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Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil.
Mycoses. 2019 Mar; 62(3):261-267.M

Abstract

BACKGROUND

Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation.

OBJECTIVES

To evaluate the role of WB for antibody detection in the diagnosis of histoplasmosis among HIV/AIDS patients.

PATIENTS AND METHODS

Fifty patients with proven or probable histoplasmosis were included. Clinical, epidemiological and laboratory data were described in the same population after a review of their medical records. WB was performed using deglycosylated histoplasmin.

RESULTS

About 82% of patients were adult males and the mean age was 39.3 years. CD4+ T lymphocyte count less than 150 cells/mm3 was observed in 62% patients. Antibodies against Histoplasma capsulatum M antigen were detected in 62% of patients, and against both M and H antigens in 28% of individuals. Sera from 10% of patients were nonreactive. Histoplasmosis was the first opportunistic infection in 38% of the cases. Disseminated and pulmonary histoplasmosis occurred in 84% and 16% of patients, respectively. The overall mortality was 16%.

CONCLUSION

WB could be useful for the histoplasmosis diagnosis in HIV/AIDS patients because of its easefulness and good sensitivity in a population where antibody production is hampered.

Authors+Show Affiliations

Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.Hospital São José de Doenças Infecciosas, Fortaleza, CE, Brazil.Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

30561870

Citation

Almeida, Marcos Abreu, et al. "Role of Western Blot Assay for the Diagnosis of Histoplasmosis in AIDS Patients From a National Institute of Infectious Diseases in Rio De Janeiro, Brazil." Mycoses, vol. 62, no. 3, 2019, pp. 261-267.
Almeida MA, Damasceno LS, Pizzini CV, et al. Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. Mycoses. 2019;62(3):261-267.
Almeida, M. A., Damasceno, L. S., Pizzini, C. V., Muniz, M. M., Almeida-Paes, R., & Zancopé-Oliveira, R. M. (2019). Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. Mycoses, 62(3), 261-267. https://doi.org/10.1111/myc.12877
Almeida MA, et al. Role of Western Blot Assay for the Diagnosis of Histoplasmosis in AIDS Patients From a National Institute of Infectious Diseases in Rio De Janeiro, Brazil. Mycoses. 2019;62(3):261-267. PubMed PMID: 30561870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. AU - Almeida,Marcos Abreu, AU - Damasceno,Lisandra Serra, AU - Pizzini,Cláudia Vera, AU - Muniz,Mauro de Medeiros, AU - Almeida-Paes,Rodrigo, AU - Zancopé-Oliveira,Rosely Maria, Y1 - 2019/01/22/ PY - 2018/10/26/received PY - 2018/12/07/revised PY - 2018/12/10/accepted PY - 2018/12/19/pubmed PY - 2019/3/19/medline PY - 2018/12/19/entrez KW - HIV/AIDS patients KW - antibody detection KW - histoplasmosis KW - immunodiagnosis KW - western blot SP - 261 EP - 267 JF - Mycoses JO - Mycoses VL - 62 IS - 3 N2 - BACKGROUND: Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation. OBJECTIVES: To evaluate the role of WB for antibody detection in the diagnosis of histoplasmosis among HIV/AIDS patients. PATIENTS AND METHODS: Fifty patients with proven or probable histoplasmosis were included. Clinical, epidemiological and laboratory data were described in the same population after a review of their medical records. WB was performed using deglycosylated histoplasmin. RESULTS: About 82% of patients were adult males and the mean age was 39.3 years. CD4+ T lymphocyte count less than 150 cells/mm3 was observed in 62% patients. Antibodies against Histoplasma capsulatum M antigen were detected in 62% of patients, and against both M and H antigens in 28% of individuals. Sera from 10% of patients were nonreactive. Histoplasmosis was the first opportunistic infection in 38% of the cases. Disseminated and pulmonary histoplasmosis occurred in 84% and 16% of patients, respectively. The overall mortality was 16%. CONCLUSION: WB could be useful for the histoplasmosis diagnosis in HIV/AIDS patients because of its easefulness and good sensitivity in a population where antibody production is hampered. SN - 1439-0507 UR - https://www.unboundmedicine.com/medline/citation/30561870/Role_of_western_blot_assay_for_the_diagnosis_of_histoplasmosis_in_AIDS_patients_from_a_National_Institute_of_Infectious_Diseases_in_Rio_de_Janeiro_Brazil_ L2 - https://doi.org/10.1111/myc.12877 DB - PRIME DP - Unbound Medicine ER -