Target-driven switch-on fluorescence aptasensor for trace aflatoxin B1 determination based on highly fluorescent ternary CdZnTe quantum dots.Anal Chim Acta. 2019 Jan 24; 1047:163-171.AC
Development of sensitive methods for trace aflatoxin B1 (AFB1) determination is of great significance due to its high toxicity and carcinogenicity. Herein, 3-mercaptopropionic acid (MPA)-capped ternary CdZnTe quantum dots (QDs) have been prepared via a simple hydrothermal route. We found that they exhibited enhanced intensity when benchmarked against their binary counterpart CdTe QDs. On this basis, a target-driven switch-on fluorescence aptasensor for trace AFB1 determination has been developed by employing the fluorescence resonance energy transfer (FRET) between the CdZnTe QDs and Au nanoparticles (AuNPs) pair. In the detection diagram, amino group-functionalized aptamers against AFB1 were firstly labelled with the CdZnTe QDs donors coated on silica nanospheres while the AuNPs acceptors were bioconjugated with the thiol group-modified complementary DNA (cDNA) of aptamer. By taking advantage of the DNA hybridization of aptamer and cDNA, the CdZnTe QDs (energy donor) and AuNPs (energy acceptor) were brought into close proximity, thereby leading to the occurrence of FRET during the aptasensor fabrication. When the aptasensor was incubated with AFB1, the specific binding between aptamer and target resulted in the detachment of AuNPs acceptors. This behavior would disturb the FRET process and led to the subsequent fluorescence recovery of CdZnTe QDs. Such designed aptasensor showed an increased fluorescence recovery upon the increasing concentration of AFB1 over a broad range of 50 pg mL-1 - 100 ng mL-1 and succeeded in spiked peanut samples. The proposed aptasensor is separation-free and easy-to-use, which might open up new possibilities in aptasensor fabrication by employing the novel CdZnTe QDs-AuNPs pair.
