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Use of a monoclonal antibody to detect Pneumocystis carinii in induced sputum and bronchoalveolar lavage fluid by immunoperoxidase staining.
Arch Pathol Lab Med. 1988 Dec; 112(12):1233-6.AP

Abstract

We evaluated the utility of a monoclonal antibody that recognizes Pneumocystis carinii as a diagnostic tool in specimens of bronchoalveolar lavage fluid and sputum from patients in whom a diagnosis of P carinii pneumonia was being considered. In addition to routine processing for diagnosis by morphologic recognition of P carinii on a Diff-Quik-stained specimen, the specimen was reacted with a monoclonal antibody to P carinii and visualized in an avidin-biotin horseradish peroxidase technique. Of 50 specimens evaluated, there was 94% agreement between results of conventional Diff-Quik staining and immunoperoxidase staining. Two Diff-Quik-positive specimens were negative by immunoperoxidase staining, and one Diff-Quik-negative specimen was positive by immunoperoxidase staining. These discrepancies are most likely attributable to random distribution of P carinii onto smears. The monoclonal antibody used in this study can accurately detect P carinii in induced sputum and bronchoalveolar lavage fluid specimens.

Authors+Show Affiliations

Department of Laboratory Medicine, Veterans Administration Medical Center, San Francisco.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

3056320

Citation

Blumenfeld, W, and J A. Kovacs. "Use of a Monoclonal Antibody to Detect Pneumocystis Carinii in Induced Sputum and Bronchoalveolar Lavage Fluid By Immunoperoxidase Staining." Archives of Pathology & Laboratory Medicine, vol. 112, no. 12, 1988, pp. 1233-6.
Blumenfeld W, Kovacs JA. Use of a monoclonal antibody to detect Pneumocystis carinii in induced sputum and bronchoalveolar lavage fluid by immunoperoxidase staining. Arch Pathol Lab Med. 1988;112(12):1233-6.
Blumenfeld, W., & Kovacs, J. A. (1988). Use of a monoclonal antibody to detect Pneumocystis carinii in induced sputum and bronchoalveolar lavage fluid by immunoperoxidase staining. Archives of Pathology & Laboratory Medicine, 112(12), 1233-6.
Blumenfeld W, Kovacs JA. Use of a Monoclonal Antibody to Detect Pneumocystis Carinii in Induced Sputum and Bronchoalveolar Lavage Fluid By Immunoperoxidase Staining. Arch Pathol Lab Med. 1988;112(12):1233-6. PubMed PMID: 3056320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of a monoclonal antibody to detect Pneumocystis carinii in induced sputum and bronchoalveolar lavage fluid by immunoperoxidase staining. AU - Blumenfeld,W, AU - Kovacs,J A, PY - 1988/12/1/pubmed PY - 1988/12/1/medline PY - 1988/12/1/entrez SP - 1233 EP - 6 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 112 IS - 12 N2 - We evaluated the utility of a monoclonal antibody that recognizes Pneumocystis carinii as a diagnostic tool in specimens of bronchoalveolar lavage fluid and sputum from patients in whom a diagnosis of P carinii pneumonia was being considered. In addition to routine processing for diagnosis by morphologic recognition of P carinii on a Diff-Quik-stained specimen, the specimen was reacted with a monoclonal antibody to P carinii and visualized in an avidin-biotin horseradish peroxidase technique. Of 50 specimens evaluated, there was 94% agreement between results of conventional Diff-Quik staining and immunoperoxidase staining. Two Diff-Quik-positive specimens were negative by immunoperoxidase staining, and one Diff-Quik-negative specimen was positive by immunoperoxidase staining. These discrepancies are most likely attributable to random distribution of P carinii onto smears. The monoclonal antibody used in this study can accurately detect P carinii in induced sputum and bronchoalveolar lavage fluid specimens. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/3056320/Use_of_a_monoclonal_antibody_to_detect_Pneumocystis_carinii_in_induced_sputum_and_bronchoalveolar_lavage_fluid_by_immunoperoxidase_staining_ L2 - https://medlineplus.gov/hivaids.html DB - PRIME DP - Unbound Medicine ER -