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Photoelectrochemical DNA biosensor based on g-C3N4/MoS2 2D/2D heterojunction electrode matrix and co-sensitization amplification with CdSe QDs for the sensitive detection of ssDNA.
Anal Chim Acta. 2019 Feb 07; 1048:42-49.AC

Abstract

A novel enhanced photoelectrochemical (PEC) DNA biosensor, based on a compact heterojunction g-C3N4/MoS2 and co-sensitization effect with CdSe quantum dots (QDs), was first proposed for simple and accurate analysis of a short ssDNA. In this work, the g-C3N4/MoS2 was successfully synthesized and used as the electrode matrix material to construct PEC biosensor. 2D/2D heterojunction was formed between g-C3N4 and MoS2, which could promote the separation of photogenerated electron-hole pairs resulting in an enhanced photocurrent. In the presence of target DNA, CdSe QDs labeled reporter DNA was complementary pairing with target DNA which was specific recognized by capture DNA loading on self-assembled CdS QDs film, leading to close contact between CdSe QDs and g-C3N4/MoS2 modified electrode surface, thereby resulting in the enhanced photocurrent intensity due to the co-sensitization effect. Under the optimal operating conditions, the photoelectrochemical biosensor demonstrated favorable accuracy and could respond to 0.32 pM (S/N = 3) with a linear concentration range from 1.0 pM to 2.0 μM. Moreover, the proposed PEC DNA biosensor exhibits high sensitivity, excellent specificity, acceptable reproducibility and accuracy, showing a promising potential in DNA bioanalysis and other relative fields.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li PP
Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, PR China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China.
Liu XP
Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, PR China.
Mao CJ
Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, PR China. Electronic address: maochangjie@sina.com.
Jin BK
Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, PR China.
Zhu JJ
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China. Electronic address: jjzhu@nju.edu.cn.

MeSH

Biosensing TechniquesCadmium CompoundsDNA, Single-StrandedDisulfidesElectrochemical TechniquesElectrodesLightLimit of DetectionMolybdenumNitrilesNucleic Acid HybridizationPhotochemistryQuantum DotsSelenium Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30598156

Citation

Li, Pan-Pan, et al. "Photoelectrochemical DNA Biosensor Based On g-C3N4/MoS2 2D/2D Heterojunction Electrode Matrix and Co-sensitization Amplification With CdSe QDs for the Sensitive Detection of SsDNA." Analytica Chimica Acta, vol. 1048, 2019, pp. 42-49.
Li PP, Liu XP, Mao CJ, et al. Photoelectrochemical DNA biosensor based on g-C3N4/MoS2 2D/2D heterojunction electrode matrix and co-sensitization amplification with CdSe QDs for the sensitive detection of ssDNA. Anal Chim Acta. 2019;1048:42-49.
Li, P. P., Liu, X. P., Mao, C. J., Jin, B. K., & Zhu, J. J. (2019). Photoelectrochemical DNA biosensor based on g-C3N4/MoS2 2D/2D heterojunction electrode matrix and co-sensitization amplification with CdSe QDs for the sensitive detection of ssDNA. Analytica Chimica Acta, 1048, 42-49. https://doi.org/10.1016/j.aca.2018.09.063
Li PP, et al. Photoelectrochemical DNA Biosensor Based On g-C3N4/MoS2 2D/2D Heterojunction Electrode Matrix and Co-sensitization Amplification With CdSe QDs for the Sensitive Detection of SsDNA. Anal Chim Acta. 2019 Feb 7;1048:42-49. PubMed PMID: 30598156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photoelectrochemical DNA biosensor based on g-C3N4/MoS2 2D/2D heterojunction electrode matrix and co-sensitization amplification with CdSe QDs for the sensitive detection of ssDNA. AU - Li,Pan-Pan, AU - Liu,Xing-Pei, AU - Mao,Chang-Jie, AU - Jin,Bao-Kang, AU - Zhu,Jun-Jie, Y1 - 2018/10/01/ PY - 2018/09/09/received PY - 2018/09/20/revised PY - 2018/09/26/accepted PY - 2019/1/2/entrez PY - 2019/1/2/pubmed PY - 2019/3/15/medline KW - CdSe quantum dots KW - Co-sensitive KW - G-C(3)N(4) KW - Heterojunction KW - MoS(2) KW - Photoelectrochemical SP - 42 EP - 49 JF - Analytica chimica acta JO - Anal Chim Acta VL - 1048 N2 - A novel enhanced photoelectrochemical (PEC) DNA biosensor, based on a compact heterojunction g-C3N4/MoS2 and co-sensitization effect with CdSe quantum dots (QDs), was first proposed for simple and accurate analysis of a short ssDNA. In this work, the g-C3N4/MoS2 was successfully synthesized and used as the electrode matrix material to construct PEC biosensor. 2D/2D heterojunction was formed between g-C3N4 and MoS2, which could promote the separation of photogenerated electron-hole pairs resulting in an enhanced photocurrent. In the presence of target DNA, CdSe QDs labeled reporter DNA was complementary pairing with target DNA which was specific recognized by capture DNA loading on self-assembled CdS QDs film, leading to close contact between CdSe QDs and g-C3N4/MoS2 modified electrode surface, thereby resulting in the enhanced photocurrent intensity due to the co-sensitization effect. Under the optimal operating conditions, the photoelectrochemical biosensor demonstrated favorable accuracy and could respond to 0.32 pM (S/N = 3) with a linear concentration range from 1.0 pM to 2.0 μM. Moreover, the proposed PEC DNA biosensor exhibits high sensitivity, excellent specificity, acceptable reproducibility and accuracy, showing a promising potential in DNA bioanalysis and other relative fields. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/30598156/Photoelectrochemical_DNA_biosensor_based_on_g_C3N4/MoS2_2D/2D_heterojunction_electrode_matrix_and_co_sensitization_amplification_with_CdSe_QDs_for_the_sensitive_detection_of_ssDNA_ DB - PRIME DP - Unbound Medicine ER -