TY - JOUR T1 - Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. AU - Li,Xiaowei, AU - Ke,Yuebin, AU - Wang,Yingyu, AU - Wang,Chengfei, AU - Ye,Dongyang, AU - Hu,Xue, AU - Zhou,Lan, AU - Xia,Xi, Y1 - 2018/12/18/ PY - 2018/11/30/received PY - 2018/12/11/revised PY - 2018/12/15/accepted PY - 2018/12/21/entrez PY - 2018/12/21/pubmed PY - 2019/3/21/medline KW - d-SPE KW - honey KW - liquid chromatography KW - mass spectrometry KW - nitroimidazole JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 12 N2 - An ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles and metabolites were isolated and enriched by dispersive-solid phase extraction using mixed-mode strong cation-exchange sorbent. The determination involves separation of analytes on an UHPLC C18 column and detection by multiple reaction monitoring in positive ionization mode. The recovery of the method was ranged from 90.2 to 105.6% with inter-day relative standard deviations of less than 11.2%. The limits of detection and limits of quantification were in the ranges of 0.02[-]0.07 µg/kg and 0.05[-]0.2 µg/kg, respectively. Honey samples from the market were analyzed to demonstrate the applicability of the proposed method. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30567334/Confirmatory_Analysis_of_Nitroimidazoles_and_Hydroxy_Metabolites_in_Honey_by_Dispersive_Solid_Phase_Extraction_and_Ultra_High_Performance_Liquid_Chromatography_Tandem_Mass_Spectrometry_ DB - PRIME DP - Unbound Medicine ER -