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Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source.
Molecules. 2018 Dec 14; 23(12)M

Abstract

Dendropanax morbifera H. Levis a medicinal plant native to South Korea, East Asia, and South America. Among some 75 species, one species grows in Korea. In previous studies, D. morbifera extracts with anti-oxidant, anti-inflammatory, anti-complementary and anti-cancer activities were reported. The present study aims to investigate optimization of extraction and evaluation of anti-hyperuricemic effects of D. morbifera leaf and the phytochemicals contained therein. Ethanol and hexane extract were found to display the best xanthine oxidase inhibition among six types of solvent and water extract. The antioxidant effect of the ethanol extract was superior to that of the hexane extract. The DPPH radical scavenging effect of the ethanol and hexane extracts were 81.52 ± 1.57% and 2.69 ± 0.16. The reducing power of the ethanol and hexane extracts were 9.71 ± 0.15 and 0.89 ± 0.01 mg/g equivalent of gallic acid. Total phenols of the ethanol and hexane extracts were 6.53 ± 0.16 and 0.63 ± 0.001 mg/g equivalent of gallic acid. In addition, we compared the two marker compounds from D. morbifera, chlorogenic acid and rutin, which were determined in the ethanol extract at 0.80 ± 0.03% and 0.52 ± 0.01%, respectively. We found that the ethanol extracts showed better xanthine oxidase inhibition than hexane extracts. Especially, ethanol extracts showed higher antioxidant activity than hexane extracts. Based on these results, we selected the ethanol extract as an effective xanthine oxidase inhibitor and confirmed whether ethanol extracts showed xanthine oxidase inhibition in animal experiments. The in vivo mouse study demonstrated that ethanol extract of D. morbifera leaf at the dose of 300 mg/kg could inhibit blood/hepatic xanthine oxidase activity and this result shows that the xanthine oxidase inhibitory activity in vitro is reproduced in vivo. The present study showed that ethanol extract was optimal xanthine oxidase inhibitor which can be applied to prevent diseases related to hyperuricemia.

Authors+Show Affiliations

Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, Korea. sscho@mokpo.ac.kr.Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, Korea. tmdgml7898@naver.com.Jeonnam Institute of Natural Resources Research, Jangheung-gun, Jeonnam 57922, Korea. blockstar@hanmail.net.Department of Parmaceutical Engineering, Dongshin University, Naju, Jeonnam 58245, Korea. parkkm@dsu.ac.kr.Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, Korea. s1004jh@gmail.com.Department of Nursing, Dongshin University, Naju, Jeonnam 58245, Korea. dhj1221@hanmail.net.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30558104

Citation

Cho, Seung-Sik, et al. "Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax Morbifera H. Lev as an Anti-Hyperuricemic Source." Molecules (Basel, Switzerland), vol. 23, no. 12, 2018.
Cho SS, Song SH, Choi CY, et al. Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source. Molecules. 2018;23(12).
Cho, S. S., Song, S. H., Choi, C. Y., Park, K. M., Shim, J. H., & Park, D. H. (2018). Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source. Molecules (Basel, Switzerland), 23(12). https://doi.org/10.3390/molecules23123313
Cho SS, et al. Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax Morbifera H. Lev as an Anti-Hyperuricemic Source. Molecules. 2018 Dec 14;23(12) PubMed PMID: 30558104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source. AU - Cho,Seung-Sik, AU - Song,Seung-Hui, AU - Choi,Chul-Yung, AU - Park,Kyung Mok, AU - Shim,Jung-Hyun, AU - Park,Dae-Hun, Y1 - 2018/12/14/ PY - 2018/11/01/received PY - 2018/12/03/revised PY - 2018/12/13/accepted PY - 2018/12/19/entrez PY - 2018/12/19/pubmed PY - 2019/3/21/medline KW - Dendropanax morbifera leaf KW - HPLC KW - hyperuricemia KW - xanthine oxidase JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 12 N2 - Dendropanax morbifera H. Levis a medicinal plant native to South Korea, East Asia, and South America. Among some 75 species, one species grows in Korea. In previous studies, D. morbifera extracts with anti-oxidant, anti-inflammatory, anti-complementary and anti-cancer activities were reported. The present study aims to investigate optimization of extraction and evaluation of anti-hyperuricemic effects of D. morbifera leaf and the phytochemicals contained therein. Ethanol and hexane extract were found to display the best xanthine oxidase inhibition among six types of solvent and water extract. The antioxidant effect of the ethanol extract was superior to that of the hexane extract. The DPPH radical scavenging effect of the ethanol and hexane extracts were 81.52 ± 1.57% and 2.69 ± 0.16. The reducing power of the ethanol and hexane extracts were 9.71 ± 0.15 and 0.89 ± 0.01 mg/g equivalent of gallic acid. Total phenols of the ethanol and hexane extracts were 6.53 ± 0.16 and 0.63 ± 0.001 mg/g equivalent of gallic acid. In addition, we compared the two marker compounds from D. morbifera, chlorogenic acid and rutin, which were determined in the ethanol extract at 0.80 ± 0.03% and 0.52 ± 0.01%, respectively. We found that the ethanol extracts showed better xanthine oxidase inhibition than hexane extracts. Especially, ethanol extracts showed higher antioxidant activity than hexane extracts. Based on these results, we selected the ethanol extract as an effective xanthine oxidase inhibitor and confirmed whether ethanol extracts showed xanthine oxidase inhibition in animal experiments. The in vivo mouse study demonstrated that ethanol extract of D. morbifera leaf at the dose of 300 mg/kg could inhibit blood/hepatic xanthine oxidase activity and this result shows that the xanthine oxidase inhibitory activity in vitro is reproduced in vivo. The present study showed that ethanol extract was optimal xanthine oxidase inhibitor which can be applied to prevent diseases related to hyperuricemia. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30558104/Optimization_of_the_Extraction_Conditions_and_Biological_Evaluation_of_Dendropanax_morbifera_H__Lev_as_an_Anti_Hyperuricemic_Source_ L2 - http://www.mdpi.com/resolver?pii=molecules23123313 DB - PRIME DP - Unbound Medicine ER -