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Comparison of maximal bronchoconstriction in vivo and airway smooth muscle responses in vitro in nonasthmatic humans.
Am Rev Respir Dis. 1988 Aug; 138(2):321-6.AR

Abstract

We tested the hypothesis that maximal bronchoconstriction in humans in vivo is limited by the maximal contractility of airway smooth muscle by comparison of complete in vivo and in vitro dose-response curves to methacholine in 10 nonasthmatic subjects who were scheduled for thoracotomy because of malignancies. The provocative dose of methacholine that produced a 10 and 20% decrease of baseline FEV1 (PD10,20 FEV1) and the maximal fall in FEV1 (MFEV1) at the response plateau to inhaled methacholine were determined prior to surgery. Small airway smooth muscle preparations, obtained from the 10 resected lung tissue specimens, were examined in vitro to determine the sensitivity (-log EC50) and maximal isotonic shortening (Smax) to methacholine. In addition, the relaxation responses to the beta-agonist I-isoproterenol were measured. The degree of small airways disease (SAD) was examined histologically. Nine subjects showed a maximal response plateau to inhaled methacholine in vivo. The maximal fall in FEV1 at the plateau was 26 +/- 3%. All airway smooth muscle preparations (n = 30) contracted to methacholine (-log EC50, 5.94 +/- 0.09; Smax, 1320 +/- 219 micron) and relaxed to I-isoproterenol (-log EC50, 7.60 +/- 0.11; maximal relaxation [Rmax], 87 +/- 3%). No significant correlations were found between Smax or Rmax of the airway smooth muscle in vitro and the MFEV1 in vivo, and between -log EC50 for methacholine or I-isoproterenol in vitro and PD10 or PD20 FEV1 for methacholine in vivo. The severity of SAD was significantly correlated with the degree of baseline airflow limitation (p less than 0.05), but not with in vivo or in vitro responses to methacholine.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Paediatric Respiratory Diseases, University Hospital/Sophia Children's Hospital, Rotterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3057963

Citation

de Jongste, J C., et al. "Comparison of Maximal Bronchoconstriction in Vivo and Airway Smooth Muscle Responses in Vitro in Nonasthmatic Humans." The American Review of Respiratory Disease, vol. 138, no. 2, 1988, pp. 321-6.
de Jongste JC, Sterk PJ, Willems LN, et al. Comparison of maximal bronchoconstriction in vivo and airway smooth muscle responses in vitro in nonasthmatic humans. Am Rev Respir Dis. 1988;138(2):321-6.
de Jongste, J. C., Sterk, P. J., Willems, L. N., Mons, H., Timmers, M. C., & Kerrebijn, K. F. (1988). Comparison of maximal bronchoconstriction in vivo and airway smooth muscle responses in vitro in nonasthmatic humans. The American Review of Respiratory Disease, 138(2), 321-6.
de Jongste JC, et al. Comparison of Maximal Bronchoconstriction in Vivo and Airway Smooth Muscle Responses in Vitro in Nonasthmatic Humans. Am Rev Respir Dis. 1988;138(2):321-6. PubMed PMID: 3057963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of maximal bronchoconstriction in vivo and airway smooth muscle responses in vitro in nonasthmatic humans. AU - de Jongste,J C, AU - Sterk,P J, AU - Willems,L N, AU - Mons,H, AU - Timmers,M C, AU - Kerrebijn,K F, PY - 1988/8/1/pubmed PY - 1988/8/1/medline PY - 1988/8/1/entrez SP - 321 EP - 6 JF - The American review of respiratory disease JO - Am. Rev. Respir. Dis. VL - 138 IS - 2 N2 - We tested the hypothesis that maximal bronchoconstriction in humans in vivo is limited by the maximal contractility of airway smooth muscle by comparison of complete in vivo and in vitro dose-response curves to methacholine in 10 nonasthmatic subjects who were scheduled for thoracotomy because of malignancies. The provocative dose of methacholine that produced a 10 and 20% decrease of baseline FEV1 (PD10,20 FEV1) and the maximal fall in FEV1 (MFEV1) at the response plateau to inhaled methacholine were determined prior to surgery. Small airway smooth muscle preparations, obtained from the 10 resected lung tissue specimens, were examined in vitro to determine the sensitivity (-log EC50) and maximal isotonic shortening (Smax) to methacholine. In addition, the relaxation responses to the beta-agonist I-isoproterenol were measured. The degree of small airways disease (SAD) was examined histologically. Nine subjects showed a maximal response plateau to inhaled methacholine in vivo. The maximal fall in FEV1 at the plateau was 26 +/- 3%. All airway smooth muscle preparations (n = 30) contracted to methacholine (-log EC50, 5.94 +/- 0.09; Smax, 1320 +/- 219 micron) and relaxed to I-isoproterenol (-log EC50, 7.60 +/- 0.11; maximal relaxation [Rmax], 87 +/- 3%). No significant correlations were found between Smax or Rmax of the airway smooth muscle in vitro and the MFEV1 in vivo, and between -log EC50 for methacholine or I-isoproterenol in vitro and PD10 or PD20 FEV1 for methacholine in vivo. The severity of SAD was significantly correlated with the degree of baseline airflow limitation (p less than 0.05), but not with in vivo or in vitro responses to methacholine.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0003-0805 UR - https://www.unboundmedicine.com/medline/citation/3057963/Comparison_of_maximal_bronchoconstriction_in_vivo_and_airway_smooth_muscle_responses_in_vitro_in_nonasthmatic_humans_ L2 - http://www.atsjournals.org/doi/full/10.1164/ajrccm/138.2.321?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -