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Developmental toxicity of the triazole fungicide cyproconazole in embryo-larval stages of zebrafish (Danio rerio).
Environ Sci Pollut Res Int 2019; 26(5):4913-4923ES

Abstract

Cyproconazole is a triazole fungicide used to protect a diverse range of fruits, vegetables, and grain crops. As such, it has the potential to enter aquatic environments and affect non-target organisms. The objective of this study was to assess the acute toxicity of the triazole fungicide cyproconazole to zebrafish embryos by assessing mortality, developmental defects, morphological abnormality, oxidative respiration, and locomotor activity following a 96-h exposure. Zebrafish embryos at 6-h post-fertilization (hpf) were exposed to either a solvent control (0.1% DMSO, v/v), or one dose of 10, 25, 50, 100, 250, and 500 μM cyproconazole for 96 h. Data indicated that cyproconazole exhibited low toxicity to zebrafish embryos, with a 96-h LC50 value of 90.6 μM (~ 26.4 mg/L). Zebrafish embryos/larvae displayed a significant decrease in spontaneous movement, hatching rate, and heartbeats/20 s with 50, 100, and 250 μM cyproconazole exposure. Malformations (i.e., pericardial edema, yolk sac edema, tail deformation, and spine deformation) were also detected in zebrafish exposed to ≥ 50 μM cyproconazole, with significant increases in cumulative deformity rate at 48, 72, and 96 hpf. In addition, a 20-30% decrease in basal and oligomycin-induced ATP respiration was observed after 24-h exposure to 500 μM cyproconazole in embryos. To determine if cyproconazole affected locomotor activity, a dark photokinesis assay was conducted in larvae following 7-day exposure to 1, 10, and 25 μM cyproconazole in two independent trials. Activity in the dark period was decreased for zebrafish exposed to 25 μM cyproconazole in the first trial, and hypoactivity was also observed in zebrafish exposed to 1 μM cyproconazole in a second trial, suggesting that cyproconazole can affect locomotor activity. These data improve understanding of the toxicity of cyproconazole in developing zebrafish and contribute to environmental risk assessments for the triazole fungicides on aquatic organisms. We report that, based on the overall endpoints assessed, cyproconazole exhibits low risk for developing fish embryos, as many effects were observed above environmentally-relevant levels.

Authors+Show Affiliations

Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China. Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA.Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China. Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA.Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China.Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA. cmartyn@ufl.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30569354

Citation

Cao, Fangjie, et al. "Developmental Toxicity of the Triazole Fungicide Cyproconazole in Embryo-larval Stages of Zebrafish (Danio Rerio)." Environmental Science and Pollution Research International, vol. 26, no. 5, 2019, pp. 4913-4923.
Cao F, Souders CL, Li P, et al. Developmental toxicity of the triazole fungicide cyproconazole in embryo-larval stages of zebrafish (Danio rerio). Environ Sci Pollut Res Int. 2019;26(5):4913-4923.
Cao, F., Souders, C. L., Li, P., Pang, S., Qiu, L., & Martyniuk, C. J. (2019). Developmental toxicity of the triazole fungicide cyproconazole in embryo-larval stages of zebrafish (Danio rerio). Environmental Science and Pollution Research International, 26(5), pp. 4913-4923. doi:10.1007/s11356-018-3957-z.
Cao F, et al. Developmental Toxicity of the Triazole Fungicide Cyproconazole in Embryo-larval Stages of Zebrafish (Danio Rerio). Environ Sci Pollut Res Int. 2019;26(5):4913-4923. PubMed PMID: 30569354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental toxicity of the triazole fungicide cyproconazole in embryo-larval stages of zebrafish (Danio rerio). AU - Cao,Fangjie, AU - Souders,Christopher L,2nd AU - Li,Pengfei, AU - Pang,Sen, AU - Qiu,Lihong, AU - Martyniuk,Christopher J, Y1 - 2018/12/19/ PY - 2018/08/24/received PY - 2018/12/07/accepted PY - 2018/12/21/pubmed PY - 2019/3/30/medline PY - 2018/12/21/entrez KW - Cyproconazole KW - Developmental defects KW - Locomotor activity KW - Malformations KW - Oxidative respiration KW - Zebrafish embryos SP - 4913 EP - 4923 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 26 IS - 5 N2 - Cyproconazole is a triazole fungicide used to protect a diverse range of fruits, vegetables, and grain crops. As such, it has the potential to enter aquatic environments and affect non-target organisms. The objective of this study was to assess the acute toxicity of the triazole fungicide cyproconazole to zebrafish embryos by assessing mortality, developmental defects, morphological abnormality, oxidative respiration, and locomotor activity following a 96-h exposure. Zebrafish embryos at 6-h post-fertilization (hpf) were exposed to either a solvent control (0.1% DMSO, v/v), or one dose of 10, 25, 50, 100, 250, and 500 μM cyproconazole for 96 h. Data indicated that cyproconazole exhibited low toxicity to zebrafish embryos, with a 96-h LC50 value of 90.6 μM (~ 26.4 mg/L). Zebrafish embryos/larvae displayed a significant decrease in spontaneous movement, hatching rate, and heartbeats/20 s with 50, 100, and 250 μM cyproconazole exposure. Malformations (i.e., pericardial edema, yolk sac edema, tail deformation, and spine deformation) were also detected in zebrafish exposed to ≥ 50 μM cyproconazole, with significant increases in cumulative deformity rate at 48, 72, and 96 hpf. In addition, a 20-30% decrease in basal and oligomycin-induced ATP respiration was observed after 24-h exposure to 500 μM cyproconazole in embryos. To determine if cyproconazole affected locomotor activity, a dark photokinesis assay was conducted in larvae following 7-day exposure to 1, 10, and 25 μM cyproconazole in two independent trials. Activity in the dark period was decreased for zebrafish exposed to 25 μM cyproconazole in the first trial, and hypoactivity was also observed in zebrafish exposed to 1 μM cyproconazole in a second trial, suggesting that cyproconazole can affect locomotor activity. These data improve understanding of the toxicity of cyproconazole in developing zebrafish and contribute to environmental risk assessments for the triazole fungicides on aquatic organisms. We report that, based on the overall endpoints assessed, cyproconazole exhibits low risk for developing fish embryos, as many effects were observed above environmentally-relevant levels. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/30569354/Developmental_toxicity_of_the_triazole_fungicide_cyproconazole_in_embryo_larval_stages_of_zebrafish__Danio_rerio__ L2 - https://dx.doi.org/10.1007/s11356-018-3957-z DB - PRIME DP - Unbound Medicine ER -