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The group I GH3 family genes encoding JA-Ile synthetase act as positive regulator in the resistance of rice to Xanthomonas oryzae pv. oryzae.
Biochem Biophys Res Commun 2019; 508(4):1062-1066BB

Abstract

Plant GH3 genes are key components of the hormonal mechanism regulating growth and development, responding to biotic and abiotic stress. GH3 proteins are involved in hormonal homeostasis through conjugation to amino acids of the free form of salicylic acid, jasmonic acid (JA) or indole-3-acetic acid (IAA). Our previous work has uncovered that two GH3 genes encoding IAA-amido synthetase play important roles in the resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) in rice, however, whether other rice GH3 genes play roles in resistance to Xoo is unclear. Here, we validated that GH3.3, GH3.5, GH3.6 and GH3.12, four members of group I GH3 family, are the functional JA-Ile synthetases by catalyzing the conversion of free JA into active form of JA-Ile in vitro and in vivo. The overexpressing plants of four genes individually accumulated less JA but more JA-Ile than the wild type plants. Conversely, the corresponding suppressing plants of four genes contained comparable JA and JA-Ile concentrations, but the triple and quadruple suppressing plants had lower level of JA-Ile compared with wild type plants, suggesting functional redundancy of the same clade of GH3 family. Furthermore, the group I GH3 family genes positively mediated rice resistance to bacterial pathogen Xoo through modulating JA homeostasis and regulating transcription pattern of JA-responsive genes.

Authors+Show Affiliations

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China. Electronic address: myuan@mail.hzau.edu.cn.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China. Electronic address: swang@mail.hzau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30553449

Citation

Hui, Shugang, et al. "The Group I GH3 Family Genes Encoding JA-Ile Synthetase Act as Positive Regulator in the Resistance of Rice to Xanthomonas Oryzae Pv. Oryzae." Biochemical and Biophysical Research Communications, vol. 508, no. 4, 2019, pp. 1062-1066.
Hui S, Hao M, Liu H, et al. The group I GH3 family genes encoding JA-Ile synthetase act as positive regulator in the resistance of rice to Xanthomonas oryzae pv. oryzae. Biochem Biophys Res Commun. 2019;508(4):1062-1066.
Hui, S., Hao, M., Liu, H., Xiao, J., Li, X., Yuan, M., & Wang, S. (2019). The group I GH3 family genes encoding JA-Ile synthetase act as positive regulator in the resistance of rice to Xanthomonas oryzae pv. oryzae. Biochemical and Biophysical Research Communications, 508(4), pp. 1062-1066. doi:10.1016/j.bbrc.2018.12.057.
Hui S, et al. The Group I GH3 Family Genes Encoding JA-Ile Synthetase Act as Positive Regulator in the Resistance of Rice to Xanthomonas Oryzae Pv. Oryzae. Biochem Biophys Res Commun. 2019 01 22;508(4):1062-1066. PubMed PMID: 30553449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The group I GH3 family genes encoding JA-Ile synthetase act as positive regulator in the resistance of rice to Xanthomonas oryzae pv. oryzae. AU - Hui,Shugang, AU - Hao,Mengyu, AU - Liu,Hongbo, AU - Xiao,Jinghua, AU - Li,Xianghua, AU - Yuan,Meng, AU - Wang,Shiping, Y1 - 2018/12/13/ PY - 2018/12/01/received PY - 2018/12/07/accepted PY - 2018/12/17/pubmed PY - 2019/9/12/medline PY - 2018/12/17/entrez KW - GH3 KW - JA KW - JA-Ile KW - Rice KW - Xanthomonas oryzae pv. oryzae SP - 1062 EP - 1066 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 508 IS - 4 N2 - Plant GH3 genes are key components of the hormonal mechanism regulating growth and development, responding to biotic and abiotic stress. GH3 proteins are involved in hormonal homeostasis through conjugation to amino acids of the free form of salicylic acid, jasmonic acid (JA) or indole-3-acetic acid (IAA). Our previous work has uncovered that two GH3 genes encoding IAA-amido synthetase play important roles in the resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) in rice, however, whether other rice GH3 genes play roles in resistance to Xoo is unclear. Here, we validated that GH3.3, GH3.5, GH3.6 and GH3.12, four members of group I GH3 family, are the functional JA-Ile synthetases by catalyzing the conversion of free JA into active form of JA-Ile in vitro and in vivo. The overexpressing plants of four genes individually accumulated less JA but more JA-Ile than the wild type plants. Conversely, the corresponding suppressing plants of four genes contained comparable JA and JA-Ile concentrations, but the triple and quadruple suppressing plants had lower level of JA-Ile compared with wild type plants, suggesting functional redundancy of the same clade of GH3 family. Furthermore, the group I GH3 family genes positively mediated rice resistance to bacterial pathogen Xoo through modulating JA homeostasis and regulating transcription pattern of JA-responsive genes. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/30553449/The_group_I_GH3_family_genes_encoding_JA_Ile_synthetase_act_as_positive_regulator_in_the_resistance_of_rice_to_Xanthomonas_oryzae_pv__oryzae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(18)32700-1 DB - PRIME DP - Unbound Medicine ER -