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A non-conserved sequence in the 5'region of the CYH2 intron from Saccharomyces cerevisiae controls splicing efficiency of the pre-mRNA.
Yeast. 1988 Sep; 4(3):209-17.Y

Abstract

The CYH2 gene from Saccharomyces cerevisiae containing one 510 bp intron is spliced inefficiently. We have shown previously that a non-conserved sequence within the intron is responsible for this low splicing efficiency. Using synthetic oligonucleotides comprising the identified region we show in this report that a very short region contains the specificity to act negatively on the splicing efficiency of the CYH2 gene. Furthermore, this sequence influences the splicing efficiency only when it is placed close to the 5' splice site of the gene. Investigations with chimeric CYH2/beta-actin genes show that this sequence acts independent from its natural surroundings. We propose that this sequence might interact with splicing factor(s).

Authors+Show Affiliations

Swida U
Institut für Biochemie und Molekularbiologie, Freie Universität Berlin.
Thüroff E
No affiliation info available
Steinert E
No affiliation info available
Käufer NF
No affiliation info available

MeSH

Base SequenceBlotting, NorthernGenes, FungalIntronsMolecular Sequence DataPlasmidsRNA PrecursorsRNA SplicingRNA, FungalRNA, MessengerSaccharomyces cerevisiaeTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3059718

Citation

Swida, U, et al. "A Non-conserved Sequence in the 5'region of the CYH2 Intron From Saccharomyces Cerevisiae Controls Splicing Efficiency of the Pre-mRNA." Yeast (Chichester, England), vol. 4, no. 3, 1988, pp. 209-17.
Swida U, Thüroff E, Steinert E, et al. A non-conserved sequence in the 5'region of the CYH2 intron from Saccharomyces cerevisiae controls splicing efficiency of the pre-mRNA. Yeast. 1988;4(3):209-17.
Swida, U., Thüroff, E., Steinert, E., & Käufer, N. F. (1988). A non-conserved sequence in the 5'region of the CYH2 intron from Saccharomyces cerevisiae controls splicing efficiency of the pre-mRNA. Yeast (Chichester, England), 4(3), 209-17.
Swida U, et al. A Non-conserved Sequence in the 5'region of the CYH2 Intron From Saccharomyces Cerevisiae Controls Splicing Efficiency of the Pre-mRNA. Yeast. 1988;4(3):209-17. PubMed PMID: 3059718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A non-conserved sequence in the 5'region of the CYH2 intron from Saccharomyces cerevisiae controls splicing efficiency of the pre-mRNA. AU - Swida,U, AU - Thüroff,E, AU - Steinert,E, AU - Käufer,N F, PY - 1988/9/1/pubmed PY - 1988/9/1/medline PY - 1988/9/1/entrez SP - 209 EP - 17 JF - Yeast (Chichester, England) JO - Yeast VL - 4 IS - 3 N2 - The CYH2 gene from Saccharomyces cerevisiae containing one 510 bp intron is spliced inefficiently. We have shown previously that a non-conserved sequence within the intron is responsible for this low splicing efficiency. Using synthetic oligonucleotides comprising the identified region we show in this report that a very short region contains the specificity to act negatively on the splicing efficiency of the CYH2 gene. Furthermore, this sequence influences the splicing efficiency only when it is placed close to the 5' splice site of the gene. Investigations with chimeric CYH2/beta-actin genes show that this sequence acts independent from its natural surroundings. We propose that this sequence might interact with splicing factor(s). SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/3059718/A_non_conserved_sequence_in_the_5'region_of_the_CYH2_intron_from_Saccharomyces_cerevisiae_controls_splicing_efficiency_of_the_pre_mRNA_ DB - PRIME DP - Unbound Medicine ER -