Tags

Type your tag names separated by a space and hit enter

Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage.
Oxid Med Cell Longev. 2018; 2018:1610751.OM

Abstract

The protection of retinal pigment epithelium (RPE) injury plays an important role in the prevention of or in delaying the pathological progress of retinal degeneration diseases, like age-related macular degeneration (AMD), diabetic retinopathy, and retinitis pigmentosa. Oxidative stress has been identified as a major inducer of RPE injury, which eventually could lead to a loss of vision. Kaempferol is a natural flavonoid widely distributed in many edible plants, fruits, and traditional medicines and has been reported to have antioxidant, anti-inflammatory, anticancer, and antimicrobial activities. The present study demonstrates that the total antioxidant capacity of kaempferol is approximately two times stronger than that of lutein which is also a natural antioxidant that is widely used in the prevention or treatment of AMD. Our data indicates that kaempferol protects human RPE cells (ARPE-19) from hydrogen peroxide- (H2O2-) induced oxidative cell damage and apoptosis through the signaling pathways involving Bax/Bcl-2 and caspase-3 molecules proofed by real-time PCR and Western blot results. Kaempferol also inhibits the upregulated vascular endothelial growth factor (VEGF) mRNA expression levels induced by H2O2 in ARPE-19 cells and affects the oxidation and antioxidant imbalanced system in ARPE-19 cells treated by H2O2 through the regulations of both the activities of reactive oxygen species (ROS) and superoxide dismutase (SOD). Furthermore, our in vivo experimental results show that in sodium iodate-induced retinal degeneration rat model, kaempferol could protect sodium iodate-induced pathological changes of retina tissue and retinal cells apoptosis as well as the upregulated VEGF protein expression in RPE cells. In summary, these novel findings demonstrate that kaempferol could protect oxidative stressed-human RPE cell damage through its antioxidant activity and antiapoptosis function, suggesting that kaempferol has a potential role in the prevention and therapeutic treatment of AMD or other retinal diseases mediated by oxidative stress.

Authors+Show Affiliations

The School of Pharmacy, He University, Shenyang, Liaoning 110163, China.The School of Pharmacy, He University, Shenyang, Liaoning 110163, China.The School of Pharmacy, He University, Shenyang, Liaoning 110163, China.The School of Pharmacy, He University, Shenyang, Liaoning 110163, China.The School of Pharmacy, He University, Shenyang, Liaoning 110163, China. Shenyang Industrial Technology Institute of Ophthalmology, Shenyang 110163, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30584457

Citation

Du, Weiwei, et al. "Protection of Kaempferol On Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage." Oxidative Medicine and Cellular Longevity, vol. 2018, 2018, p. 1610751.
Du W, An Y, He X, et al. Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage. Oxid Med Cell Longev. 2018;2018:1610751.
Du, W., An, Y., He, X., Zhang, D., & He, W. (2018). Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage. Oxidative Medicine and Cellular Longevity, 2018, 1610751. https://doi.org/10.1155/2018/1610751
Du W, et al. Protection of Kaempferol On Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage. Oxid Med Cell Longev. 2018;2018:1610751. PubMed PMID: 30584457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage. AU - Du,Weiwei, AU - An,Yuanlong, AU - He,Xiangdong, AU - Zhang,Donglei, AU - He,Wei, Y1 - 2018/11/21/ PY - 2018/08/07/received PY - 2018/09/27/revised PY - 2018/09/30/accepted PY - 2018/12/26/entrez PY - 2018/12/26/pubmed PY - 2019/1/23/medline SP - 1610751 EP - 1610751 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2018 N2 - The protection of retinal pigment epithelium (RPE) injury plays an important role in the prevention of or in delaying the pathological progress of retinal degeneration diseases, like age-related macular degeneration (AMD), diabetic retinopathy, and retinitis pigmentosa. Oxidative stress has been identified as a major inducer of RPE injury, which eventually could lead to a loss of vision. Kaempferol is a natural flavonoid widely distributed in many edible plants, fruits, and traditional medicines and has been reported to have antioxidant, anti-inflammatory, anticancer, and antimicrobial activities. The present study demonstrates that the total antioxidant capacity of kaempferol is approximately two times stronger than that of lutein which is also a natural antioxidant that is widely used in the prevention or treatment of AMD. Our data indicates that kaempferol protects human RPE cells (ARPE-19) from hydrogen peroxide- (H2O2-) induced oxidative cell damage and apoptosis through the signaling pathways involving Bax/Bcl-2 and caspase-3 molecules proofed by real-time PCR and Western blot results. Kaempferol also inhibits the upregulated vascular endothelial growth factor (VEGF) mRNA expression levels induced by H2O2 in ARPE-19 cells and affects the oxidation and antioxidant imbalanced system in ARPE-19 cells treated by H2O2 through the regulations of both the activities of reactive oxygen species (ROS) and superoxide dismutase (SOD). Furthermore, our in vivo experimental results show that in sodium iodate-induced retinal degeneration rat model, kaempferol could protect sodium iodate-induced pathological changes of retina tissue and retinal cells apoptosis as well as the upregulated VEGF protein expression in RPE cells. In summary, these novel findings demonstrate that kaempferol could protect oxidative stressed-human RPE cell damage through its antioxidant activity and antiapoptosis function, suggesting that kaempferol has a potential role in the prevention and therapeutic treatment of AMD or other retinal diseases mediated by oxidative stress. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/30584457/Protection_of_Kaempferol_on_Oxidative_Stress_Induced_Retinal_Pigment_Epithelial_Cell_Damage_ L2 - https://dx.doi.org/10.1155/2018/1610751 DB - PRIME DP - Unbound Medicine ER -