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Integrated analysis of dysregulated long non-coding RNAs/microRNAs/mRNAs in metastasis of lung adenocarcinoma.
J Transl Med. 2018 12 27; 16(1):372.JT

Abstract

BACKGROUND

Lung adenocarcinoma (LUAD), largely remains a primary cause of cancer-related death worldwide. The molecular mechanisms in LUAD metastasis have not been completely uncovered.

METHODS

In this study, we identified differentially expressed genes (DEGs), miRNAs (DEMs) and lncRNAs (DELs) underlying metastasis of LUAD from The Cancer Genome Atlas database. Intersection mRNAs were used to perform gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and co-expression network analysis. In addition, survival analyses of intersection mRNAs were conducted. Finally, intersection mRNAs, miRNAs and lncRNAs were subjected to construct miRNA-mRNA-lncRNA network.

RESULTS

A total of 1015 DEGs, 54 DEMs and 22 DELs were identified in LUAD metastasis and non-metastasis samples. GO and KEGG pathway analysis had proven that the functions of intersection mRNAs were closely related with many important processes in cancer pathogenesis. Among the co-expression interactions network, 22 genes in the co-expression network were over the degree 20. These genes imply that they have connections with many other gene nodes. In addition, 14 target genes (ARHGAP11A, ASPM, HELLS, PRC1, TMPO, ARHGAP30, CD52, IL16, IRF8, P2RY13, PRKCB, PTPRC, SASH3 and TRAF3IP3) were found to be associated with survival in patients with LUAD significantly (log-rank P < 0.05). Two lncRNAs (LOC96610 and ADAM6) acting as ceRNAs were identified based on the miRNA-mRNA-lncRNA network.

CONCLUSIONS

Taken together, the results may provide a novel perspective to develop a multiple gene diagnostic tool for LUAD prognosis, which might also provide potential biomarkers or therapeutic targets for LUAD.

Authors+Show Affiliations

Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China. Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China. National Engineering Laboratory for Internet Medical Systems and Applications, Zhengzhou, 450052, Henan, China.Department of Clinical Laboratory, The Third People's Hospital of Henan Province, Zhengzhou, 450052, Henan, China.Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.National Engineering Laboratory for Internet Medical Systems and Applications, Zhengzhou, 450052, Henan, China.Department of Clinical Laboratory, The Third People's Hospital of Henan Province, Zhengzhou, 450052, Henan, China.Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Key Laboratory of Laboratory Medicine of Henan Province, Zhengzhou, 450052, Henan, China. qindongchun@163.com.Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China. jiezhaoz2016@163.com. Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China. jiezhaoz2016@163.com. National Engineering Laboratory for Internet Medical Systems and Applications, Zhengzhou, 450052, Henan, China. jiezhaoz2016@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30587197

Citation

Li, Lifeng, et al. "Integrated Analysis of Dysregulated Long Non-coding RNAs/microRNAs/mRNAs in Metastasis of Lung Adenocarcinoma." Journal of Translational Medicine, vol. 16, no. 1, 2018, p. 372.
Li L, Peng M, Xue W, et al. Integrated analysis of dysregulated long non-coding RNAs/microRNAs/mRNAs in metastasis of lung adenocarcinoma. J Transl Med. 2018;16(1):372.
Li, L., Peng, M., Xue, W., Fan, Z., Wang, T., Lian, J., Zhai, Y., Lian, W., Qin, D., & Zhao, J. (2018). Integrated analysis of dysregulated long non-coding RNAs/microRNAs/mRNAs in metastasis of lung adenocarcinoma. Journal of Translational Medicine, 16(1), 372. https://doi.org/10.1186/s12967-018-1732-z
Li L, et al. Integrated Analysis of Dysregulated Long Non-coding RNAs/microRNAs/mRNAs in Metastasis of Lung Adenocarcinoma. J Transl Med. 2018 12 27;16(1):372. PubMed PMID: 30587197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrated analysis of dysregulated long non-coding RNAs/microRNAs/mRNAs in metastasis of lung adenocarcinoma. AU - Li,Lifeng, AU - Peng,Mengle, AU - Xue,Wenhua, AU - Fan,Zhirui, AU - Wang,Tian, AU - Lian,Jingyao, AU - Zhai,Yunkai, AU - Lian,Wenping, AU - Qin,Dongchun, AU - Zhao,Jie, Y1 - 2018/12/27/ PY - 2018/07/17/received PY - 2018/12/06/accepted PY - 2018/12/28/entrez PY - 2018/12/28/pubmed PY - 2019/6/19/medline KW - Biomarker KW - Lung adenocarcinoma KW - Metastasis KW - lncRNAs SP - 372 EP - 372 JF - Journal of translational medicine JO - J Transl Med VL - 16 IS - 1 N2 - BACKGROUND: Lung adenocarcinoma (LUAD), largely remains a primary cause of cancer-related death worldwide. The molecular mechanisms in LUAD metastasis have not been completely uncovered. METHODS: In this study, we identified differentially expressed genes (DEGs), miRNAs (DEMs) and lncRNAs (DELs) underlying metastasis of LUAD from The Cancer Genome Atlas database. Intersection mRNAs were used to perform gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and co-expression network analysis. In addition, survival analyses of intersection mRNAs were conducted. Finally, intersection mRNAs, miRNAs and lncRNAs were subjected to construct miRNA-mRNA-lncRNA network. RESULTS: A total of 1015 DEGs, 54 DEMs and 22 DELs were identified in LUAD metastasis and non-metastasis samples. GO and KEGG pathway analysis had proven that the functions of intersection mRNAs were closely related with many important processes in cancer pathogenesis. Among the co-expression interactions network, 22 genes in the co-expression network were over the degree 20. These genes imply that they have connections with many other gene nodes. In addition, 14 target genes (ARHGAP11A, ASPM, HELLS, PRC1, TMPO, ARHGAP30, CD52, IL16, IRF8, P2RY13, PRKCB, PTPRC, SASH3 and TRAF3IP3) were found to be associated with survival in patients with LUAD significantly (log-rank P < 0.05). Two lncRNAs (LOC96610 and ADAM6) acting as ceRNAs were identified based on the miRNA-mRNA-lncRNA network. CONCLUSIONS: Taken together, the results may provide a novel perspective to develop a multiple gene diagnostic tool for LUAD prognosis, which might also provide potential biomarkers or therapeutic targets for LUAD. SN - 1479-5876 UR - https://www.unboundmedicine.com/medline/citation/30587197/Integrated_analysis_of_dysregulated_long_non_coding_RNAs/microRNAs/mRNAs_in_metastasis_of_lung_adenocarcinoma_ L2 - https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-018-1732-z DB - PRIME DP - Unbound Medicine ER -