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Molecular characterization of novel sequence type of carbapenem-resistant New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae in the neonatal intensive care unit of an Indian hospital.
Int J Antimicrob Agents 2019; 53(4):525-529IJ

Abstract

Emergence of multi-drug resistance, especially carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major threat to public health. The aim of this study was to characterize CRKP isolates from infants admitted to the Neonatal Intensive Care Unit (NICU) to find the clonal outbreak of New Delhi metallo-β-lactamase (NDM) producers. In this study 17 CRKP isolates were analysed. Antimicrobial susceptibility of the isolates was determined by the disc diffusion and micro-dilution method. Carba-NP test and double-disk synergy test (DDST) were performed for the detection of carbapenemase and metallo-β-lactamase-producing K. pneumoniae. Antibiotic-resistant markers were detected by polymerase chain reaction (PCR) followed by sequencing. Clonal relatedness of the isolates was checked by multi-locus sequence typing. Conjugation experiments were performed to determine the transferability of the plasmids. All 17 CKRP isolates were found to carry blaNDM (13 blaNDM-1, 1 blaNDM-4 and 3 blaNDM-5), seven isolates carried blaOXA-48, 13 isolates had blaCTX-M-15, seven isolates carried blaCMY-1 and five isolates were found to carry blaSHV-1 on conjugative plasmids of different types (IncFIA, IncFIB, IncFIIAs, IncFIC, IncA/C, IncF, IncK, IncX, IncW and IncY). Of six different sequence types (STs) identified, ST3344 was detected as a novel ST in two K. pneumoniae isolates. Genetic environment analysis revealed ISAba125 and bleomycin genes flanking to all blaNDM variants. This is the first report of novel ST3344 in two NDM-1-producing K. pneumoniae isolates from neonates admitted to the NICU of a North Indian Hospital. This study is provides understanding of the genetic features of this newly emerged strain type.

Authors+Show Affiliations

Medical Microbiology and Molecular Biology Lab., Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.Pediatrics Department, JN Medical College and Hospital, AMU Aligarh, India.Medical Microbiology and Molecular Biology Lab., Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. Electronic address: asadukhan72@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30578964

Citation

Ahmad, Nayeem, et al. "Molecular Characterization of Novel Sequence Type of Carbapenem-resistant New Delhi Metallo-β-lactamase-1-producing Klebsiella Pneumoniae in the Neonatal Intensive Care Unit of an Indian Hospital." International Journal of Antimicrobial Agents, vol. 53, no. 4, 2019, pp. 525-529.
Ahmad N, Ali SM, Khan AU. Molecular characterization of novel sequence type of carbapenem-resistant New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae in the neonatal intensive care unit of an Indian hospital. Int J Antimicrob Agents. 2019;53(4):525-529.
Ahmad, N., Ali, S. M., & Khan, A. U. (2019). Molecular characterization of novel sequence type of carbapenem-resistant New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae in the neonatal intensive care unit of an Indian hospital. International Journal of Antimicrobial Agents, 53(4), pp. 525-529. doi:10.1016/j.ijantimicag.2018.12.005.
Ahmad N, Ali SM, Khan AU. Molecular Characterization of Novel Sequence Type of Carbapenem-resistant New Delhi Metallo-β-lactamase-1-producing Klebsiella Pneumoniae in the Neonatal Intensive Care Unit of an Indian Hospital. Int J Antimicrob Agents. 2019;53(4):525-529. PubMed PMID: 30578964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of novel sequence type of carbapenem-resistant New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae in the neonatal intensive care unit of an Indian hospital. AU - Ahmad,Nayeem, AU - Ali,Syed M, AU - Khan,Asad U, Y1 - 2018/12/20/ PY - 2018/09/26/received PY - 2018/12/06/revised PY - 2018/12/15/accepted PY - 2018/12/24/pubmed PY - 2018/12/24/medline PY - 2018/12/23/entrez KW - Carbapenem-resistant KW - Klebsiella pneumoniae KW - MLST KW - NDM KW - NICU KW - OXA-48 SP - 525 EP - 529 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 53 IS - 4 N2 - Emergence of multi-drug resistance, especially carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major threat to public health. The aim of this study was to characterize CRKP isolates from infants admitted to the Neonatal Intensive Care Unit (NICU) to find the clonal outbreak of New Delhi metallo-β-lactamase (NDM) producers. In this study 17 CRKP isolates were analysed. Antimicrobial susceptibility of the isolates was determined by the disc diffusion and micro-dilution method. Carba-NP test and double-disk synergy test (DDST) were performed for the detection of carbapenemase and metallo-β-lactamase-producing K. pneumoniae. Antibiotic-resistant markers were detected by polymerase chain reaction (PCR) followed by sequencing. Clonal relatedness of the isolates was checked by multi-locus sequence typing. Conjugation experiments were performed to determine the transferability of the plasmids. All 17 CKRP isolates were found to carry blaNDM (13 blaNDM-1, 1 blaNDM-4 and 3 blaNDM-5), seven isolates carried blaOXA-48, 13 isolates had blaCTX-M-15, seven isolates carried blaCMY-1 and five isolates were found to carry blaSHV-1 on conjugative plasmids of different types (IncFIA, IncFIB, IncFIIAs, IncFIC, IncA/C, IncF, IncK, IncX, IncW and IncY). Of six different sequence types (STs) identified, ST3344 was detected as a novel ST in two K. pneumoniae isolates. Genetic environment analysis revealed ISAba125 and bleomycin genes flanking to all blaNDM variants. This is the first report of novel ST3344 in two NDM-1-producing K. pneumoniae isolates from neonates admitted to the NICU of a North Indian Hospital. This study is provides understanding of the genetic features of this newly emerged strain type. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/30578964/Molecular_characterization_of_novel_sequence_type_of_carbapenem_resistant_New_Delhi_metallo_β_lactamase_1_producing_Klebsiella_pneumoniae_in_the_neonatal_intensive_care_unit_of_an_Indian_hospital_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(18)30367-4 DB - PRIME DP - Unbound Medicine ER -