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Simultaneous determination of vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices using liquid chromatography-tandem mass spectrometry.
J Pharm Biomed Anal 2019; 165:315-324JP

Abstract

Vancomycin (VCM) is a glycopeptide antibiotic, widely used against methicillin-resistant Staphylococcus aureus infections, and its clearance is highly correlated to creatinine (CRE) clearance. Usually, VCM and CRE levels are measured in serum or plasma specimens obtained from venous blood, after phlebotomy. As the majority of hospitals in Developing Countries do not have on-site access to VCM measurements, the availability of data on patient's VCM levels would involve the transport of liquid samples to specialized laboratories. An alternative to overcome the logistic difficulties of liquid biological specimens is the use of dried microsamples, such as plasma applied to volumetric absorptive microsampling (VAMS) devices. The aim of this study was to develop and validate a LC-MS/MS assay for the simultaneous determination of VCM and CRE in plasma applied to VAMS devices. VCM and CRE levels were determined in clinical relevant ranges with acceptable precision and accuracy and both compounds were stable in VAMS for up to two weeks at 45 °C. Concentrations measured in VAMS differed from those measured in plasma and the use of estimation approaches was necessary to estimate plasma concentrations. VCM levels in plasma can be estimated by multiplying VAMS measurements by the correction factor of 0.934. The unbiased estimation of CRE plasma levels from VAMS required the use of the equation CREplasma = 0.9808 * CREVAMS + 1.6621. VAMS can be used as an alternative for short-term storage and transportation of plasma at ambient and high temperatures, allowing a more widespread access to VCM therapeutic drug monitoring in limited-resources settings.

Authors+Show Affiliations

Laboratory of Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil; Postgraduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil.Laboratory of Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil.Universidade de Passo Fundo, Passo Fundo, Brazil.Hospital São Vicente de Paulo, Passo Fundo, Brazil.Laboratory of Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil; Postgraduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil.Laboratory of Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil; Postgraduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, Brazil. Electronic address: rafael.linden@feevale.br.

Pub Type(s)

Journal Article
Validation Studies

Language

eng

PubMed ID

30579232

Citation

Andriguetti, Nadine B., et al. "Simultaneous Determination of Vancomycin and Creatinine in Plasma Applied to Volumetric Absorptive Microsampling Devices Using Liquid Chromatography-tandem Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 165, 2019, pp. 315-324.
Andriguetti NB, Lisboa LL, Hahn SR, et al. Simultaneous determination of vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices using liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2019;165:315-324.
Andriguetti, N. B., Lisboa, L. L., Hahn, S. R., Pagnussat, L. R., Antunes, M. V., & Linden, R. (2019). Simultaneous determination of vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices using liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 165, pp. 315-324. doi:10.1016/j.jpba.2018.12.023.
Andriguetti NB, et al. Simultaneous Determination of Vancomycin and Creatinine in Plasma Applied to Volumetric Absorptive Microsampling Devices Using Liquid Chromatography-tandem Mass Spectrometry. J Pharm Biomed Anal. 2019 Feb 20;165:315-324. PubMed PMID: 30579232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices using liquid chromatography-tandem mass spectrometry. AU - Andriguetti,Nadine B, AU - Lisboa,Letícia L, AU - Hahn,Siomara R, AU - Pagnussat,Lidiane R, AU - Antunes,Marina V, AU - Linden,Rafael, Y1 - 2018/12/14/ PY - 2018/09/26/received PY - 2018/12/12/revised PY - 2018/12/14/accepted PY - 2018/12/24/pubmed PY - 2019/4/4/medline PY - 2018/12/23/entrez KW - Creatinine KW - Therapeutic drug monitoring KW - Vancomycin KW - Volumetric absorptive microsampling SP - 315 EP - 324 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 165 N2 - Vancomycin (VCM) is a glycopeptide antibiotic, widely used against methicillin-resistant Staphylococcus aureus infections, and its clearance is highly correlated to creatinine (CRE) clearance. Usually, VCM and CRE levels are measured in serum or plasma specimens obtained from venous blood, after phlebotomy. As the majority of hospitals in Developing Countries do not have on-site access to VCM measurements, the availability of data on patient's VCM levels would involve the transport of liquid samples to specialized laboratories. An alternative to overcome the logistic difficulties of liquid biological specimens is the use of dried microsamples, such as plasma applied to volumetric absorptive microsampling (VAMS) devices. The aim of this study was to develop and validate a LC-MS/MS assay for the simultaneous determination of VCM and CRE in plasma applied to VAMS devices. VCM and CRE levels were determined in clinical relevant ranges with acceptable precision and accuracy and both compounds were stable in VAMS for up to two weeks at 45 °C. Concentrations measured in VAMS differed from those measured in plasma and the use of estimation approaches was necessary to estimate plasma concentrations. VCM levels in plasma can be estimated by multiplying VAMS measurements by the correction factor of 0.934. The unbiased estimation of CRE plasma levels from VAMS required the use of the equation CREplasma = 0.9808 * CREVAMS + 1.6621. VAMS can be used as an alternative for short-term storage and transportation of plasma at ambient and high temperatures, allowing a more widespread access to VCM therapeutic drug monitoring in limited-resources settings. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/30579232/Simultaneous_determination_of_vancomycin_and_creatinine_in_plasma_applied_to_volumetric_absorptive_microsampling_devices_using_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(18)32201-5 DB - PRIME DP - Unbound Medicine ER -