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Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill.
Int J Mol Sci 2018; 19(12)IJ

Abstract

Hydrogen sulfide (H₂S) has emerged as an important signaling molecule and plays a significant role during different environmental stresses in plants. The present work was carried out to explore the potential role of H₂S in reversal of dehydration stress-inhibited O-acetylserine (thiol) lyase (OAS-TL), l-cysteine desulfhydrase (LCD), and d-cysteine desulfhydrase (DCD) response in arugula (Eruca sativa Mill.) plants. Dehydration-stressed plants exhibited reduced water status and increased levels of hydrogen peroxide (H₂O₂) and superoxide (O₂•-) content that increased membrane permeability and lipid peroxidation, and caused a reduction in chlorophyll content. However, H₂S donor sodium hydrosulfide (NaHS), at the rate of 2 mM, substantially reduced oxidative stress (lower H₂O₂ and O₂•-) by upregulating activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and increasing accumulation of osmolytes viz. proline and glycine betaine (GB). All these, together, resulted in reduced membrane permeability, lipid peroxidation, water loss, and improved hydration level of plants. The beneficial role of H₂S in the tolerance of plants to dehydration stress was traced with H₂S-mediated activation of carbonic anhydrase activity and enzyme involved in the biosynthesis of cysteine (Cys), such as OAS-TL. H₂S-treated plants showed maximum Cys content. The exogenous application of H₂S also induced the activity of LCD and DCD enzymes that assisted the plants to synthesize more H₂S from accumulated Cys. Therefore, an adequate concentration of H₂S was maintained, that improved the efficiency of plants to mitigate dehydration stress-induced alterations. The central role of H₂S in the reversal of dehydration stress-induced damage was evident with the use of the H₂S scavenger, hypotaurine.

Authors+Show Affiliations

Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia. nasirmn4@gmail.com.Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia. falzuaiber@ut.edu.sa.Chair of Climate Change, Environmental Development and Vegetation Cover, Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. aalhuquail@ksu.edu.sa.Chair of Climate Change, Environmental Development and Vegetation Cover, Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. manzerhs@yahoo.co.in.Chair of Climate Change, Environmental Development and Vegetation Cover, Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. hayhassan@ksu.edu.sa.Department of Physics and Chemistry, Faculty of Science, Shaqra Univeristy, Shaqra 15572, Saudi Arabia. malmuwayhi@su.edu.sa.Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. hulhaque@ksu.edu.sa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30544896

Citation

Khan, M Nasir, et al. "Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca Sativa Mill." International Journal of Molecular Sciences, vol. 19, no. 12, 2018.
Khan MN, AlZuaibr FM, Al-Huqail AA, et al. Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill. Int J Mol Sci. 2018;19(12).
Khan, M. N., AlZuaibr, F. M., Al-Huqail, A. A., Siddiqui, M. H., M Ali, H., Al-Muwayhi, M. A., & Al-Haque, H. N. (2018). Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill. International Journal of Molecular Sciences, 19(12), doi:10.3390/ijms19123981.
Khan MN, et al. Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca Sativa Mill. Int J Mol Sci. 2018 Dec 11;19(12) PubMed PMID: 30544896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill. AU - Khan,M Nasir, AU - AlZuaibr,Fahad M, AU - Al-Huqail,Asma A, AU - Siddiqui,Manzer H, AU - M Ali,Hayssam, AU - Al-Muwayhi,Mohammed A, AU - Al-Haque,Hafiz N, Y1 - 2018/12/11/ PY - 2018/11/15/received PY - 2018/12/05/revised PY - 2018/12/05/accepted PY - 2018/12/15/entrez PY - 2018/12/14/pubmed PY - 2019/3/12/medline KW - Eruca sativa KW - antioxidant system KW - dehydration stress KW - hydrogen sulfide KW - osmolytes JF - International journal of molecular sciences JO - Int J Mol Sci VL - 19 IS - 12 N2 - Hydrogen sulfide (H₂S) has emerged as an important signaling molecule and plays a significant role during different environmental stresses in plants. The present work was carried out to explore the potential role of H₂S in reversal of dehydration stress-inhibited O-acetylserine (thiol) lyase (OAS-TL), l-cysteine desulfhydrase (LCD), and d-cysteine desulfhydrase (DCD) response in arugula (Eruca sativa Mill.) plants. Dehydration-stressed plants exhibited reduced water status and increased levels of hydrogen peroxide (H₂O₂) and superoxide (O₂•-) content that increased membrane permeability and lipid peroxidation, and caused a reduction in chlorophyll content. However, H₂S donor sodium hydrosulfide (NaHS), at the rate of 2 mM, substantially reduced oxidative stress (lower H₂O₂ and O₂•-) by upregulating activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and increasing accumulation of osmolytes viz. proline and glycine betaine (GB). All these, together, resulted in reduced membrane permeability, lipid peroxidation, water loss, and improved hydration level of plants. The beneficial role of H₂S in the tolerance of plants to dehydration stress was traced with H₂S-mediated activation of carbonic anhydrase activity and enzyme involved in the biosynthesis of cysteine (Cys), such as OAS-TL. H₂S-treated plants showed maximum Cys content. The exogenous application of H₂S also induced the activity of LCD and DCD enzymes that assisted the plants to synthesize more H₂S from accumulated Cys. Therefore, an adequate concentration of H₂S was maintained, that improved the efficiency of plants to mitigate dehydration stress-induced alterations. The central role of H₂S in the reversal of dehydration stress-induced damage was evident with the use of the H₂S scavenger, hypotaurine. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/30544896/Hydrogen_Sulfide-Mediated_Activation_of_O-Acetylserine_(Thiol)_Lyase_and_l/d-Cysteine_Desulfhydrase_Enhance_Dehydration_Tolerance_in_Eruca_sativa_Mill L2 - http://www.mdpi.com/resolver?pii=ijms19123981 DB - PRIME DP - Unbound Medicine ER -