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Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure of Aegilops uniaristata.
J Genet. 2018 Dec; 97(5):1463-1468.JG

Abstract

Low-molecular-weight glutenin subunits (LMW-GSs) are one of the important factors for the dough processing quality. In this study, a novel LMW-GS, designated LMW-N13, from the wheat relative species Aegilops uniaristata PI 554421 was cloned and characterized. Unlike previously published LMW-GSs, LMW-N13 has a large molecular weight and is the largest LMW-GS published thus far. Sequence alignments demonstrated that LMW-N13 is a LMW-i-type subunit but contains nine cysteine residues which is one more than typical LMW-i-type subunits. In addition, four insertions are present in the repetitive domain that resulted in the large molecular weight. In vitro analysis showed that LMW-N13 could improve the dough quality of different base flours.

Authors+Show Affiliations

Tang H
State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, No. 61. Daizong Street, Taian 271018, Shandong Province, People's Republic of China. lianfei@sdau.edu.cn, lkong@sdau.edu.cn.
Du X
No affiliation info available
Wang H
No affiliation info available
Ma X
No affiliation info available
Bo C
No affiliation info available
Li A
No affiliation info available
Kong L
No affiliation info available

MeSH

AegilopsAmino Acid SequenceBase SequenceCloning, MolecularFlourGenes, PlantGlutensINDEL MutationMolecular WeightPhylogenyPolymorphism, Single NucleotideProtein SubunitsSequence AlignmentSequence Homology, Amino AcidSequence Homology, Nucleic Acid

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30555095

Citation

Tang, Heng, et al. "Cloning and Characterization of a Novel Low-molecular-weight Glutenin Subunit Gene With an Unusual Molecular Structure of Aegilops Uniaristata." Journal of Genetics, vol. 97, no. 5, 2018, pp. 1463-1468.
Tang H, Du X, Wang H, et al. Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure of Aegilops uniaristata. J Genet. 2018;97(5):1463-1468.
Tang, H., Du, X., Wang, H., Ma, X., Bo, C., Li, A., & Kong, L. (2018). Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure of Aegilops uniaristata. Journal of Genetics, 97(5), 1463-1468.
Tang H, et al. Cloning and Characterization of a Novel Low-molecular-weight Glutenin Subunit Gene With an Unusual Molecular Structure of Aegilops Uniaristata. J Genet. 2018;97(5):1463-1468. PubMed PMID: 30555095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure of Aegilops uniaristata. AU - Tang,Heng, AU - Du,Xuye, AU - Wang,Hongwei, AU - Ma,Xin, AU - Bo,Cunyao, AU - Li,Anfei, AU - Kong,Lingrang, PY - 2018/12/18/entrez PY - 2018/12/18/pubmed PY - 2019/2/8/medline SP - 1463 EP - 1468 JF - Journal of genetics JO - J Genet VL - 97 IS - 5 N2 - Low-molecular-weight glutenin subunits (LMW-GSs) are one of the important factors for the dough processing quality. In this study, a novel LMW-GS, designated LMW-N13, from the wheat relative species Aegilops uniaristata PI 554421 was cloned and characterized. Unlike previously published LMW-GSs, LMW-N13 has a large molecular weight and is the largest LMW-GS published thus far. Sequence alignments demonstrated that LMW-N13 is a LMW-i-type subunit but contains nine cysteine residues which is one more than typical LMW-i-type subunits. In addition, four insertions are present in the repetitive domain that resulted in the large molecular weight. In vitro analysis showed that LMW-N13 could improve the dough quality of different base flours. SN - 0973-7731 UR - https://www.unboundmedicine.com/medline/citation/30555095/Cloning_and_characterization_of_a_novel_low_molecular_weight_glutenin_subunit_gene_with_an_unusual_molecular_structure_of_Aegilops_uniaristata_ DB - PRIME DP - Unbound Medicine ER -