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Synergetic effect of vancomycin loaded silver nanoparticles for enhanced antibacterial activity.
Colloids Surf B Biointerfaces. 2019 Apr 01; 176:62-69.CS

Abstract

In recent years, there is significant growth in the bacterial resistance to various classic antibiotics. This has opened and enhanced the field of metal nanoparticles and antibiotic-metal nanoparticle complex. This research was designed to load a glycopeptide antibiotic named vancomycin on citrate-capped silver nanoparticles to enhance its antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Colloidal solution of silver nanoparticles (AgNPs) was prepared by chemical reduction method using silver nitrate (AgNO3) as a precursor in the presence of ionic surfactant trisodium-citrate which acts as a both capping and reducing agent. Synthesized nanoparticles were functionalized with vancomycin to form nano-drug complex (Van@AgNPs). Various analytical techniques such as UV-vis absorption spectra, FTIR, DLS, TEM and XRD were carried out to study the loading and interaction of drug with silver nanoparticles. The observed shift in SPR peak of UV-vis and various reflections of XRD spectra is attributed towards the loading of vancomycin on silver nanoparticle surface. FTIR studies shows the hydrogen bonding between vancomycin and silver nanoparticles through NH (amine) group of vancomycin and oxygen of anionic citrate. The increase in average particle size and particle size distribution of vancomycin-loaded nanoparticles in comparison to bare NPs also hints the drug loading. Agar well diffusion method was used to study the synergetic antibacterial activity of vancomycin-loaded silver nanoparticles against both test strains. The well diffusion test showed the notable enhancement in antibacterial activity against both class of bacteria. This enhancement has been observed to be synergetic rather than the additive.

Authors+Show Affiliations

Department of Physics, Panjab University, Chandigarh, 160014, India.Department of Biophysics, Panjab University, Chandigarh, 160014, India.Centre for Medical Physics, Panjab University, Chandigarh, 160014, India.Department of Environment Studies, Panjab University, Chandigarh, 160014, India.Department of Physics, Panjab University, Chandigarh, 160014, India. Electronic address: rajeshbaboria@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30594704

Citation

Kaur, Amritpal, et al. "Synergetic Effect of Vancomycin Loaded Silver Nanoparticles for Enhanced Antibacterial Activity." Colloids and Surfaces. B, Biointerfaces, vol. 176, 2019, pp. 62-69.
Kaur A, Preet S, Kumar V, et al. Synergetic effect of vancomycin loaded silver nanoparticles for enhanced antibacterial activity. Colloids Surf B Biointerfaces. 2019;176:62-69.
Kaur, A., Preet, S., Kumar, V., Kumar, R., & Kumar, R. (2019). Synergetic effect of vancomycin loaded silver nanoparticles for enhanced antibacterial activity. Colloids and Surfaces. B, Biointerfaces, 176, 62-69. https://doi.org/10.1016/j.colsurfb.2018.12.043
Kaur A, et al. Synergetic Effect of Vancomycin Loaded Silver Nanoparticles for Enhanced Antibacterial Activity. Colloids Surf B Biointerfaces. 2019 Apr 1;176:62-69. PubMed PMID: 30594704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergetic effect of vancomycin loaded silver nanoparticles for enhanced antibacterial activity. AU - Kaur,Amritpal, AU - Preet,Simran, AU - Kumar,Vivek, AU - Kumar,Rajeev, AU - Kumar,Rajesh, Y1 - 2018/12/18/ PY - 2018/10/08/received PY - 2018/12/15/revised PY - 2018/12/17/accepted PY - 2018/12/31/pubmed PY - 2019/7/12/medline PY - 2018/12/31/entrez KW - Antibacterial efficacy KW - Drug coated AgNPs KW - Escherichia coli KW - Staphylococcus aureus KW - Synergetic effect SP - 62 EP - 69 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 176 N2 - In recent years, there is significant growth in the bacterial resistance to various classic antibiotics. This has opened and enhanced the field of metal nanoparticles and antibiotic-metal nanoparticle complex. This research was designed to load a glycopeptide antibiotic named vancomycin on citrate-capped silver nanoparticles to enhance its antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Colloidal solution of silver nanoparticles (AgNPs) was prepared by chemical reduction method using silver nitrate (AgNO3) as a precursor in the presence of ionic surfactant trisodium-citrate which acts as a both capping and reducing agent. Synthesized nanoparticles were functionalized with vancomycin to form nano-drug complex (Van@AgNPs). Various analytical techniques such as UV-vis absorption spectra, FTIR, DLS, TEM and XRD were carried out to study the loading and interaction of drug with silver nanoparticles. The observed shift in SPR peak of UV-vis and various reflections of XRD spectra is attributed towards the loading of vancomycin on silver nanoparticle surface. FTIR studies shows the hydrogen bonding between vancomycin and silver nanoparticles through NH (amine) group of vancomycin and oxygen of anionic citrate. The increase in average particle size and particle size distribution of vancomycin-loaded nanoparticles in comparison to bare NPs also hints the drug loading. Agar well diffusion method was used to study the synergetic antibacterial activity of vancomycin-loaded silver nanoparticles against both test strains. The well diffusion test showed the notable enhancement in antibacterial activity against both class of bacteria. This enhancement has been observed to be synergetic rather than the additive. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/30594704/Synergetic_effect_of_vancomycin_loaded_silver_nanoparticles_for_enhanced_antibacterial_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(18)30924-X DB - PRIME DP - Unbound Medicine ER -