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[Effect of Acupuncture Intervention on Depression Behavior and Expression of ERK 1/2 and BDNF in Prefrontal Cortex in Chronic Unpredictable Mild Stress Induced Depression Rats].
Zhen Ci Yan Jiu. 2018 Nov 25; 43(11):705-10.ZC

Abstract

OBJECTIVE

To investigate the effect of acupuncture intervention on the depression behavior and expression of extracellular signal-regulated protein kinases (ERK 1/2), p-ERK 1/2 and brain-derived neurotrophic factor (BDNF) in the prefrontal cortex of chronic unpredictable mild stress (CUMS) induced depression rats, so as to explore its antidepressant mechanism.

METHODS

Sixty-four male SD rats were randomly divided into control, model, acupuncture, Fluoxetine, model + Dimethyl sulfoxide (DMSO), model + PD 98059(an ERK pathway inhibitor), acupuncture + PD 98059 and Fluoxetine + PD 98059 groups (n=8 rats in each). The CUMS depression model was established by using chronic mild and unpredictable stress methods for 21 days. Manual acupuncture stimulation was applied to "Baihui" (GV 20) and "Yintang" (GV 29) for 10 min before modeling, once daily for 21 days. Fluoxetine hydrochloride suspension (1.8 mg•kg-1•d-1) was given to rats of the Fluoxetine group and Fluo-xetine + PD 98059 group by gavage 30 min before CUMS. PD 98059 (dissolved in DMSO, 10 µL) was administered to rats of model + PD 98059 group, acupuncture + PD 98059 and Fluoxetine + PD 98059 group, and DMSO (10 µL) to rats of model + DMSO group by intracerebroventricular injection 1 h before CUMS. Sucrose consumption test was carried out to evaluate the depressive behavior. Western blot was performed to detect the expression of ERK 1/2, p-ERK 1/2 and BDNF of prefrontal cortex.

RESULTS

Compared with the control group, the sucrose consumption and the expression levels of p-ERK 1/2 and BDNF protein in the prefrontal cortex were significantly reduced in the model and model+DMSO group (P<0. 01). After the intervention, modeling induced decrease of the sucrose consumption, and p-ERK 1/2 and BDNF expression was significantly up-regulated in both acupuncture and Fluoxetine groups (P<0.01, P<0.05), but not in the model+PD 98059, Fluoxetine +PD 98059 and acupuncture+PD 98059 groups (P>0.05). No significant differences were found among the model+PD 98059, Fluoxetine +PD 98059 and acupuncture+PD 98059 groups in the sucrose consumption, and ERK 1/2, p-ERK 1/2 and BDNF expression levels (P>0.05), and in the expression levels of ERK 1/2 protein among the 8 groups (P>0.05).

CONCLUSION

Acupuncture intervention has an anti-depressive role in CUMS induced depression rats, which may be related to its effects in up-regulating the expression of p-ERK 1/2 and BDNF in the prefrontal cortex tissue.

Authors+Show Affiliations

School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.Chengdu University of Chinese Medicine, Chengdu 610075.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Research Center of Mental and Neurological Disorders, School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30585467

Citation

Li, Xiang, et al. "[Effect of Acupuncture Intervention On Depression Behavior and Expression of ERK 1/2 and BDNF in Prefrontal Cortex in Chronic Unpredictable Mild Stress Induced Depression Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 11, 2018, pp. 705-10.
Li X, Xu MM, Jiang HL, et al. [Effect of Acupuncture Intervention on Depression Behavior and Expression of ERK 1/2 and BDNF in Prefrontal Cortex in Chronic Unpredictable Mild Stress Induced Depression Rats]. Zhen Ci Yan Jiu. 2018;43(11):705-10.
Li, X., Xu, M. M., Jiang, H. L., Lu, J., Zhang, X. H., Zhao, B. C., Yang, X. J., Sun, Y., Gao, X. Z., Liang, X. C., Yu, Q. Y., Feng, S. X., Li, Y. F., Yu, M., & Tu, Y. (2018). [Effect of Acupuncture Intervention on Depression Behavior and Expression of ERK 1/2 and BDNF in Prefrontal Cortex in Chronic Unpredictable Mild Stress Induced Depression Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(11), 705-10. https://doi.org/10.13702/j.1000-0607.180288
Li X, et al. [Effect of Acupuncture Intervention On Depression Behavior and Expression of ERK 1/2 and BDNF in Prefrontal Cortex in Chronic Unpredictable Mild Stress Induced Depression Rats]. Zhen Ci Yan Jiu. 2018 Nov 25;43(11):705-10. PubMed PMID: 30585467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Acupuncture Intervention on Depression Behavior and Expression of ERK 1/2 and BDNF in Prefrontal Cortex in Chronic Unpredictable Mild Stress Induced Depression Rats]. AU - Li,Xiang, AU - Xu,Ming-Min, AU - Jiang,Hui-Li, AU - Lu,Jun, AU - Zhang,Xu-Hui, AU - Zhao,Bing-Cong, AU - Yang,Xin-Jing, AU - Sun,Yang, AU - Gao,Xing-Zhou, AU - Liang,Xing-Chen, AU - Yu,Qiu-Yun, AU - Feng,Shi-Xing, AU - Li,Yu-Fei, AU - Yu,Miao, AU - Tu,Ya, PY - 2018/12/27/entrez PY - 2018/12/27/pubmed PY - 2019/4/9/medline KW - Acupuncture KW - Brain-derived neurotrophic factor KW - Chronic unpredictable mild stress KW - Depression KW - Extracellular regulated protein kinases KW - PD 98059 SP - 705 EP - 10 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 11 N2 - OBJECTIVE: To investigate the effect of acupuncture intervention on the depression behavior and expression of extracellular signal-regulated protein kinases (ERK 1/2), p-ERK 1/2 and brain-derived neurotrophic factor (BDNF) in the prefrontal cortex of chronic unpredictable mild stress (CUMS) induced depression rats, so as to explore its antidepressant mechanism. METHODS: Sixty-four male SD rats were randomly divided into control, model, acupuncture, Fluoxetine, model + Dimethyl sulfoxide (DMSO), model + PD 98059(an ERK pathway inhibitor), acupuncture + PD 98059 and Fluoxetine + PD 98059 groups (n=8 rats in each). The CUMS depression model was established by using chronic mild and unpredictable stress methods for 21 days. Manual acupuncture stimulation was applied to "Baihui" (GV 20) and "Yintang" (GV 29) for 10 min before modeling, once daily for 21 days. Fluoxetine hydrochloride suspension (1.8 mg•kg-1•d-1) was given to rats of the Fluoxetine group and Fluo-xetine + PD 98059 group by gavage 30 min before CUMS. PD 98059 (dissolved in DMSO, 10 µL) was administered to rats of model + PD 98059 group, acupuncture + PD 98059 and Fluoxetine + PD 98059 group, and DMSO (10 µL) to rats of model + DMSO group by intracerebroventricular injection 1 h before CUMS. Sucrose consumption test was carried out to evaluate the depressive behavior. Western blot was performed to detect the expression of ERK 1/2, p-ERK 1/2 and BDNF of prefrontal cortex. RESULTS: Compared with the control group, the sucrose consumption and the expression levels of p-ERK 1/2 and BDNF protein in the prefrontal cortex were significantly reduced in the model and model+DMSO group (P<0. 01). After the intervention, modeling induced decrease of the sucrose consumption, and p-ERK 1/2 and BDNF expression was significantly up-regulated in both acupuncture and Fluoxetine groups (P<0.01, P<0.05), but not in the model+PD 98059, Fluoxetine +PD 98059 and acupuncture+PD 98059 groups (P>0.05). No significant differences were found among the model+PD 98059, Fluoxetine +PD 98059 and acupuncture+PD 98059 groups in the sucrose consumption, and ERK 1/2, p-ERK 1/2 and BDNF expression levels (P>0.05), and in the expression levels of ERK 1/2 protein among the 8 groups (P>0.05). CONCLUSION: Acupuncture intervention has an anti-depressive role in CUMS induced depression rats, which may be related to its effects in up-regulating the expression of p-ERK 1/2 and BDNF in the prefrontal cortex tissue. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/30585467/[Effect_of_Acupuncture_Intervention_on_Depression_Behavior_and_Expression_of_ERK_1/2_and_BDNF_in_Prefrontal_Cortex_in_Chronic_Unpredictable_Mild_Stress_Induced_Depression_Rats]_ L2 - https://medlineplus.gov/depression.html DB - PRIME DP - Unbound Medicine ER -