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Bergapten suppresses RANKL-induced osteoclastogenesis and ovariectomy-induced osteoporosis via suppression of NF-κB and JNK signaling pathways.
Biochem Biophys Res Commun. 2019 02 05; 509(2):329-334.BB

Abstract

Bergapten (BP), derived from Cnidium monnieri (L.) Cusson, is an ingredient widely used in traditional Chinese medicine and has important biological and pharmacological activities. However, the effect of BP on ovariectomy-induced osteoporosis and the underlying mechanism are not entirely clear. In this study, we investigated the effects of BP on ovariectomy-induced osteoporosis and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in vivo and in vitro, and explored the underlying mechanism. We found that BP treatment exerted beneficial effects on ovariectomy-induced osteoporosis in vivo. Further, BP attenuated osteoclastogenesis in bone marrow macrophages (BMMs) and RAW264.7 cells without any cytotoxicity. Additionally, BP specifically inhibited RANKL-induced NF-κB and JNK signaling,but did not suppress p38 and ERK. At the mRNA level, BP inhibits the OC-associated transcription factor NFATc1 and c-fos, thereby affecting the expression of OC differentiation-related genes. Moreover, BP disrupted the formation of F-actin rings, which are important for bone-resorbing activity, and impairs OC bone resorption. Therefore, BP may be a useful alternative therapy for post-menopausal osteoporosis.

Authors+Show Affiliations

Department of Orthopedics, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China; Multidisciplinary Therapy Center of Musculoskeletal Tumor, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. Electronic address: 1461698247@qq.com.Department of Orthopedics, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China; Multidisciplinary Therapy Center of Musculoskeletal Tumor, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. Electronic address: 774735825@qq.com.Department of Orthopedics, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China; Multidisciplinary Therapy Center of Musculoskeletal Tumor, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. Electronic address: daimin@medmail.com.cn.Department of Orthopedics, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China; Multidisciplinary Therapy Center of Musculoskeletal Tumor, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. Electronic address: C18270712885@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30579598

Citation

Chen, Guiping, et al. "Bergapten Suppresses RANKL-induced Osteoclastogenesis and Ovariectomy-induced Osteoporosis Via Suppression of NF-κB and JNK Signaling Pathways." Biochemical and Biophysical Research Communications, vol. 509, no. 2, 2019, pp. 329-334.
Chen G, Xu Q, Dai M, et al. Bergapten suppresses RANKL-induced osteoclastogenesis and ovariectomy-induced osteoporosis via suppression of NF-κB and JNK signaling pathways. Biochem Biophys Res Commun. 2019;509(2):329-334.
Chen, G., Xu, Q., Dai, M., & Liu, X. (2019). Bergapten suppresses RANKL-induced osteoclastogenesis and ovariectomy-induced osteoporosis via suppression of NF-κB and JNK signaling pathways. Biochemical and Biophysical Research Communications, 509(2), 329-334. https://doi.org/10.1016/j.bbrc.2018.12.112
Chen G, et al. Bergapten Suppresses RANKL-induced Osteoclastogenesis and Ovariectomy-induced Osteoporosis Via Suppression of NF-κB and JNK Signaling Pathways. Biochem Biophys Res Commun. 2019 02 5;509(2):329-334. PubMed PMID: 30579598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bergapten suppresses RANKL-induced osteoclastogenesis and ovariectomy-induced osteoporosis via suppression of NF-κB and JNK signaling pathways. AU - Chen,Guiping, AU - Xu,Qiang, AU - Dai,Min, AU - Liu,Xuqiang, Y1 - 2018/12/20/ PY - 2018/12/05/received PY - 2018/12/15/accepted PY - 2018/12/24/pubmed PY - 2019/10/23/medline PY - 2018/12/24/entrez KW - Bergapten KW - JNK KW - NF-κB KW - Osteoclast KW - Ovariectomy-induced osteoporosis SP - 329 EP - 334 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 509 IS - 2 N2 - Bergapten (BP), derived from Cnidium monnieri (L.) Cusson, is an ingredient widely used in traditional Chinese medicine and has important biological and pharmacological activities. However, the effect of BP on ovariectomy-induced osteoporosis and the underlying mechanism are not entirely clear. In this study, we investigated the effects of BP on ovariectomy-induced osteoporosis and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in vivo and in vitro, and explored the underlying mechanism. We found that BP treatment exerted beneficial effects on ovariectomy-induced osteoporosis in vivo. Further, BP attenuated osteoclastogenesis in bone marrow macrophages (BMMs) and RAW264.7 cells without any cytotoxicity. Additionally, BP specifically inhibited RANKL-induced NF-κB and JNK signaling,but did not suppress p38 and ERK. At the mRNA level, BP inhibits the OC-associated transcription factor NFATc1 and c-fos, thereby affecting the expression of OC differentiation-related genes. Moreover, BP disrupted the formation of F-actin rings, which are important for bone-resorbing activity, and impairs OC bone resorption. Therefore, BP may be a useful alternative therapy for post-menopausal osteoporosis. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/30579598/Bergapten_suppresses_RANKL_induced_osteoclastogenesis_and_ovariectomy_induced_osteoporosis_via_suppression_of_NF_κB_and_JNK_signaling_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(18)32761-X DB - PRIME DP - Unbound Medicine ER -