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Development and evaluation of Chrysin-Phospholipid complex loaded solid lipid nanoparticles - storage stability and in vitro anti-cancer activity.
J Microencapsul. 2018 Sep; 35(6):600-617.JM

Abstract

CONTEXT

Flavonoids show promising anticancer potential but it is limited due to poor solubility.

OBJECTIVE

The present investigation was to prepare Chrysin-Phospholipid complex loaded solid lipid nanoparticles (Ch-PC-SLNs) for improving its encapsulation as compared to that of Chrysin loaded SLNs (Ch-SLNs) and evaluated for potential increase in the anti-cancer activity against MCF-7 cell line.

METHODS

The physiochemical characteristics and release kinetics for Ch-SLNs and Ch-PC-SLNs were evaluated and compared. Storage stability of Ch-PC-SLNs was evaluated up to 3 months. Solid state properties (DSC, XRD) and morphology (AFM) of Ch-PC-SLNs were also studied. In-vitro anticancer activity was investigated by using MTT assay.

RESULTS AND DISCUSSION

Ch-PC-SLNs exhibited higher encapsulation efficiency than Ch-SLNs and zero order release kinetics. Ch-PC-SLNs were found to be stable upto 3 months upon lyophilisation with mannitol as cryoprotectant. DSC and XRD study revealed the loss of highly crystalline nature of Chrysin in Ch-PC-SLNs. The Ch-PC-SLNs lead to significantly higher in-vitro anticancer activity than that of bulk Chrysin.

CONCLUSION

The study concludes that phospholipids complex with Chrysin lead to improve encapsulation, storage stability of SLNs and in vitro anticancer activity.

Authors+Show Affiliations

a Department of Pharmaceutics , National Institute of Pharmaceutical Education and Research (NIPER) , Raebareli , Uttar Pradesh , India.a Department of Pharmaceutics , National Institute of Pharmaceutical Education and Research (NIPER) , Raebareli , Uttar Pradesh , India. b Department of Pharmaceutics, Institute of Pharmaceutical Research , GLA University , Mathura , Uttar Pradesh , India.c Divisions of Metabolism and Pharmacokinetics , CSIR-Central Drug Research Institute , Lucknow , Uttar Pradesh , India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30557060

Citation

Komath, Shahadali, et al. "Development and Evaluation of Chrysin-Phospholipid Complex Loaded Solid Lipid Nanoparticles - Storage Stability and in Vitro Anti-cancer Activity." Journal of Microencapsulation, vol. 35, no. 6, 2018, pp. 600-617.
Komath S, Garg A, Wahajuddin M. Development and evaluation of Chrysin-Phospholipid complex loaded solid lipid nanoparticles - storage stability and in vitro anti-cancer activity. J Microencapsul. 2018;35(6):600-617.
Komath, S., Garg, A., & Wahajuddin, M. (2018). Development and evaluation of Chrysin-Phospholipid complex loaded solid lipid nanoparticles - storage stability and in vitro anti-cancer activity. Journal of Microencapsulation, 35(6), 600-617. https://doi.org/10.1080/02652048.2018.1559369
Komath S, Garg A, Wahajuddin M. Development and Evaluation of Chrysin-Phospholipid Complex Loaded Solid Lipid Nanoparticles - Storage Stability and in Vitro Anti-cancer Activity. J Microencapsul. 2018;35(6):600-617. PubMed PMID: 30557060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and evaluation of Chrysin-Phospholipid complex loaded solid lipid nanoparticles - storage stability and in vitro anti-cancer activity. AU - Komath,Shahadali, AU - Garg,Anuj, AU - Wahajuddin,Muhammad, PY - 2018/12/18/pubmed PY - 2019/6/14/medline PY - 2018/12/18/entrez KW - Breast cancer (MCF-7) cell line KW - Chrysin KW - Chrysin-Phospholipid complex loaded SLNs KW - freeze drying KW - intracellular reactive oxygen species (ROS) KW - solid lipid nanoparticles SP - 600 EP - 617 JF - Journal of microencapsulation JO - J Microencapsul VL - 35 IS - 6 N2 - CONTEXT: Flavonoids show promising anticancer potential but it is limited due to poor solubility. OBJECTIVE: The present investigation was to prepare Chrysin-Phospholipid complex loaded solid lipid nanoparticles (Ch-PC-SLNs) for improving its encapsulation as compared to that of Chrysin loaded SLNs (Ch-SLNs) and evaluated for potential increase in the anti-cancer activity against MCF-7 cell line. METHODS: The physiochemical characteristics and release kinetics for Ch-SLNs and Ch-PC-SLNs were evaluated and compared. Storage stability of Ch-PC-SLNs was evaluated up to 3 months. Solid state properties (DSC, XRD) and morphology (AFM) of Ch-PC-SLNs were also studied. In-vitro anticancer activity was investigated by using MTT assay. RESULTS AND DISCUSSION: Ch-PC-SLNs exhibited higher encapsulation efficiency than Ch-SLNs and zero order release kinetics. Ch-PC-SLNs were found to be stable upto 3 months upon lyophilisation with mannitol as cryoprotectant. DSC and XRD study revealed the loss of highly crystalline nature of Chrysin in Ch-PC-SLNs. The Ch-PC-SLNs lead to significantly higher in-vitro anticancer activity than that of bulk Chrysin. CONCLUSION: The study concludes that phospholipids complex with Chrysin lead to improve encapsulation, storage stability of SLNs and in vitro anticancer activity. SN - 1464-5246 UR - https://www.unboundmedicine.com/medline/citation/30557060/Development_and_evaluation_of_Chrysin_Phospholipid_complex_loaded_solid_lipid_nanoparticles___storage_stability_and_in_vitro_anti_cancer_activity_ L2 - http://www.tandfonline.com/doi/full/10.1080/02652048.2018.1559369 DB - PRIME DP - Unbound Medicine ER -