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Knockdown of lncRNA DLX6-AS1 inhibits cell proliferation, migration and invasion while promotes apoptosis by downregulating PRR11 expression and upregulating miR-144 in non-small cell lung cancer.
Biomed Pharmacother 2019; 109:1851-1859BP

Abstract

BACKGROUND

Long non-coding RNA (lncRNA) distal-less homeobox 6 antisense 1 (DLX6-AS1) was reported to be dysregulated in lung cancer. However, detailed roles of DLX6-AS1 in the pathogenesis of non-small cell lung cancer (NSCLC) were largely unknown.

METHODS

The expression of DLX6-AS1 was measured in NSCLC tissues and cells by quantitative real-time PCR (qRT-PCR). The abundance of proline rich 11 (PRR11) were detected by qRT-PCR and western blot, respectively. The effects of DLX6-AS1 and PRR11 on cell proliferation, migration, invasion and apoptosis were explored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), transwell and flow cytometry analysis, respectively. Luciferase reporter assay, qRT-PCR and western blot were performed to confirm the interaction between miR-144 and DLX6-AS1 or PRR11. Tumor xenograft assay was performed to verify the role of DLX6-AS1 in NSCLC in vivo.

RESULTS

DLX6-AS1 and PRR11 were elevated in NSCLC tissues and cells. DLX6-AS1 was positively correlated with PRR11 mRNA expression in NSCLC tissues. Knockdown of DLX6-AS1 and PRR11 significantly suppressed cell proliferation, migration and invasion and induced apoptosis in NSCLC cells, which was reversed by PRR11 overexpression. In addition, DLX6-AS1 and PRR11 were demonstrated to interact with microRNA-144 (miR-144) and DLX6-AS1 upregulated PRR11 expression by acting as a competing endogenous RNA (ceRNA) of miR-144 in NSCLC cells. Furthermore, DLX6-AS1 knockdown suppressed tumor growth in NSCLC in vivo by upregulating miR-144 and downregulating PRR11.

CONCLUSION

Knockdown of DLX6-AS1 inhibited cell proliferation, migration, invasion and promoted apoptosis by downregulating PRR11 expression and upregulating miR-144 in NSCLC.

Authors+Show Affiliations

Department of Senile Respiratory and Sleep, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.Department Two of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.Department Two of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. Electronic address: wjgoodok5252@sohu.com.Department Five of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30551440

Citation

Huang, Yongjie, et al. "Knockdown of lncRNA DLX6-AS1 Inhibits Cell Proliferation, Migration and Invasion While Promotes Apoptosis By Downregulating PRR11 Expression and Upregulating miR-144 in Non-small Cell Lung Cancer." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 109, 2019, pp. 1851-1859.
Huang Y, Ni R, Wang J, et al. Knockdown of lncRNA DLX6-AS1 inhibits cell proliferation, migration and invasion while promotes apoptosis by downregulating PRR11 expression and upregulating miR-144 in non-small cell lung cancer. Biomed Pharmacother. 2019;109:1851-1859.
Huang, Y., Ni, R., Wang, J., & Liu, Y. (2019). Knockdown of lncRNA DLX6-AS1 inhibits cell proliferation, migration and invasion while promotes apoptosis by downregulating PRR11 expression and upregulating miR-144 in non-small cell lung cancer. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 109, pp. 1851-1859. doi:10.1016/j.biopha.2018.09.151.
Huang Y, et al. Knockdown of lncRNA DLX6-AS1 Inhibits Cell Proliferation, Migration and Invasion While Promotes Apoptosis By Downregulating PRR11 Expression and Upregulating miR-144 in Non-small Cell Lung Cancer. Biomed Pharmacother. 2019;109:1851-1859. PubMed PMID: 30551440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockdown of lncRNA DLX6-AS1 inhibits cell proliferation, migration and invasion while promotes apoptosis by downregulating PRR11 expression and upregulating miR-144 in non-small cell lung cancer. AU - Huang,Yongjie, AU - Ni,Ran, AU - Wang,Jing, AU - Liu,Ying, Y1 - 2018/11/26/ PY - 2018/07/06/received PY - 2018/09/17/revised PY - 2018/09/26/accepted PY - 2018/12/16/entrez PY - 2018/12/16/pubmed PY - 2019/3/28/medline KW - DLX6-AS1 KW - NSCLC KW - PRR11 KW - Proliferation KW - miR-144 SP - 1851 EP - 1859 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 109 N2 - BACKGROUND: Long non-coding RNA (lncRNA) distal-less homeobox 6 antisense 1 (DLX6-AS1) was reported to be dysregulated in lung cancer. However, detailed roles of DLX6-AS1 in the pathogenesis of non-small cell lung cancer (NSCLC) were largely unknown. METHODS: The expression of DLX6-AS1 was measured in NSCLC tissues and cells by quantitative real-time PCR (qRT-PCR). The abundance of proline rich 11 (PRR11) were detected by qRT-PCR and western blot, respectively. The effects of DLX6-AS1 and PRR11 on cell proliferation, migration, invasion and apoptosis were explored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), transwell and flow cytometry analysis, respectively. Luciferase reporter assay, qRT-PCR and western blot were performed to confirm the interaction between miR-144 and DLX6-AS1 or PRR11. Tumor xenograft assay was performed to verify the role of DLX6-AS1 in NSCLC in vivo. RESULTS: DLX6-AS1 and PRR11 were elevated in NSCLC tissues and cells. DLX6-AS1 was positively correlated with PRR11 mRNA expression in NSCLC tissues. Knockdown of DLX6-AS1 and PRR11 significantly suppressed cell proliferation, migration and invasion and induced apoptosis in NSCLC cells, which was reversed by PRR11 overexpression. In addition, DLX6-AS1 and PRR11 were demonstrated to interact with microRNA-144 (miR-144) and DLX6-AS1 upregulated PRR11 expression by acting as a competing endogenous RNA (ceRNA) of miR-144 in NSCLC cells. Furthermore, DLX6-AS1 knockdown suppressed tumor growth in NSCLC in vivo by upregulating miR-144 and downregulating PRR11. CONCLUSION: Knockdown of DLX6-AS1 inhibited cell proliferation, migration, invasion and promoted apoptosis by downregulating PRR11 expression and upregulating miR-144 in NSCLC. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/30551440/Knockdown_of_lncRNA_DLX6_AS1_inhibits_cell_proliferation_migration_and_invasion_while_promotes_apoptosis_by_downregulating_PRR11_expression_and_upregulating_miR_144_in_non_small_cell_lung_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(18)34641-9 DB - PRIME DP - Unbound Medicine ER -