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Palindromic Molecular Beacon Based Z-Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection.
Anal Chem. 2019 02 05; 91(3):2447-2454.AC

Abstract

This work presented an innovative and rationally engineered palindromic molecular beacon (PMB) based "Z-scheme" photoelectrochemical (PEC) biosensing protocol for the selective screening of kanamycin (Kana) through DNA hybridization-induced conformational conversion. Interestingly, the ingeniously designed PMB integrated the multifunctional elements including recognition region, primer-like palindromic fragment, and polymerization-nicking template. The cosensitized structures consisted of CdS quantum dot functionalized hairpin DNA2 (QD-HP2) and region-selectively deposited gold nanoparticles onto {001} facets of bismuth oxychloride (BiOCl-Au). Compared with BiOCl-Au alone, the attachment of CdS QDs onto BiOCl-Au (i.e., BiOCl-Au-CdS QDs) exhibited evidently enhanced photocurrent intensity thanks to the synergistic effect of Z-scheme BiOCl-Au-CdS QDs. After incubation with target Kana, Kana-aptamer binding could induce the exposure of PMB region for hairpin DNA1 (HP1). The exposed palindromic tails hybridized with each other (like a molecular machine) to consume the substrates (dNTPs) and fuels (enzyme) for the releasing of numerous nick fragments (Nick). The as-generated nick fragments could specifically hybridize with the complementary region of QD-HP2, thus resulting in decreasing photocurrent because of the increasing spatial distance for electron transfer between two-type photosensitizers. Under optimum conditions, the PMB-based sensing system exhibited satisfying photocurrent responses toward target Kana within the working range from 50 to 5000 fM at a low detection limit of 29 fM. Impressively, the concept of a palindromic fragment-mediated primer-free biosensing strategy offers a new avenue for advanced development of efficient and convenient biodetection systems.

Authors+Show Affiliations

Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.Chair for Analytical Chemistry and Water Chemistry, Institute of Hydrochemistry , Technische Universität München , Marchioninistrasse 17 , D-81377 München , Germany.Chair for Analytical Chemistry and Water Chemistry, Institute of Hydrochemistry , Technische Universität München , Marchioninistrasse 17 , D-81377 München , Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30609356

Citation

Zeng, Ruijin, et al. "Palindromic Molecular Beacon Based Z-Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection." Analytical Chemistry, vol. 91, no. 3, 2019, pp. 2447-2454.
Zeng R, Luo Z, Su L, et al. Palindromic Molecular Beacon Based Z-Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection. Anal Chem. 2019;91(3):2447-2454.
Zeng, R., Luo, Z., Su, L., Zhang, L., Tang, D., Niessner, R., & Knopp, D. (2019). Palindromic Molecular Beacon Based Z-Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection. Analytical Chemistry, 91(3), 2447-2454. https://doi.org/10.1021/acs.analchem.8b05265
Zeng R, et al. Palindromic Molecular Beacon Based Z-Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection. Anal Chem. 2019 02 5;91(3):2447-2454. PubMed PMID: 30609356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Palindromic Molecular Beacon Based Z-Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection. AU - Zeng,Ruijin, AU - Luo,Zhongbin, AU - Su,Lingshan, AU - Zhang,Lijia, AU - Tang,Dianping, AU - Niessner,Reinhard, AU - Knopp,Dietmar, Y1 - 2019/01/16/ PY - 2019/1/5/pubmed PY - 2020/7/31/medline PY - 2019/1/5/entrez SP - 2447 EP - 2454 JF - Analytical chemistry JO - Anal Chem VL - 91 IS - 3 N2 - This work presented an innovative and rationally engineered palindromic molecular beacon (PMB) based "Z-scheme" photoelectrochemical (PEC) biosensing protocol for the selective screening of kanamycin (Kana) through DNA hybridization-induced conformational conversion. Interestingly, the ingeniously designed PMB integrated the multifunctional elements including recognition region, primer-like palindromic fragment, and polymerization-nicking template. The cosensitized structures consisted of CdS quantum dot functionalized hairpin DNA2 (QD-HP2) and region-selectively deposited gold nanoparticles onto {001} facets of bismuth oxychloride (BiOCl-Au). Compared with BiOCl-Au alone, the attachment of CdS QDs onto BiOCl-Au (i.e., BiOCl-Au-CdS QDs) exhibited evidently enhanced photocurrent intensity thanks to the synergistic effect of Z-scheme BiOCl-Au-CdS QDs. After incubation with target Kana, Kana-aptamer binding could induce the exposure of PMB region for hairpin DNA1 (HP1). The exposed palindromic tails hybridized with each other (like a molecular machine) to consume the substrates (dNTPs) and fuels (enzyme) for the releasing of numerous nick fragments (Nick). The as-generated nick fragments could specifically hybridize with the complementary region of QD-HP2, thus resulting in decreasing photocurrent because of the increasing spatial distance for electron transfer between two-type photosensitizers. Under optimum conditions, the PMB-based sensing system exhibited satisfying photocurrent responses toward target Kana within the working range from 50 to 5000 fM at a low detection limit of 29 fM. Impressively, the concept of a palindromic fragment-mediated primer-free biosensing strategy offers a new avenue for advanced development of efficient and convenient biodetection systems. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/30609356/Palindromic_Molecular_Beacon_Based_Z_Scheme_BiOCl_Au_CdS_Photoelectrochemical_Biodetection_ L2 - https://doi.org/10.1021/acs.analchem.8b05265 DB - PRIME DP - Unbound Medicine ER -