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Anti-melanogenesis properties of condensed tannins from Vigna angularis seeds with potent antioxidant and DNA damage protection activities.
Food Funct. 2019 Jan 22; 10(1):99-111.FF

Abstract

Condensed tannins contained in food are known to have many beneficial impacts on human health. In this study, we attempt to evaluate the structural features, antityrosinase effects, anti-melanogenesis properties, antioxidant activity and DNA damage protection activity of condensed tannins purified from the seeds of Vigna angularis (Willd.) Ohwi et Ohashi. MALDI-TOF MS, ESI-Full-MS, and HPLC-ESI-MS demonstrated that condensed tannins are composed of procyanidins, prodelphinidins and their gallates, among which procyanidins are the dominant components. As reversible and mixed-type inhibitors of tyrosinase, condensed tannins from V. angularis strongly inhibited the monophenolase and odiphenolase activities with IC50 values of 130.0 ± 0.5 and 35.1 ± 2.0 μg mL-1, respectively. What's more, condensed tannins had a good inhibitory effect on cell proliferation, cellular tyrosinase activity, and melanogenesis of B16 mouse melanoma cells. Based on fluorescence quenching analyses, these compounds were determined to be effective quenchers of the enzyme and its substrates. According to molecular docking, the strong interaction between condensed tannins and tyrosinase was mainly driven by hydrogen bonding and hydrophobic force. In addition, condensed tannins showed a powerful antioxidant capacity and DNA damage protection activity. Therefore, condensed tannins from V. angularis have feasible applications in food, medicine, and the cosmetics industry.

Authors+Show Affiliations

College of Life Science and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, Jiangxi 330022, People's Republic of China. chaiweiming@jxnu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30565612

Citation

Chai, Wei-Ming, et al. "Anti-melanogenesis Properties of Condensed Tannins From Vigna Angularis Seeds With Potent Antioxidant and DNA Damage Protection Activities." Food & Function, vol. 10, no. 1, 2019, pp. 99-111.
Chai WM, Wei QM, Deng WL, et al. Anti-melanogenesis properties of condensed tannins from Vigna angularis seeds with potent antioxidant and DNA damage protection activities. Food Funct. 2019;10(1):99-111.
Chai, W. M., Wei, Q. M., Deng, W. L., Zheng, Y. L., Chen, X. Y., Huang, Q., Ou-Yang, C., & Peng, Y. Y. (2019). Anti-melanogenesis properties of condensed tannins from Vigna angularis seeds with potent antioxidant and DNA damage protection activities. Food & Function, 10(1), 99-111. https://doi.org/10.1039/c8fo01979g
Chai WM, et al. Anti-melanogenesis Properties of Condensed Tannins From Vigna Angularis Seeds With Potent Antioxidant and DNA Damage Protection Activities. Food Funct. 2019 Jan 22;10(1):99-111. PubMed PMID: 30565612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-melanogenesis properties of condensed tannins from Vigna angularis seeds with potent antioxidant and DNA damage protection activities. AU - Chai,Wei-Ming, AU - Wei,Qi-Ming, AU - Deng,Wei-Liang, AU - Zheng,Yun-Ling, AU - Chen,Xiao-Ying, AU - Huang,Qian, AU - Ou-Yang,Chong, AU - Peng,Yi-Yuan, PY - 2018/12/20/pubmed PY - 2019/4/10/medline PY - 2018/12/20/entrez SP - 99 EP - 111 JF - Food & function JO - Food Funct VL - 10 IS - 1 N2 - Condensed tannins contained in food are known to have many beneficial impacts on human health. In this study, we attempt to evaluate the structural features, antityrosinase effects, anti-melanogenesis properties, antioxidant activity and DNA damage protection activity of condensed tannins purified from the seeds of Vigna angularis (Willd.) Ohwi et Ohashi. MALDI-TOF MS, ESI-Full-MS, and HPLC-ESI-MS demonstrated that condensed tannins are composed of procyanidins, prodelphinidins and their gallates, among which procyanidins are the dominant components. As reversible and mixed-type inhibitors of tyrosinase, condensed tannins from V. angularis strongly inhibited the monophenolase and odiphenolase activities with IC50 values of 130.0 ± 0.5 and 35.1 ± 2.0 μg mL-1, respectively. What's more, condensed tannins had a good inhibitory effect on cell proliferation, cellular tyrosinase activity, and melanogenesis of B16 mouse melanoma cells. Based on fluorescence quenching analyses, these compounds were determined to be effective quenchers of the enzyme and its substrates. According to molecular docking, the strong interaction between condensed tannins and tyrosinase was mainly driven by hydrogen bonding and hydrophobic force. In addition, condensed tannins showed a powerful antioxidant capacity and DNA damage protection activity. Therefore, condensed tannins from V. angularis have feasible applications in food, medicine, and the cosmetics industry. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/30565612/Anti_melanogenesis_properties_of_condensed_tannins_from_Vigna_angularis_seeds_with_potent_antioxidant_and_DNA_damage_protection_activities_ DB - PRIME DP - Unbound Medicine ER -