10/medline PY - 2018/12/14/entrez SP - 1260 EP - 1268 JF - Analytical chemistry JO - Anal Chem VL - 91 IS - 2 N2 - A near-infrared light-activated ratiometric photoelectrochemical aptasensor was fabricated for detection of carcinoembryonic antigen (CEA) coupling with upconversion nanoparticles (UCNPs)-semiconductor nanocrystals-based spatial-resolved technique on a homemade 3D printing device in which a self-regulating integrated electrode was designed for dual signal readout. The as-prepared NaYF4:Yb,Er UCNPs@CdTe nanocrystals were initially assembled on two adjacent photoelectrodes, then CEA aptamer 1 (A1) and capture DNA (CA) were modified onto two working photoelectrodes (WP1 and WP2) through covalent binding, respectively, and then gold nanoparticle-labeled CEA aptamer 2 (Au NP-A2) was immobilized on the surface of functional WP2 for the formation of double-stranded DNA. Upon target CEA introduction, the various concentrations of CEA were captured on the WP1, whereas the binding of the CEA with Au NP-A2 could be released from the WP2 thanks to the highly affinity of CEA toward A2. The dual signal readout with the "signal-off" of WP1 and "signal-on" of WP2 were employed for the spatial-resolved PEC (SR-PEC) strategy to detect CEA as an analytical model. Combining NaYF4:Yb,Er UCNPs@CdTe nanocrystals with spatial-resolved model on 3D printing device, the PEC ratiometric aptasensor based on steric hindrance effect and exciton-plasmon interactions (EPI) exhibited a linear range from 10.0 pg mL-1 to 5.0 ng mL-1 with a limit of detection of 4.8 pg mL-1 under 980 nm illumination. The SR-PEC ratiometric strategy showed acceptable stability and reproducibility with a superior anti-interference ability. This approach can provide the guidance for the design of ratiometric, multiplexed, and point-of-care biosensors. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/30543292/Dual_Channel_Photoelectrochemical_Ratiometric_Aptasensor_with_up_Converting_Nanocrystals_Using_Spatial_Resolved_Technique_on_Homemade_3D_Printed_Device_ DB - PRIME DP - Unbound Medicine ER -