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Effects of Toona sinensis Leaf Extract and Its Chemical Constituents on Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats.
Molecules. 2018 Dec 09; 23(12)M

Abstract

Toona sinensis leaf is used as a seasonal vegetable in Korea. A 70% ethanol extract of these leaves exhibited potent xanthine oxidase (XO) inhibition, with a 50% inhibitory concentration (IC50) of 78.4 µM. To investigate the compounds responsible for this effect, bioassay-guided purification led to the isolation of five constituents, identified as quercetin-3-O-rutinoside, quercetin-3-O-β-d-glucopyranoside, 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (compound 3), quercetin-3-O-α-l-rhamnopyranoside, and kaempferol-3-O-α-l-rhamnopyranoside. Compound 3 showed the most potent inhibition of XO, with an IC50 of 2.8 µM. This was similar to that of allopurinol (IC50 = 2.3 µM), which is used clinically to treat hyperuricemia. Kinetic analyses found that compound 3 was a reversible noncompetitive XO inhibitor. In vivo, the T. sinensis leaf extract (300 mg/kg), or compound 3 (40 mg/kg), significantly decreased serum uric acid levels in rats with potassium oxonate-induced hyperuricemia. Furthermore, ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry analysis identified a high level of compound 3 in the leaf extract. These findings suggest that T. sinensis leaves could be developed to produce nutraceutical preparations.

Authors+Show Affiliations

Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Korea. yukhj@kiom.re.kr.Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Korea. rheeys04@kiom.re.kr.Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Chungbuk 28116, Korea. ryuhw@kribb.re.kr.Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, Korea. sksh518@dju.kr.Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Korea. dskim@kiom.re.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30544886

Citation

Yuk, Heung Joo, et al. "Effects of Toona Sinensis Leaf Extract and Its Chemical Constituents On Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats." Molecules (Basel, Switzerland), vol. 23, no. 12, 2018.
Yuk HJ, Lee YS, Ryu HW, et al. Effects of Toona sinensis Leaf Extract and Its Chemical Constituents on Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats. Molecules. 2018;23(12).
Yuk, H. J., Lee, Y. S., Ryu, H. W., Kim, S. H., & Kim, D. S. (2018). Effects of Toona sinensis Leaf Extract and Its Chemical Constituents on Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats. Molecules (Basel, Switzerland), 23(12). https://doi.org/10.3390/molecules23123254
Yuk HJ, et al. Effects of Toona Sinensis Leaf Extract and Its Chemical Constituents On Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats. Molecules. 2018 Dec 9;23(12) PubMed PMID: 30544886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Toona sinensis Leaf Extract and Its Chemical Constituents on Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats. AU - Yuk,Heung Joo, AU - Lee,Young-Sil, AU - Ryu,Hyung Won, AU - Kim,Seung-Hyung, AU - Kim,Dong-Seon, Y1 - 2018/12/09/ PY - 2018/11/15/received PY - 2018/12/06/revised PY - 2018/12/08/accepted PY - 2018/12/15/entrez PY - 2018/12/14/pubmed PY - 2019/3/12/medline KW - Toona sinensis KW - hyperuricemia KW - ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry KW - uric acid KW - xanthine oxidase JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 12 N2 - Toona sinensis leaf is used as a seasonal vegetable in Korea. A 70% ethanol extract of these leaves exhibited potent xanthine oxidase (XO) inhibition, with a 50% inhibitory concentration (IC50) of 78.4 µM. To investigate the compounds responsible for this effect, bioassay-guided purification led to the isolation of five constituents, identified as quercetin-3-O-rutinoside, quercetin-3-O-β-d-glucopyranoside, 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (compound 3), quercetin-3-O-α-l-rhamnopyranoside, and kaempferol-3-O-α-l-rhamnopyranoside. Compound 3 showed the most potent inhibition of XO, with an IC50 of 2.8 µM. This was similar to that of allopurinol (IC50 = 2.3 µM), which is used clinically to treat hyperuricemia. Kinetic analyses found that compound 3 was a reversible noncompetitive XO inhibitor. In vivo, the T. sinensis leaf extract (300 mg/kg), or compound 3 (40 mg/kg), significantly decreased serum uric acid levels in rats with potassium oxonate-induced hyperuricemia. Furthermore, ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry analysis identified a high level of compound 3 in the leaf extract. These findings suggest that T. sinensis leaves could be developed to produce nutraceutical preparations. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30544886/Effects_of_Toona_sinensis_Leaf_Extract_and_Its_Chemical_Constituents_on_Xanthine_Oxidase_Activity_and_Serum_Uric_Acid_Levels_in_Potassium_Oxonate_Induced_Hyperuricemic_Rats_ L2 - http://www.mdpi.com/resolver?pii=molecules23123254 DB - PRIME DP - Unbound Medicine ER -