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Aspirin-triggered resolvin D1 alleviates paraquat-induced acute lung injury in mice.
Life Sci. 2019 Feb 01; 218:38-46.LS

Abstract

AIMS

In the present study, we aimed to evaluate the role of aspirin-triggered resolvin D1 (AT-RvD1) in paraquat (PQ)-induced acute lung injury (ALI) in mice.

MAIN METHODS

We used C57BL/6J mice as experimental subjects to establish mouse models of ALI via intraperitoneal (IP) injection of PQ (28 mg/kg). The mice were then administered AT-RvD1 (10 or 100 ng) via the tail vein 2 h after exposure to PQ and were sacrificed at 72 h post exposure to harvest bronchoalveolar lavage fluid (BALF), blood and lung tissue samples. The samples were used to evaluate the histopathological changes, inflammation reaction and oxidative stress in the lung tissues.

KEY FINDINGS

Compared with those of the PQ group, the administration of AT-RvD1 significantly (1) alleviated the histopathological changes in the lung tissues; (2) reduced the lung W/D weight ratio and the total protein content in the BALF; (3) activated nuclear factor erythroid-2 related factor 2 (Nrf2) and up-regulated the expression of its downstream genes (NADPH: quinone oxidoreductase-1, NQO1 and heme oxygenase-1, HO-1); (4) reduced the malondialdehyde(MDA) level in the lung tissues; (5) reduced the total cell, neutrophil, and macrophage counts in the BALF; (6) reduced the myeloperoxidase (MPO) activity in the lung tissues; (7) reduced the percent of Ly-6G+ CD41+ cells in the peripheral blood; (8) inhibited the activation of nuclear factor-κB (NF-κB) and the expression of P-selectin; and (9) reduced interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in the BALF.

SIGNIFICANCE

Administration of AT-RvD1 can effectively inhibit PQ-induced oxidative stress injury, inflammatory responses, and pulmonary edema, thereby alleviating PQ-induced ALI.

Authors+Show Affiliations

Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China. Electronic address: zhaom@sj-hospital.org.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30571954

Citation

Hu, Xiao, et al. "Aspirin-triggered Resolvin D1 Alleviates Paraquat-induced Acute Lung Injury in Mice." Life Sciences, vol. 218, 2019, pp. 38-46.
Hu X, Shen H, Wang Y, et al. Aspirin-triggered resolvin D1 alleviates paraquat-induced acute lung injury in mice. Life Sci. 2019;218:38-46.
Hu, X., Shen, H., Wang, Y., Zhang, L., & Zhao, M. (2019). Aspirin-triggered resolvin D1 alleviates paraquat-induced acute lung injury in mice. Life Sciences, 218, 38-46. https://doi.org/10.1016/j.lfs.2018.12.028
Hu X, et al. Aspirin-triggered Resolvin D1 Alleviates Paraquat-induced Acute Lung Injury in Mice. Life Sci. 2019 Feb 1;218:38-46. PubMed PMID: 30571954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aspirin-triggered resolvin D1 alleviates paraquat-induced acute lung injury in mice. AU - Hu,Xiao, AU - Shen,Haitao, AU - Wang,Yu, AU - Zhang,Lichun, AU - Zhao,Min, Y1 - 2018/12/17/ PY - 2018/10/29/received PY - 2018/12/07/revised PY - 2018/12/15/accepted PY - 2018/12/21/pubmed PY - 2019/3/2/medline PY - 2018/12/21/entrez KW - AT-RvD1 KW - Acute lung injury KW - Paraquat SP - 38 EP - 46 JF - Life sciences JO - Life Sci VL - 218 N2 - AIMS: In the present study, we aimed to evaluate the role of aspirin-triggered resolvin D1 (AT-RvD1) in paraquat (PQ)-induced acute lung injury (ALI) in mice. MAIN METHODS: We used C57BL/6J mice as experimental subjects to establish mouse models of ALI via intraperitoneal (IP) injection of PQ (28 mg/kg). The mice were then administered AT-RvD1 (10 or 100 ng) via the tail vein 2 h after exposure to PQ and were sacrificed at 72 h post exposure to harvest bronchoalveolar lavage fluid (BALF), blood and lung tissue samples. The samples were used to evaluate the histopathological changes, inflammation reaction and oxidative stress in the lung tissues. KEY FINDINGS: Compared with those of the PQ group, the administration of AT-RvD1 significantly (1) alleviated the histopathological changes in the lung tissues; (2) reduced the lung W/D weight ratio and the total protein content in the BALF; (3) activated nuclear factor erythroid-2 related factor 2 (Nrf2) and up-regulated the expression of its downstream genes (NADPH: quinone oxidoreductase-1, NQO1 and heme oxygenase-1, HO-1); (4) reduced the malondialdehyde(MDA) level in the lung tissues; (5) reduced the total cell, neutrophil, and macrophage counts in the BALF; (6) reduced the myeloperoxidase (MPO) activity in the lung tissues; (7) reduced the percent of Ly-6G+ CD41+ cells in the peripheral blood; (8) inhibited the activation of nuclear factor-κB (NF-κB) and the expression of P-selectin; and (9) reduced interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in the BALF. SIGNIFICANCE: Administration of AT-RvD1 can effectively inhibit PQ-induced oxidative stress injury, inflammatory responses, and pulmonary edema, thereby alleviating PQ-induced ALI. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/30571954/Aspirin_triggered_resolvin_D1_alleviates_paraquat_induced_acute_lung_injury_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(18)30821-X DB - PRIME DP - Unbound Medicine ER -