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Concurrent exercise improves insulin resistance and nonalcoholic fatty liver disease by upregulating PPAR-γ and genes involved in the beta-oxidation of fatty acids in ApoE-KO mice fed a high-fat diet.
Lipids Health Dis. 2019 Jan 05; 18(1):6.LH

Abstract

OBJECTIVE

To emphasize the mechanism of concurrent exercise effect on lipid disorders in insulin resistance (IR) and nonalcoholic fatty liver disease (NAFLD).

MATERIALS AND METHODS

Twenty male ApoE knockout mice were randomly divided into two groups: HFD group (n = 10) fed a high fat diet, and HFDE group (n = 10) with high-fat diet intervention for 12 weeks and swimming exercise. Other ten healthy male C57BL/6 J mice were fed a normal diet, and included as control group. Retro-orbital blood samples were collected for biochemical analysis. Oil red O staining of liver tissues was performed to confirm the exercise effect. Western blotting was performed to evaluate the expressions of PPAR-γ, CPT-1, MCAD.

RESULTS

The levels of TG, TC, LDL, FFA, FIN, FPG and Homa-IRI in the HFD group were significantly higher than ND group, while these were markedly decreased in the HFDE group compared with HFD group. The Oil Red O staining of liver samples further confirmed the exercise effect on the change of lipid deposition in the liver. Western blotting showed increased expressions of PPAR-γ, CPT-1, MCAD induced by high fat diet were significantly downregulated by exercise.

CONCLUSION

A concurrent 12-week exercise protocol alleviated the lipid metabolism disorders of IR and NAFLD, probably via PPAR-γ/CPT-1/MCAD signaling.

Authors+Show Affiliations

Cardiac Rehabilitation Center of Rehabilitation Department, Xiangya Hospital at Central South University, Xiangya Road 87#, Changsha, Hunan, People's Republic of China.Cardiac Rehabilitation Center of Rehabilitation Department, Xiangya Hospital at Central South University, Xiangya Road 87#, Changsha, Hunan, People's Republic of China. 3386589875@qq.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30611282

Citation

Zheng, Fan, and Ying Cai. "Concurrent Exercise Improves Insulin Resistance and Nonalcoholic Fatty Liver Disease By Upregulating PPAR-γ and Genes Involved in the Beta-oxidation of Fatty Acids in ApoE-KO Mice Fed a High-fat Diet." Lipids in Health and Disease, vol. 18, no. 1, 2019, p. 6.
Zheng F, Cai Y. Concurrent exercise improves insulin resistance and nonalcoholic fatty liver disease by upregulating PPAR-γ and genes involved in the beta-oxidation of fatty acids in ApoE-KO mice fed a high-fat diet. Lipids Health Dis. 2019;18(1):6.
Zheng, F., & Cai, Y. (2019). Concurrent exercise improves insulin resistance and nonalcoholic fatty liver disease by upregulating PPAR-γ and genes involved in the beta-oxidation of fatty acids in ApoE-KO mice fed a high-fat diet. Lipids in Health and Disease, 18(1), 6. https://doi.org/10.1186/s12944-018-0933-z
Zheng F, Cai Y. Concurrent Exercise Improves Insulin Resistance and Nonalcoholic Fatty Liver Disease By Upregulating PPAR-γ and Genes Involved in the Beta-oxidation of Fatty Acids in ApoE-KO Mice Fed a High-fat Diet. Lipids Health Dis. 2019 Jan 5;18(1):6. PubMed PMID: 30611282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Concurrent exercise improves insulin resistance and nonalcoholic fatty liver disease by upregulating PPAR-γ and genes involved in the beta-oxidation of fatty acids in ApoE-KO mice fed a high-fat diet. AU - Zheng,Fan, AU - Cai,Ying, Y1 - 2019/01/05/ PY - 2018/03/31/received PY - 2018/11/27/accepted PY - 2019/1/7/entrez PY - 2019/1/7/pubmed PY - 2019/3/19/medline KW - CPT-1 KW - Concurrent exercise KW - Insulin resistance KW - MCAD KW - Non-alcoholic fatty liver disease KW - PPAR-γ SP - 6 EP - 6 JF - Lipids in health and disease JO - Lipids Health Dis VL - 18 IS - 1 N2 - OBJECTIVE: To emphasize the mechanism of concurrent exercise effect on lipid disorders in insulin resistance (IR) and nonalcoholic fatty liver disease (NAFLD). MATERIALS AND METHODS: Twenty male ApoE knockout mice were randomly divided into two groups: HFD group (n = 10) fed a high fat diet, and HFDE group (n = 10) with high-fat diet intervention for 12 weeks and swimming exercise. Other ten healthy male C57BL/6 J mice were fed a normal diet, and included as control group. Retro-orbital blood samples were collected for biochemical analysis. Oil red O staining of liver tissues was performed to confirm the exercise effect. Western blotting was performed to evaluate the expressions of PPAR-γ, CPT-1, MCAD. RESULTS: The levels of TG, TC, LDL, FFA, FIN, FPG and Homa-IRI in the HFD group were significantly higher than ND group, while these were markedly decreased in the HFDE group compared with HFD group. The Oil Red O staining of liver samples further confirmed the exercise effect on the change of lipid deposition in the liver. Western blotting showed increased expressions of PPAR-γ, CPT-1, MCAD induced by high fat diet were significantly downregulated by exercise. CONCLUSION: A concurrent 12-week exercise protocol alleviated the lipid metabolism disorders of IR and NAFLD, probably via PPAR-γ/CPT-1/MCAD signaling. SN - 1476-511X UR - https://www.unboundmedicine.com/medline/citation/30611282/Concurrent_exercise_improves_insulin_resistance_and_nonalcoholic_fatty_liver_disease_by_upregulating_PPAR_��_and_genes_involved_in_the_beta_oxidation_of_fatty_acids_in_ApoE_KO_mice_fed_a_high_fat_diet_ L2 - https://lipidworld.biomedcentral.com/articles/10.1186/s12944-018-0933-z DB - PRIME DP - Unbound Medicine ER -