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Low expression of microRNA-1266 promotes colorectal cancer progression via targeting FTO.

Abstract

OBJECTIVE

To explore the role of microRNA-1266 in colorectal cancer (CRC) and its underlying mechanism.

PATIENTS AND METHODS

The expression level of microRNA-1266 in 48 CRC tissues and paracancerous tissues was detected by quantitative Real-time-polymerase chain reaction (qRT-PCR). The relationship between microRNA-1266 expression and basic characteristics of CRC patients was analyzed. The effect of microRNA-1266 on the viability of CRC cells was detected by cell counting kit-8 (CCK-8) assay. Subsequently, a potential target gene for microRNA-1266 was predicted through bioinformatics. Finally, the binding condition between microRNA-1266 and the target gene was verified by RNA binding protein immunoprecipitation (RIP) and luciferase reporter gene assay, respectively.

RESULTS

MicroRNA-1266 was lowly expressed in 48 cases of CRC tissues than that of paracancerous tissues. Clinical data demonstrated that microRNA-1266 expression was correlated to tumor size and TNM of CRC patients. Knockdown of microRNA-1266 promoted proliferation of CRC cells. FTO was predicted to be the target gene for microRNA-1266, which was negatively regulated by microRNA-1266.

CONCLUSIONS

MicroRNA-1266 is lowly expressed in CRC tissues than that of paracancerous tissues. Lowly expressed microRNA-1266 promotes the occurrence and progression of CRC by directly targeting FTO.

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  • Authors+Show Affiliations

    ,

    Department of Anorectal Surgery, The First People's Hospital of Wujiang District, Suzhou, Jiangsu, China. 24500265@qq.com.

    , , , ,

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30556861

    Citation

    Shen, X-P, et al. "Low Expression of microRNA-1266 Promotes Colorectal Cancer Progression Via Targeting FTO." European Review for Medical and Pharmacological Sciences, vol. 22, no. 23, 2018, pp. 8220-8226.
    Shen XP, Ling X, Lu H, et al. Low expression of microRNA-1266 promotes colorectal cancer progression via targeting FTO. Eur Rev Med Pharmacol Sci. 2018;22(23):8220-8226.
    Shen, X. P., Ling, X., Lu, H., Zhou, C. X., Zhang, J. K., & Yu, Q. (2018). Low expression of microRNA-1266 promotes colorectal cancer progression via targeting FTO. European Review for Medical and Pharmacological Sciences, 22(23), pp. 8220-8226. doi:10.26355/eurrev_201812_16516.
    Shen XP, et al. Low Expression of microRNA-1266 Promotes Colorectal Cancer Progression Via Targeting FTO. Eur Rev Med Pharmacol Sci. 2018;22(23):8220-8226. PubMed PMID: 30556861.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Low expression of microRNA-1266 promotes colorectal cancer progression via targeting FTO. AU - Shen,X-P, AU - Ling,X, AU - Lu,H, AU - Zhou,C-X, AU - Zhang,J-K, AU - Yu,Q, PY - 2018/12/18/entrez PY - 2018/12/18/pubmed PY - 2018/12/18/medline SP - 8220 EP - 8226 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 23 N2 - OBJECTIVE: To explore the role of microRNA-1266 in colorectal cancer (CRC) and its underlying mechanism. PATIENTS AND METHODS: The expression level of microRNA-1266 in 48 CRC tissues and paracancerous tissues was detected by quantitative Real-time-polymerase chain reaction (qRT-PCR). The relationship between microRNA-1266 expression and basic characteristics of CRC patients was analyzed. The effect of microRNA-1266 on the viability of CRC cells was detected by cell counting kit-8 (CCK-8) assay. Subsequently, a potential target gene for microRNA-1266 was predicted through bioinformatics. Finally, the binding condition between microRNA-1266 and the target gene was verified by RNA binding protein immunoprecipitation (RIP) and luciferase reporter gene assay, respectively. RESULTS: MicroRNA-1266 was lowly expressed in 48 cases of CRC tissues than that of paracancerous tissues. Clinical data demonstrated that microRNA-1266 expression was correlated to tumor size and TNM of CRC patients. Knockdown of microRNA-1266 promoted proliferation of CRC cells. FTO was predicted to be the target gene for microRNA-1266, which was negatively regulated by microRNA-1266. CONCLUSIONS: MicroRNA-1266 is lowly expressed in CRC tissues than that of paracancerous tissues. Lowly expressed microRNA-1266 promotes the occurrence and progression of CRC by directly targeting FTO. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30556861/Low_expression_of_microRNA_1266_promotes_colorectal_cancer_progression_via_targeting_FTO_ L2 - https://www.europeanreview.org/article/16516 DB - PRIME DP - Unbound Medicine ER -