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Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology.
Molecules. 2018 Dec 29; 24(1)M

Abstract

The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from Sophora flavescens were investigated in this study. In order to optimize the extraction of flavonoids from Sophora flavescens, the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 °C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited Sophora flavescens flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction.

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Authors+Show Affiliations

Zhou J
College of Life Science, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. zhoujing00727@163.com.
Zhang L
Second Clinical Medical College, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. ZLC1998327@163.com.
Li Q
College of Life Science, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. 18758132027@163.com.
Jin W
College of Pharmacy, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. jin_weifeng@126.com.
Chen W
College of Basic medical, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. chengweiyan18@sina.com.
Han J
College of Basic medical, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. hanjin20181127@sina.com.
Zhang Y
College of Life Science, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou 310053, Zhejiang, China. yannoo7376@sina.com.

MeSH

Analysis of VarianceAntioxidantsChemical FractionationChromatography, High Pressure LiquidFlavonoidsMolecular StructurePlant ExtractsSophoraTemperatureTime FactorsUltrasonic Waves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30597974

Citation

Zhou, Jing, et al. "Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids From Sophora Flavescens Using Response Surface Methodology." Molecules (Basel, Switzerland), vol. 24, no. 1, 2018.
Zhou J, Zhang L, Li Q, et al. Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology. Molecules. 2018;24(1).
Zhou, J., Zhang, L., Li, Q., Jin, W., Chen, W., Han, J., & Zhang, Y. (2018). Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology. Molecules (Basel, Switzerland), 24(1). https://doi.org/10.3390/molecules24010112
Zhou J, et al. Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids From Sophora Flavescens Using Response Surface Methodology. Molecules. 2018 Dec 29;24(1) PubMed PMID: 30597974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology. AU - Zhou,Jing, AU - Zhang,Lincheng, AU - Li,Qinping, AU - Jin,Weifeng, AU - Chen,Weiyan, AU - Han,Jin, AU - Zhang,Yuyan, Y1 - 2018/12/29/ PY - 2018/11/21/received PY - 2018/12/23/revised PY - 2018/12/24/accepted PY - 2019/1/2/entrez PY - 2019/1/2/pubmed PY - 2019/2/23/medline KW - Sophora flavescens KW - antioxidant activity KW - flavonoids KW - response surface methodology KW - ultrasound-assisted extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 1 N2 - The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from Sophora flavescens were investigated in this study. In order to optimize the extraction of flavonoids from Sophora flavescens, the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 °C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited Sophora flavescens flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30597974/Simultaneous_Optimization_for_Ultrasound_Assisted_Extraction_and_Antioxidant_Activity_of_Flavonoids_from_Sophora_flavescens_Using_Response_Surface_Methodology_ DB - PRIME DP - Unbound Medicine ER -