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Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells.
J Microbiol Biotechnol. 2019 Feb 28; 29(2):321-329.JM

Abstract

Hair loss, also known as alopecia, is a common dermatological condition of psychosocial significance; development of therapeutic candidates for the treatment of this condition is, hence, important. Silibinin, a secondary metabolite from Silybum marianum, is an effective antioxidant that also prevents various cutaneous problems. In this study, we have investigated the effect of silibinin on hair induction using three-dimensional (3D) cultured, human dermal papilla (DP) spheroids. Silibinin was found to significantly increase viability through AKT serine/threonine kinase (AKT) activation in 3D DP spheroids. This was correlated with an increase in the diameter of the 3D DP spheroids. The activation of the wingless and INT-1 (Wnt)/β-catenin signaling pathway, which is associated with hair growth induction in the DP, was evaluated using the T cell-specific transcription factor and lymphoid enhancer-binding factor (TCF/LEF) transcription factor reporter assay; results indicated significantly increased luciferase activity. In addition, we were able to demonstrate increased expression of the target genes, WNT5a and LEF1, using quantitative real-time PCR assay. Lastly, significantly elevated expression of signature genes associated with hair induction was demonstrated in the 3D DP spheroids treated with silibinin. These results suggest that silibinin promotes proliferation and hair induction through the AKT and Wnt/β-catenin signaling pathways in 3D DP spheroids. Silibinin can be a potential candidate to promote hair proliferation.

Authors+Show Affiliations

Department of Dermatology, Konkuk University School of Medicine, Seoul 05030, Republic of Korea.Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea.Department of Dermatology, Konkuk University School of Medicine, Seoul 05030, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30609881

Citation

Cheon, Hye In, et al. "Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells." Journal of Microbiology and Biotechnology, vol. 29, no. 2, 2019, pp. 321-329.
Cheon HI, Bae S, Ahn KJ. Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells. J Microbiol Biotechnol. 2019;29(2):321-329.
Cheon, H. I., Bae, S., & Ahn, K. J. (2019). Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells. Journal of Microbiology and Biotechnology, 29(2), 321-329. https://doi.org/10.4014/jmb.1810.10050
Cheon HI, Bae S, Ahn KJ. Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells. J Microbiol Biotechnol. 2019 Feb 28;29(2):321-329. PubMed PMID: 30609881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells. AU - Cheon,Hye In, AU - Bae,Seunghee, AU - Ahn,Kyu Joong, PY - 2019/1/6/pubmed PY - 2019/4/19/medline PY - 2019/1/6/entrez KW - Akt KW - Silibinin KW - dermal papilla cells KW - hair growth inductive KW - spheroid culture SP - 321 EP - 329 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 29 IS - 2 N2 - Hair loss, also known as alopecia, is a common dermatological condition of psychosocial significance; development of therapeutic candidates for the treatment of this condition is, hence, important. Silibinin, a secondary metabolite from Silybum marianum, is an effective antioxidant that also prevents various cutaneous problems. In this study, we have investigated the effect of silibinin on hair induction using three-dimensional (3D) cultured, human dermal papilla (DP) spheroids. Silibinin was found to significantly increase viability through AKT serine/threonine kinase (AKT) activation in 3D DP spheroids. This was correlated with an increase in the diameter of the 3D DP spheroids. The activation of the wingless and INT-1 (Wnt)/β-catenin signaling pathway, which is associated with hair growth induction in the DP, was evaluated using the T cell-specific transcription factor and lymphoid enhancer-binding factor (TCF/LEF) transcription factor reporter assay; results indicated significantly increased luciferase activity. In addition, we were able to demonstrate increased expression of the target genes, WNT5a and LEF1, using quantitative real-time PCR assay. Lastly, significantly elevated expression of signature genes associated with hair induction was demonstrated in the 3D DP spheroids treated with silibinin. These results suggest that silibinin promotes proliferation and hair induction through the AKT and Wnt/β-catenin signaling pathways in 3D DP spheroids. Silibinin can be a potential candidate to promote hair proliferation. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/30609881/Flavonoid_Silibinin_Increases_Hair_Inductive_Property_Via_Akt_and_Wnt/β_Catenin_Signaling_Activation_in_3_Dimensional_Spheroid_Cultured_Human_Dermal_Papilla_Cells_ L2 - http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1810.10050 DB - PRIME DP - Unbound Medicine ER -