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Genome-wide association mapping of spot blotch resistance in wheat association mapping initiative (WAMI) panel of spring wheat (Triticum aestivum L.).
PLoS One. 2018; 13(12):e0208196.Plos

Abstract

Spot blotch (SB) caused by Bipolaris sorokiniana, is one of the most important diseases of wheat in the eastern part of south Asia causing considerable yield loss to the wheat crop. There is an urgent need to identify genetic loci closely associated with resistance to this pathogen for developing resistant cultivars. Hence, genomic regions responsible for SB resistance were searched using a wheat association mapping initiative (WAMI) panel involving 287 spring wheat genotypes of different origin. Genome-wide association mapping (GWAM) was performed using single nucleotide polymorphism (SNP) markers from a custom 90 K wheat SNP array. A mixed linear model (MLM) was used for assessing the association of SNP markers with spot blotch resistance in three consecutive years. Three traits were measured: incubation period, lesion number and area under the disease progress curve (AUDPC). Significant SNP markers were found linked to five, six and four quantitative trait loci (QTLs) for incubation period, lesion number and AUDPC respectively. They were detected on 11 different chromosomes: 1A, 1B, 1D, 4A, 5A, 5B, 6A, 6B, 6D, 7A, 7B with marker R2 range of 0.083 to 0.11. The greatest number of significant SNP-markers was found for lesion number and AUDPC on chromosome 6B and 5B, respectively, representing a better coverage of B-genome by SNPs. On the other hand, the most significant and largest SNP markers for incubation period were detected on 6A and 4A chromosomes indicating that this trait is associated with the A-genome of wheat. Although, QTLs for spot blotch resistance have been reported in wheat on these same chromosomes, the association of incubation period and lesion number with SB resistance has not been reported in previous studies. The panel exhibits considerable variation for SB resistance and also provides a good scope of marker-assisted selection using the identified SNP markers linked to resistant QTLs.

Authors+Show Affiliations

Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.ICAR- Indian Agricultural Statistics Research Institute, New Delhi, India.ICAR- Indian Agricultural Statistics Research Institute, New Delhi, India.ICAR- National Bureau of Plant Genetic Resources, New Delhi, India.Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.International Maize and Wheat Improvement Center (CIMMYT), DPS Marg, New Delhi, India. Borlaug Institute for South Asia (BISA), DPS Marg, New Delhi, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30557301

Citation

Ahirwar, Ram Narayan, et al. "Genome-wide Association Mapping of Spot Blotch Resistance in Wheat Association Mapping Initiative (WAMI) Panel of Spring Wheat (Triticum Aestivum L.)." PloS One, vol. 13, no. 12, 2018, pp. e0208196.
Ahirwar RN, Mishra VK, Chand R, et al. Genome-wide association mapping of spot blotch resistance in wheat association mapping initiative (WAMI) panel of spring wheat (Triticum aestivum L.). PLoS One. 2018;13(12):e0208196.
Ahirwar, R. N., Mishra, V. K., Chand, R., Budhlakoti, N., Mishra, D. C., Kumar, S., Singh, S., & Joshi, A. K. (2018). Genome-wide association mapping of spot blotch resistance in wheat association mapping initiative (WAMI) panel of spring wheat (Triticum aestivum L.). PloS One, 13(12), e0208196. https://doi.org/10.1371/journal.pone.0208196
Ahirwar RN, et al. Genome-wide Association Mapping of Spot Blotch Resistance in Wheat Association Mapping Initiative (WAMI) Panel of Spring Wheat (Triticum Aestivum L.). PLoS One. 2018;13(12):e0208196. PubMed PMID: 30557301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide association mapping of spot blotch resistance in wheat association mapping initiative (WAMI) panel of spring wheat (Triticum aestivum L.). AU - Ahirwar,Ram Narayan, AU - Mishra,Vinod Kumar, AU - Chand,Ramesh, AU - Budhlakoti,Neeraj, AU - Mishra,Dwijesh Chandra, AU - Kumar,Sundeep, AU - Singh,Shweta, AU - Joshi,Arun Kumar, Y1 - 2018/12/17/ PY - 2018/07/20/received PY - 2018/11/13/accepted PY - 2018/12/18/entrez PY - 2018/12/18/pubmed PY - 2019/5/23/medline SP - e0208196 EP - e0208196 JF - PloS one JO - PLoS One VL - 13 IS - 12 N2 - Spot blotch (SB) caused by Bipolaris sorokiniana, is one of the most important diseases of wheat in the eastern part of south Asia causing considerable yield loss to the wheat crop. There is an urgent need to identify genetic loci closely associated with resistance to this pathogen for developing resistant cultivars. Hence, genomic regions responsible for SB resistance were searched using a wheat association mapping initiative (WAMI) panel involving 287 spring wheat genotypes of different origin. Genome-wide association mapping (GWAM) was performed using single nucleotide polymorphism (SNP) markers from a custom 90 K wheat SNP array. A mixed linear model (MLM) was used for assessing the association of SNP markers with spot blotch resistance in three consecutive years. Three traits were measured: incubation period, lesion number and area under the disease progress curve (AUDPC). Significant SNP markers were found linked to five, six and four quantitative trait loci (QTLs) for incubation period, lesion number and AUDPC respectively. They were detected on 11 different chromosomes: 1A, 1B, 1D, 4A, 5A, 5B, 6A, 6B, 6D, 7A, 7B with marker R2 range of 0.083 to 0.11. The greatest number of significant SNP-markers was found for lesion number and AUDPC on chromosome 6B and 5B, respectively, representing a better coverage of B-genome by SNPs. On the other hand, the most significant and largest SNP markers for incubation period were detected on 6A and 4A chromosomes indicating that this trait is associated with the A-genome of wheat. Although, QTLs for spot blotch resistance have been reported in wheat on these same chromosomes, the association of incubation period and lesion number with SB resistance has not been reported in previous studies. The panel exhibits considerable variation for SB resistance and also provides a good scope of marker-assisted selection using the identified SNP markers linked to resistant QTLs. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30557301/Genome_wide_association_mapping_of_spot_blotch_resistance_in_wheat_association_mapping_initiative__WAMI__panel_of_spring_wheat__Triticum_aestivum_L___ L2 - https://dx.plos.org/10.1371/journal.pone.0208196 DB - PRIME DP - Unbound Medicine ER -