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NADPH Oxidase (Rboh) Activity is Up Regulated during Sweet Pepper (Capsicum annuum L.) Fruit Ripening.
Antioxidants (Basel). 2019 Jan 01; 8(1)A

Abstract

In plants, NADPH oxidase (NOX) is also known as a respiratory burst oxidase homolog (Rboh). This highly important enzyme, one of the main enzymatic sources of superoxide radicals (O₂•-), is involved in the metabolism of reactive oxygen and nitrogen species (ROS and RNS), which is active in the non-climacteric pepper (Capsicum annuum L.) fruit. We used sweet pepper fruits at two ripening stages (green and red) to biochemically analyze the O₂•--generating Rboh activity and the number of isozymes during this physiological process. Malondialdehyde (MDA) content, an oxidative stress marker, was also assayed as an index of lipid peroxidation. In red fruits, MDA was observed to increase 2-fold accompanied by a 5.3-fold increase in total Rboh activity. Using in-gel assays of Rboh activity, we identified a total of seven CaRboh isozymes (I⁻VII) which were differentially modulated during ripening. CaRboh-III and CaRboh-I were the most prominent isozymes in green and red fruits, respectively. An in vitro assay showed that CaRboh activity is inhibited in the presence of nitric oxide (NO) donors, peroxynitrite (ONOO-) and glutathione (GSH), suggesting that CaRboh can undergo S-nitrosation, Tyr-nitration, and glutathionylation, respectively. In summary, this study provides a basic biochemical characterization of CaRboh activity in pepper fruits and indicates that this O₂•--generating Rboh is involved in nitro-oxidative stress associated with sweet pepper fruit ripening.

Authors+Show Affiliations

Group Antioxidant, Free Radical and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain. angelachupuga@gmail.com.Group Antioxidant, Free Radical and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain. salvador.gonzalez@eez.csic.es.Group Antioxidant, Free Radical and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain. martarodriguezruiz@usp.br.Group Antioxidant, Free Radical and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain. josemanuel.palma@eez.csic.es.Group Antioxidant, Free Radical and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain. javier.corpas@eez.csic.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30609654

Citation

Chu-Puga, Ángela, et al. "NADPH Oxidase (Rboh) Activity Is Up Regulated During Sweet Pepper (Capsicum Annuum L.) Fruit Ripening." Antioxidants (Basel, Switzerland), vol. 8, no. 1, 2019.
Chu-Puga Á, González-Gordo S, Rodríguez-Ruiz M, et al. NADPH Oxidase (Rboh) Activity is Up Regulated during Sweet Pepper (Capsicum annuum L.) Fruit Ripening. Antioxidants (Basel). 2019;8(1).
Chu-Puga, Á., González-Gordo, S., Rodríguez-Ruiz, M., Palma, J. M., & Corpas, F. J. (2019). NADPH Oxidase (Rboh) Activity is Up Regulated during Sweet Pepper (Capsicum annuum L.) Fruit Ripening. Antioxidants (Basel, Switzerland), 8(1). https://doi.org/10.3390/antiox8010009
Chu-Puga Á, et al. NADPH Oxidase (Rboh) Activity Is Up Regulated During Sweet Pepper (Capsicum Annuum L.) Fruit Ripening. Antioxidants (Basel). 2019 Jan 1;8(1) PubMed PMID: 30609654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NADPH Oxidase (Rboh) Activity is Up Regulated during Sweet Pepper (Capsicum annuum L.) Fruit Ripening. AU - Chu-Puga,Ángela, AU - González-Gordo,Salvador, AU - Rodríguez-Ruiz,Marta, AU - Palma,José M, AU - Corpas,Francisco J, Y1 - 2019/01/01/ PY - 2018/11/05/received PY - 2018/12/06/revised PY - 2018/12/25/accepted PY - 2019/1/6/entrez PY - 2019/1/6/pubmed PY - 2019/1/6/medline KW - NADPH oxidase KW - S-nitrosation KW - Tyr-nitration KW - nitration KW - nitric oxide KW - pepper fruit KW - peroxynitrite KW - respiratory burst oxidase homolog (Rboh) KW - ripening JF - Antioxidants (Basel, Switzerland) JO - Antioxidants (Basel) VL - 8 IS - 1 N2 - In plants, NADPH oxidase (NOX) is also known as a respiratory burst oxidase homolog (Rboh). This highly important enzyme, one of the main enzymatic sources of superoxide radicals (O₂•-), is involved in the metabolism of reactive oxygen and nitrogen species (ROS and RNS), which is active in the non-climacteric pepper (Capsicum annuum L.) fruit. We used sweet pepper fruits at two ripening stages (green and red) to biochemically analyze the O₂•--generating Rboh activity and the number of isozymes during this physiological process. Malondialdehyde (MDA) content, an oxidative stress marker, was also assayed as an index of lipid peroxidation. In red fruits, MDA was observed to increase 2-fold accompanied by a 5.3-fold increase in total Rboh activity. Using in-gel assays of Rboh activity, we identified a total of seven CaRboh isozymes (I⁻VII) which were differentially modulated during ripening. CaRboh-III and CaRboh-I were the most prominent isozymes in green and red fruits, respectively. An in vitro assay showed that CaRboh activity is inhibited in the presence of nitric oxide (NO) donors, peroxynitrite (ONOO-) and glutathione (GSH), suggesting that CaRboh can undergo S-nitrosation, Tyr-nitration, and glutathionylation, respectively. In summary, this study provides a basic biochemical characterization of CaRboh activity in pepper fruits and indicates that this O₂•--generating Rboh is involved in nitro-oxidative stress associated with sweet pepper fruit ripening. SN - 2076-3921 UR - https://www.unboundmedicine.com/medline/citation/30609654/NADPH_Oxidase__Rboh__Activity_is_Up_Regulated_during_Sweet_Pepper__Capsicum_annuum_L___Fruit_Ripening_ L2 - https://www.mdpi.com/resolver?pii=antiox8010009 DB - PRIME DP - Unbound Medicine ER -
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