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Role of thymoquinone and ebselen in the prevention of sodium arsenite-induced nephrotoxicity in female rats.
Hum Exp Toxicol. 2019 Apr; 38(4):482-493.HE

Abstract

The aim of this study is to investigate the protective effects of thymoquinone (TQ) and ebselen (Eb) on arsenic (As)-induced renal toxicity in female rats. Sodium arsenite was orally administrated at a dose of 20 mg/kg body weight daily for 28 days, either alone or 1 h before TQ (10 mg/kg) or Eb (5 mg/kg) administration. Renal tissue As concentration and oxidative stress markers, including lipid peroxidation (LPO), nitrite/nitrate, and glutathione (GSH) levels, were determined. In addition to the oxidative stress response, antioxidant enzyme activities including that of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were measured. Exposure to As elicited a significant increase in As concentration and significant modifications to the redox state of the kidney, as was evidenced by a significant elevation in LPO and nitrite/nitrate concentration, with a concomitant reduction in GSH content and antioxidant enzyme activity. The oxidant/antioxidant imbalance observed in As toxicity was associated with a significant elevation in renal tumor necrosis factor α, interleukin 6, B-cell lymphoma 2 (Bcl-2)-associated X protein, and caspase 3 levels, in addition to a significant decrease in Bcl-2 levels. Post-administration of TQ and Eb markedly prevented As-induced oxidative stress, inflammation, apoptosis, and As accumulation in the renal tissue and reduced histological renal damage. These findings demonstrate that TQ, the main bioactive phytochemical constituent of Nigella sativa seed oil, and Eb, an organoselenium compound, could significantly inhibit As-induced oxidative damage, apoptosis, and inflammation, and significantly attenuate the accumulation of As in renal tissues by facilitating As biomethylation and excretion.

Authors+Show Affiliations

1 Department of Human Anatomy, College of Medicine, Taif University, Ta'if, Saudi Arabia.2 Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, Egypt.2 Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, Egypt.3 Department of Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam. 4 Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam.5 Department of Biochemistry and Molecular Biology, Helwan University, Helwan, Egypt.2 Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30558456

Citation

Al-Brakati, A Y., et al. "Role of Thymoquinone and Ebselen in the Prevention of Sodium Arsenite-induced Nephrotoxicity in Female Rats." Human & Experimental Toxicology, vol. 38, no. 4, 2019, pp. 482-493.
Al-Brakati AY, Kassab RB, Lokman MS, et al. Role of thymoquinone and ebselen in the prevention of sodium arsenite-induced nephrotoxicity in female rats. Hum Exp Toxicol. 2019;38(4):482-493.
Al-Brakati, A. Y., Kassab, R. B., Lokman, M. S., Elmahallawy, E. K., Amin, H. K., & Abdel Moneim, A. E. (2019). Role of thymoquinone and ebselen in the prevention of sodium arsenite-induced nephrotoxicity in female rats. Human & Experimental Toxicology, 38(4), 482-493. https://doi.org/10.1177/0960327118818246
Al-Brakati AY, et al. Role of Thymoquinone and Ebselen in the Prevention of Sodium Arsenite-induced Nephrotoxicity in Female Rats. Hum Exp Toxicol. 2019;38(4):482-493. PubMed PMID: 30558456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of thymoquinone and ebselen in the prevention of sodium arsenite-induced nephrotoxicity in female rats. AU - Al-Brakati,A Y, AU - Kassab,R B, AU - Lokman,M S, AU - Elmahallawy,E K, AU - Amin,H K, AU - Abdel Moneim,A E, Y1 - 2018/12/17/ PY - 2018/12/19/pubmed PY - 2019/8/14/medline PY - 2018/12/19/entrez KW - Arsenite KW - ebselen KW - inflammation KW - kidney KW - oxidative stress KW - thymoquinone SP - 482 EP - 493 JF - Human & experimental toxicology JO - Hum Exp Toxicol VL - 38 IS - 4 N2 - The aim of this study is to investigate the protective effects of thymoquinone (TQ) and ebselen (Eb) on arsenic (As)-induced renal toxicity in female rats. Sodium arsenite was orally administrated at a dose of 20 mg/kg body weight daily for 28 days, either alone or 1 h before TQ (10 mg/kg) or Eb (5 mg/kg) administration. Renal tissue As concentration and oxidative stress markers, including lipid peroxidation (LPO), nitrite/nitrate, and glutathione (GSH) levels, were determined. In addition to the oxidative stress response, antioxidant enzyme activities including that of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were measured. Exposure to As elicited a significant increase in As concentration and significant modifications to the redox state of the kidney, as was evidenced by a significant elevation in LPO and nitrite/nitrate concentration, with a concomitant reduction in GSH content and antioxidant enzyme activity. The oxidant/antioxidant imbalance observed in As toxicity was associated with a significant elevation in renal tumor necrosis factor α, interleukin 6, B-cell lymphoma 2 (Bcl-2)-associated X protein, and caspase 3 levels, in addition to a significant decrease in Bcl-2 levels. Post-administration of TQ and Eb markedly prevented As-induced oxidative stress, inflammation, apoptosis, and As accumulation in the renal tissue and reduced histological renal damage. These findings demonstrate that TQ, the main bioactive phytochemical constituent of Nigella sativa seed oil, and Eb, an organoselenium compound, could significantly inhibit As-induced oxidative damage, apoptosis, and inflammation, and significantly attenuate the accumulation of As in renal tissues by facilitating As biomethylation and excretion. SN - 1477-0903 UR - https://www.unboundmedicine.com/medline/citation/30558456/Role_of_thymoquinone_and_ebselen_in_the_prevention_of_sodium_arsenite_induced_nephrotoxicity_in_female_rats_ L2 - https://journals.sagepub.com/doi/10.1177/0960327118818246?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -