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Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma.
Biomed Pharmacother 2019; 109:798-805BP

Abstract

BACKGROUND

Even though the role of long non-coding RNA H19 (lncRNA-H19) in diverse cancer types has been studied, exact effect of lncRNA-H19 as well as the underlying mechanism in retinoblastoma (RB) is poorly reported. We aimed to explore the possible functions of lncRNA-H19 in human RB Y79 cells.

METHODS

LncRNA-H19 in Y79 cells was silenced, and effects of lncRNA-H19 silence on cell viability, migration and invasion, and apoptosis were analyzed by using trypan blue exclusion, Transwell assay, and flow cytometry assay/Western blot analysis, respectively. Then, miR-143 expression in cells with lncRNA-H19 silence was determined by RT-qPCR, and effects of miR-143 inhibition on lncRNA-H19-suppressing cells were assessed. Whether RUNX2 was a target of miR-143 and the involved signaling pathways in the modulation of miR-143 were also studied.

RESULTS

LncRNA-H19 knockdown repressed cell viability, migration and invasion while promoted apoptosis in Y79 cells. miR-143 was a downstream factor of lncRNA-H19, and its inhibition reversed the effects of lncRNA-H19 silence on Y79 cells. RUNX2 was a target gene of miR-143, and miR-143 was found to affect Y79 cells via down-regulation of RUNX2. Phosphorylation of key kinases related in the PI3K/AKT/mTOR pathways was reduced by miR-143 via regulation of RUNX2.

CONCLUSION

Knockdown of lncRNA-H19 acted a tumor suppressive role in Y79 cells through up-regulating miR-143. Moreover, miR-143 exerted tumor suppressive effects on Y79 cells by targeting RUNX2, along with inhibition of the PI3K/AKT/mTOR pathways.

Authors+Show Affiliations

Department of Ophthalmology, Linyi Central Hospital, Linyi 276400, Shandong, China.Department of Ophthalmology, Qingdao Chengyang People's Hospital, Qingdao, 266109, Shandong, China.Department of Ophthalmology, Linyi Central Hospital, Linyi 276400, Shandong, China. Electronic address: fenghuayu99@sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30551533

Citation

Qi, Defeng, et al. "Knockdown of lncRNA-H19 Inhibits Cell Viability, Migration and Invasion While Promotes Apoptosis Via microRNA-143/RUNX2 Axis in Retinoblastoma." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 109, 2019, pp. 798-805.
Qi D, Wang M, Yu F. Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma. Biomed Pharmacother. 2019;109:798-805.
Qi, D., Wang, M., & Yu, F. (2019). Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 109, pp. 798-805. doi:10.1016/j.biopha.2018.10.096.
Qi D, Wang M, Yu F. Knockdown of lncRNA-H19 Inhibits Cell Viability, Migration and Invasion While Promotes Apoptosis Via microRNA-143/RUNX2 Axis in Retinoblastoma. Biomed Pharmacother. 2019;109:798-805. PubMed PMID: 30551533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma. AU - Qi,Defeng, AU - Wang,Mingming, AU - Yu,Fenghua, Y1 - 2018/11/05/ PY - 2018/06/13/received PY - 2018/10/16/revised PY - 2018/10/18/accepted PY - 2018/12/16/entrez PY - 2018/12/16/pubmed PY - 2019/4/2/medline KW - PI3K/AKT/mTOR KW - RUNX2 KW - Retinoblastoma KW - lncRNA-H19 KW - microRNA-143 SP - 798 EP - 805 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 109 N2 - BACKGROUND: Even though the role of long non-coding RNA H19 (lncRNA-H19) in diverse cancer types has been studied, exact effect of lncRNA-H19 as well as the underlying mechanism in retinoblastoma (RB) is poorly reported. We aimed to explore the possible functions of lncRNA-H19 in human RB Y79 cells. METHODS: LncRNA-H19 in Y79 cells was silenced, and effects of lncRNA-H19 silence on cell viability, migration and invasion, and apoptosis were analyzed by using trypan blue exclusion, Transwell assay, and flow cytometry assay/Western blot analysis, respectively. Then, miR-143 expression in cells with lncRNA-H19 silence was determined by RT-qPCR, and effects of miR-143 inhibition on lncRNA-H19-suppressing cells were assessed. Whether RUNX2 was a target of miR-143 and the involved signaling pathways in the modulation of miR-143 were also studied. RESULTS: LncRNA-H19 knockdown repressed cell viability, migration and invasion while promoted apoptosis in Y79 cells. miR-143 was a downstream factor of lncRNA-H19, and its inhibition reversed the effects of lncRNA-H19 silence on Y79 cells. RUNX2 was a target gene of miR-143, and miR-143 was found to affect Y79 cells via down-regulation of RUNX2. Phosphorylation of key kinases related in the PI3K/AKT/mTOR pathways was reduced by miR-143 via regulation of RUNX2. CONCLUSION: Knockdown of lncRNA-H19 acted a tumor suppressive role in Y79 cells through up-regulating miR-143. Moreover, miR-143 exerted tumor suppressive effects on Y79 cells by targeting RUNX2, along with inhibition of the PI3K/AKT/mTOR pathways. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/30551533/Knockdown_of_lncRNA_H19_inhibits_cell_viability_migration_and_invasion_while_promotes_apoptosis_via_microRNA_143/RUNX2_axis_in_retinoblastoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(18)34025-3 DB - PRIME DP - Unbound Medicine ER -