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Genomic Tandem Quadruplication is Associated with Ketoconazole Resistance in Malassezia pachydermatis.
J Microbiol Biotechnol. 2018 Nov 28; 28(11):1937-1945.JM

Abstract

Malassezia pachydermatis is a commensal yeast found on the skin of dogs. However, M. pachydermatis is also considered an opportunistic pathogen and is associated with various canine skin diseases including otitis externa and atopic dermatitis, which usually require treatment using an azole antifungal drug, such as ketoconazole. In this study, we isolated a ketoconazole-resistant strain of M. pachydermatis, designated "KCTC 27587," from the external ear canal of a dog with otitis externa and analyzed its resistance mechanism. To understand the mechanism underlying ketoconazole resistance of the clinical isolate M. pachydermatis KCTC 27587, the whole genome of the yeast was sequenced using the PacBio platform and was compared with M. pachydermatis type strain CBS 1879. We found that a ~84-kb region in chromosome 4 of M. pachydermatis KCTC 27587 was tandemly quadruplicated. The quadruplicated region contains 52 protein coding genes, including the homologs of ERG4 and ERG11, whose overexpression is known to be associated with azole resistance. Our data suggest that the quadruplication of the ~84-kb region may be the cause of the ketoconazole resistance in M. pachydermatis KCTC 27587.

Authors+Show Affiliations

Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.Korea Polar Research Institute, Incheon 21990, Republic of Korea.Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.Haemaru Small Animal Research Institute, Sungnam 13590, Republic of Korea.Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30562885

Citation

Kim, Minchul, et al. "Genomic Tandem Quadruplication Is Associated With Ketoconazole Resistance in Malassezia Pachydermatis." Journal of Microbiology and Biotechnology, vol. 28, no. 11, 2018, pp. 1937-1945.
Kim M, Cho YJ, Park M, et al. Genomic Tandem Quadruplication is Associated with Ketoconazole Resistance in Malassezia pachydermatis. J Microbiol Biotechnol. 2018;28(11):1937-1945.
Kim, M., Cho, Y. J., Park, M., Choi, Y., Hwang, S. Y., & Jung, W. H. (2018). Genomic Tandem Quadruplication is Associated with Ketoconazole Resistance in Malassezia pachydermatis. Journal of Microbiology and Biotechnology, 28(11), 1937-1945. https://doi.org/10.4014/jmb.1810.10019
Kim M, et al. Genomic Tandem Quadruplication Is Associated With Ketoconazole Resistance in Malassezia Pachydermatis. J Microbiol Biotechnol. 2018 Nov 28;28(11):1937-1945. PubMed PMID: 30562885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic Tandem Quadruplication is Associated with Ketoconazole Resistance in Malassezia pachydermatis. AU - Kim,Minchul, AU - Cho,Yong-Joon, AU - Park,Minji, AU - Choi,Yoojeong, AU - Hwang,Sun Young, AU - Jung,Won Hee, PY - 2018/12/19/entrez PY - 2018/12/20/pubmed PY - 2019/6/15/medline KW - Malassezia pachydermatis KW - antifungal KW - dog KW - genomic quadruplication KW - ketoconazole KW - resistance SP - 1937 EP - 1945 JF - Journal of microbiology and biotechnology JO - J. Microbiol. Biotechnol. VL - 28 IS - 11 N2 - Malassezia pachydermatis is a commensal yeast found on the skin of dogs. However, M. pachydermatis is also considered an opportunistic pathogen and is associated with various canine skin diseases including otitis externa and atopic dermatitis, which usually require treatment using an azole antifungal drug, such as ketoconazole. In this study, we isolated a ketoconazole-resistant strain of M. pachydermatis, designated "KCTC 27587," from the external ear canal of a dog with otitis externa and analyzed its resistance mechanism. To understand the mechanism underlying ketoconazole resistance of the clinical isolate M. pachydermatis KCTC 27587, the whole genome of the yeast was sequenced using the PacBio platform and was compared with M. pachydermatis type strain CBS 1879. We found that a ~84-kb region in chromosome 4 of M. pachydermatis KCTC 27587 was tandemly quadruplicated. The quadruplicated region contains 52 protein coding genes, including the homologs of ERG4 and ERG11, whose overexpression is known to be associated with azole resistance. Our data suggest that the quadruplication of the ~84-kb region may be the cause of the ketoconazole resistance in M. pachydermatis KCTC 27587. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/30562885/Genomic_Tandem_Quadruplication_is_Associated_with_Ketoconazole_Resistance_in_Malassezia_pachydermatis_ L2 - http://www.jmb.or.kr/journal/viewJournal.html?doi=10.4014/jmb.1810.10019 DB - PRIME DP - Unbound Medicine ER -