Tags

Type your tag names separated by a space and hit enter

HPLC-MS/MS chemical characterization and biological properties of Origanum onites extracts: a recent insight.
Int J Environ Health Res. 2019 Dec; 29(6):607-621.IJ

Abstract

This study investigated into the phytochemical profile and biological properties of extracts (methanol and aqueous) of Origanum onites based on the antioxidant, enzyme inhibitory, and antibacterial activities. The aqueous extract exhibited higher antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), ferric reducing antioxidant power, cupric reducing antioxidant capacity, phosphomolybdenum, and metal chelating assays, compared to the methanol extract. In contrast, the methanol extract was the most effective inhibitor of acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase. The methanol extract also showed higher antibacterial activity with highest inhibition against Escherichia coli (MIC = 6.25 mg/mL). The total phenolic content was higher in the aqueous extract while the methanol extract possessed higher total flavonoid content. A total of 28 and 18 compounds (belonging to polyphenols, flavonoids, terpenoids, and ester classes) were identified from the methanol and water extracts, respectively. These findings suggest that O. onites could be helpful in the management of oxidative stress-associated diseases including diabetes and neurodegenerative complications. Abbreviations: ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid; ACAE: acarbose equivalent; AChE: acetylcholinesterase; AD: Alzheimer's disease; BChE: butyrylcholinesterase; CUPRAC: cupric reducing antioxidant capacity; DPPH: 1,1-diphenyl-2-picrylhydrazyl; EDTAE: EDTA equivalent; FRAP: ferric reducing antioxidant power; GAE: gallic acid equivalent; GALAE: galatamine equivalent; HPLC: high performance liquid chromatography; KAE: kojic acid equivalent; RE: rutin equivalents; TE: trolox equivalent; TPC: total phenolic content; TFC: total flavonoid content.

Authors+Show Affiliations

Department of Health Sciences, Faculty of Science, University of Mauritius , Réduit , Mauritius.Department of Biology, Science Faculty, Selcuk University , Konya , Turkey.Department of Medicinal Laboratory, Vocational School of Health Services, Selcuk University , Konya , Turkey.Department of Biology, Science Faculty, Selcuk University , Konya , Turkey.Department of Analytical Chemistry, Pavol Jozef Šafárik University in Košice , Košice , Slovakia.Agricultural and Molecular Research and Service Institute, University of Nyíregyháza , Nyíregyháza , Hungary.Agricultural and Molecular Research and Service Institute, University of Nyíregyháza , Nyíregyháza , Hungary.Department of Health Sciences, Faculty of Science, University of Mauritius , Réduit , Mauritius.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30569760

Citation

Mahomoodally, Mohamad Fawzi, et al. "HPLC-MS/MS Chemical Characterization and Biological Properties of Origanum Onites Extracts: a Recent Insight." International Journal of Environmental Health Research, vol. 29, no. 6, 2019, pp. 607-621.
Mahomoodally MF, Zengin G, Aladag MO, et al. HPLC-MS/MS chemical characterization and biological properties of Origanum onites extracts: a recent insight. Int J Environ Health Res. 2019;29(6):607-621.
Mahomoodally, M. F., Zengin, G., Aladag, M. O., Ozparlak, H., Diuzheva, A., Jekő, J., Cziáky, Z., & Aumeeruddy, M. Z. (2019). HPLC-MS/MS chemical characterization and biological properties of Origanum onites extracts: a recent insight. International Journal of Environmental Health Research, 29(6), 607-621. https://doi.org/10.1080/09603123.2018.1558184
Mahomoodally MF, et al. HPLC-MS/MS Chemical Characterization and Biological Properties of Origanum Onites Extracts: a Recent Insight. Int J Environ Health Res. 2019;29(6):607-621. PubMed PMID: 30569760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC-MS/MS chemical characterization and biological properties of Origanum onites extracts: a recent insight. AU - Mahomoodally,Mohamad Fawzi, AU - Zengin,Gokhan, AU - Aladag,Mustafa Onur, AU - Ozparlak,Haluk, AU - Diuzheva,Alina, AU - Jekő,József, AU - Cziáky,Zoltán, AU - Aumeeruddy,Muhammad Zakariyyah, Y1 - 2018/12/20/ PY - 2018/12/21/pubmed PY - 2019/12/21/medline PY - 2018/12/21/entrez KW - KW - HPLC-MS/MS KW - antioxidant KW - bio-active compounds KW - enzyme inhibition SP - 607 EP - 621 JF - International journal of environmental health research JO - Int J Environ Health Res VL - 29 IS - 6 N2 - This study investigated into the phytochemical profile and biological properties of extracts (methanol and aqueous) of Origanum onites based on the antioxidant, enzyme inhibitory, and antibacterial activities. The aqueous extract exhibited higher antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), ferric reducing antioxidant power, cupric reducing antioxidant capacity, phosphomolybdenum, and metal chelating assays, compared to the methanol extract. In contrast, the methanol extract was the most effective inhibitor of acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase. The methanol extract also showed higher antibacterial activity with highest inhibition against Escherichia coli (MIC = 6.25 mg/mL). The total phenolic content was higher in the aqueous extract while the methanol extract possessed higher total flavonoid content. A total of 28 and 18 compounds (belonging to polyphenols, flavonoids, terpenoids, and ester classes) were identified from the methanol and water extracts, respectively. These findings suggest that O. onites could be helpful in the management of oxidative stress-associated diseases including diabetes and neurodegenerative complications. Abbreviations: ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid; ACAE: acarbose equivalent; AChE: acetylcholinesterase; AD: Alzheimer's disease; BChE: butyrylcholinesterase; CUPRAC: cupric reducing antioxidant capacity; DPPH: 1,1-diphenyl-2-picrylhydrazyl; EDTAE: EDTA equivalent; FRAP: ferric reducing antioxidant power; GAE: gallic acid equivalent; GALAE: galatamine equivalent; HPLC: high performance liquid chromatography; KAE: kojic acid equivalent; RE: rutin equivalents; TE: trolox equivalent; TPC: total phenolic content; TFC: total flavonoid content. SN - 1369-1619 UR - https://www.unboundmedicine.com/medline/citation/30569760/HPLC_MS/MS_chemical_characterization_and_biological_properties_of_Origanum_onites_extracts:_a_recent_insight_ L2 - https://www.tandfonline.com/doi/full/10.1080/09603123.2018.1558184 DB - PRIME DP - Unbound Medicine ER -