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Pharmacological and polyphenolic profiles of Phyllanthus phillyreifolius var. commersonii Müll. Arg: An unexplored endemic species from Mauritius.
Food Res Int. 2019 01; 115:425-438.FR

Abstract

Phyllanthus phillyreifolius var. commersonii Müll. Arg is an endemic plant of Mauritius. To date, no study has been performed concerning its polyphenolic profile and pharmacological properties. In this study, a decoction (water), ethyl acetate and methanol extracts of the aerial parts of P. phillyreifolius, obtained from different extraction procedures (maceration and Soxhlet), were studied for antibacterial, antioxidant, anticancer, and enzyme inhibitory properties along with their polyphenolic profile. The ethyl acetate macerated extract showed high antibacterial activity against B. cereus (MIC = 0.293 mg/mL) and E. coli (MIC = 0.417 mg/mL) while S. epidermidis was most susceptible to the ethyl acetate-Soxhlet extract (MIC = 0.521 mg/mL). The methanol-Soxhlet extract displayed the most potent cupric and ferric reducing power, and metal chelating effect, while the macerated methanolic extract was the most effective DPPH and ABTS scavenger, and BChE inhibitor. Only the ethyl acetate-Soxhlet extract exhibited α-glucosidase inhibition. All extracts exhibited a strong anti-tyrosinase activity, which was further investigated by molecular docking and molecular dynamic. After 48 h exposure to the extracts for HeLa cell lines, the ethyl acetate-Soxhlet extract showed the highest inhibition (IC50 = 533.1 μg/mL) while the decoction extract was more cytotoxic to MDA-MB-231 cells (IC50 = 337.4 μg/mL). Treatment of cancer cell lines with all P. phillyreifolius extracts resulted in a time-dependent reduction of cell viability for HeLa and dose-and time-dependent reduction for MDA-MB-231. Gene expression ratio of Bcl-2 to Bax was higher for all Soxhlet-extracts. Total phenolics (TPC) and flavonoids (TFC) content were highest in the decoction and methanol-Soxhlet extract, respectively (122.43 mg GAE/g extract and 31.28 mg RE/g extract, respectively). The extracts were abundant in ellagitannins, although phenolic acids and flavonoids were also detected. Granatin B was detected for the first time in Phyllanthus species. Overall, the aerial parts of P. phillyreifolius exemplify a potent reservoir of bioactive phytochemicals for therapeutic applications.

Authors+Show Affiliations

Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit 230, Mauritius.Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey.Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, Spain.Department of Biology, Faculty of Science and Arts, Kastamonu University, Kastamonu, Turkey.Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey. Electronic address: mcbaloglu@kastamonu.edu.tr.Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey.Department of Pharmacy, University "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.Department of Agriculture and Food Science, Faculty of Agriculture, University of Mauritius, 230 Réduit, Mauritius.Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit 230, Mauritius.Department of Agriculture and Food Science, Faculty of Agriculture, University of Mauritius, 230 Réduit, Mauritius.Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30599961

Citation

Mahomoodally, Mohamad Fawzi, et al. "Pharmacological and Polyphenolic Profiles of Phyllanthus Phillyreifolius Var. Commersonii Müll. Arg: an Unexplored Endemic Species From Mauritius." Food Research International (Ottawa, Ont.), vol. 115, 2019, pp. 425-438.
Mahomoodally MF, Yerlikaya S, Llorent-Martínez EJ, et al. Pharmacological and polyphenolic profiles of Phyllanthus phillyreifolius var. commersonii Müll. Arg: An unexplored endemic species from Mauritius. Food Res Int. 2019;115:425-438.
Mahomoodally, M. F., Yerlikaya, S., Llorent-Martínez, E. J., Uğurlu, A., Baloglu, M. C., Altunoglu, Y. C., Mollica, A., Dardenne, K. K., Aumeeruddy, M. Z., Puchooa, D., & Zengin, G. (2019). Pharmacological and polyphenolic profiles of Phyllanthus phillyreifolius var. commersonii Müll. Arg: An unexplored endemic species from Mauritius. Food Research International (Ottawa, Ont.), 115, 425-438. https://doi.org/10.1016/j.foodres.2018.10.075
Mahomoodally MF, et al. Pharmacological and Polyphenolic Profiles of Phyllanthus Phillyreifolius Var. Commersonii Müll. Arg: an Unexplored Endemic Species From Mauritius. Food Res Int. 2019;115:425-438. PubMed PMID: 30599961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological and polyphenolic profiles of Phyllanthus phillyreifolius var. commersonii Müll. Arg: An unexplored endemic species from Mauritius. AU - Mahomoodally,Mohamad Fawzi, AU - Yerlikaya,Serife, AU - Llorent-Martínez,Eulogio J, AU - Uğurlu,Asli, AU - Baloglu,Mehmet Cengiz, AU - Altunoglu,Yasemin Celik, AU - Mollica,Adriano, AU - Dardenne,Kurty Kersley, AU - Aumeeruddy,Muhammad Zakariyyah, AU - Puchooa,Daneshwar, AU - Zengin,Gokhan, Y1 - 2018/10/28/ PY - 2018/09/08/received PY - 2018/10/22/revised PY - 2018/10/25/accepted PY - 2019/1/3/entrez PY - 2019/1/3/pubmed PY - 2020/1/7/medline KW - Antibacterial KW - Anticancer KW - Antioxidant KW - Enzyme inhibition KW - Phyllanthus phillyreifolius KW - Polyphenolic SP - 425 EP - 438 JF - Food research international (Ottawa, Ont.) JO - Food Res Int VL - 115 N2 - Phyllanthus phillyreifolius var. commersonii Müll. Arg is an endemic plant of Mauritius. To date, no study has been performed concerning its polyphenolic profile and pharmacological properties. In this study, a decoction (water), ethyl acetate and methanol extracts of the aerial parts of P. phillyreifolius, obtained from different extraction procedures (maceration and Soxhlet), were studied for antibacterial, antioxidant, anticancer, and enzyme inhibitory properties along with their polyphenolic profile. The ethyl acetate macerated extract showed high antibacterial activity against B. cereus (MIC = 0.293 mg/mL) and E. coli (MIC = 0.417 mg/mL) while S. epidermidis was most susceptible to the ethyl acetate-Soxhlet extract (MIC = 0.521 mg/mL). The methanol-Soxhlet extract displayed the most potent cupric and ferric reducing power, and metal chelating effect, while the macerated methanolic extract was the most effective DPPH and ABTS scavenger, and BChE inhibitor. Only the ethyl acetate-Soxhlet extract exhibited α-glucosidase inhibition. All extracts exhibited a strong anti-tyrosinase activity, which was further investigated by molecular docking and molecular dynamic. After 48 h exposure to the extracts for HeLa cell lines, the ethyl acetate-Soxhlet extract showed the highest inhibition (IC50 = 533.1 μg/mL) while the decoction extract was more cytotoxic to MDA-MB-231 cells (IC50 = 337.4 μg/mL). Treatment of cancer cell lines with all P. phillyreifolius extracts resulted in a time-dependent reduction of cell viability for HeLa and dose-and time-dependent reduction for MDA-MB-231. Gene expression ratio of Bcl-2 to Bax was higher for all Soxhlet-extracts. Total phenolics (TPC) and flavonoids (TFC) content were highest in the decoction and methanol-Soxhlet extract, respectively (122.43 mg GAE/g extract and 31.28 mg RE/g extract, respectively). The extracts were abundant in ellagitannins, although phenolic acids and flavonoids were also detected. Granatin B was detected for the first time in Phyllanthus species. Overall, the aerial parts of P. phillyreifolius exemplify a potent reservoir of bioactive phytochemicals for therapeutic applications. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/30599961/Pharmacological_and_polyphenolic_profiles_of_Phyllanthus_phillyreifolius_var__commersonii_Müll__Arg:_An_unexplored_endemic_species_from_Mauritius_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0963-9969(18)30867-6 DB - PRIME DP - Unbound Medicine ER -