Tags

Type your tag names separated by a space and hit enter

Influences of zinc oxide nanoparticles on Allium cepa root cells and the primary cause of phytotoxicity.
Ecotoxicology. 2019 Mar; 28(2):175-188.E

Abstract

Zinc oxide nanoparticles (ZnO-NPs) are widely used in consumer products, which have raised concerns about their impact on the human health and environment. In this study, Allium cepa were treated with 5 and 50 μg/mL ZnO-NPs solutions for 12, 24, and 36 h, respectively. The cytotoxic and genotoxic effects of ZnO-NPs in root meristems of Allium cepa cells were characterized by cell membrane integrity, metabolic activity, reactive oxygen species (ROS) accumulation, DNA damage, chromosome aberration, and cell cycle progression. Substantially elevated Zn levels were observed in the cytoplasmic and nuclear fractions, and the accumulation of zinc in the nuclear fraction (up to 9764 μg/g) was one magnitude greater than that in the cytoplasm (up to 541 μg/g). The complexation of Zn2+ with diethylene triamine pentacetic acid (DTPA) was performed to explicate the respective contribution of insoluble particles or Zn2+ to ZnO-NPs toxicity. We found that the inhibition of root growth accounted for 24.2% or 36.1% when the plants were exposed to Zn2+ that released from 5 or 50 μg/mL of ZnO-NPs for 36 h, respectively, whereas the exposure to 5 or 50 μg/mL of insoluble particles resulted in 75.8% or 63.9% of inhibition, respectively. These findings demonstrated that adverse effects exerted not just by Zn2+ released from ZnO-NPs, but also directly from the nanoparticles. These findings contribute to a better understanding of ZnO-NPs cytotoxicity and genotoxicity in plant cells and provide valuable information for further research on the phytotoxic mechanisms of ZnO-NPs.

Authors+Show Affiliations

Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, China.Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, China.Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, China.Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, China.School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, 510632, Guangzhou, China.Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, China. lishsh@scnu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30612257

Citation

Sun, Zhiqiang, et al. "Influences of Zinc Oxide Nanoparticles On Allium Cepa Root Cells and the Primary Cause of Phytotoxicity." Ecotoxicology (London, England), vol. 28, no. 2, 2019, pp. 175-188.
Sun Z, Xiong T, Zhang T, et al. Influences of zinc oxide nanoparticles on Allium cepa root cells and the primary cause of phytotoxicity. Ecotoxicology. 2019;28(2):175-188.
Sun, Z., Xiong, T., Zhang, T., Wang, N., Chen, D., & Li, S. (2019). Influences of zinc oxide nanoparticles on Allium cepa root cells and the primary cause of phytotoxicity. Ecotoxicology (London, England), 28(2), 175-188. https://doi.org/10.1007/s10646-018-2010-9
Sun Z, et al. Influences of Zinc Oxide Nanoparticles On Allium Cepa Root Cells and the Primary Cause of Phytotoxicity. Ecotoxicology. 2019;28(2):175-188. PubMed PMID: 30612257.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influences of zinc oxide nanoparticles on Allium cepa root cells and the primary cause of phytotoxicity. AU - Sun,Zhiqiang, AU - Xiong,Tiantian, AU - Zhang,Ting, AU - Wang,Nanfang, AU - Chen,Da, AU - Li,Shaoshan, Y1 - 2019/01/05/ PY - 2018/12/10/accepted PY - 2019/1/7/pubmed PY - 2019/5/28/medline PY - 2019/1/7/entrez KW - Allium cepa KW - DTPA KW - Phytotoxicity KW - Zinc oxide nanoparticles SP - 175 EP - 188 JF - Ecotoxicology (London, England) JO - Ecotoxicology VL - 28 IS - 2 N2 - Zinc oxide nanoparticles (ZnO-NPs) are widely used in consumer products, which have raised concerns about their impact on the human health and environment. In this study, Allium cepa were treated with 5 and 50 μg/mL ZnO-NPs solutions for 12, 24, and 36 h, respectively. The cytotoxic and genotoxic effects of ZnO-NPs in root meristems of Allium cepa cells were characterized by cell membrane integrity, metabolic activity, reactive oxygen species (ROS) accumulation, DNA damage, chromosome aberration, and cell cycle progression. Substantially elevated Zn levels were observed in the cytoplasmic and nuclear fractions, and the accumulation of zinc in the nuclear fraction (up to 9764 μg/g) was one magnitude greater than that in the cytoplasm (up to 541 μg/g). The complexation of Zn2+ with diethylene triamine pentacetic acid (DTPA) was performed to explicate the respective contribution of insoluble particles or Zn2+ to ZnO-NPs toxicity. We found that the inhibition of root growth accounted for 24.2% or 36.1% when the plants were exposed to Zn2+ that released from 5 or 50 μg/mL of ZnO-NPs for 36 h, respectively, whereas the exposure to 5 or 50 μg/mL of insoluble particles resulted in 75.8% or 63.9% of inhibition, respectively. These findings demonstrated that adverse effects exerted not just by Zn2+ released from ZnO-NPs, but also directly from the nanoparticles. These findings contribute to a better understanding of ZnO-NPs cytotoxicity and genotoxicity in plant cells and provide valuable information for further research on the phytotoxic mechanisms of ZnO-NPs. SN - 1573-3017 UR - https://www.unboundmedicine.com/medline/citation/30612257/Influences_of_zinc_oxide_nanoparticles_on_Allium_cepa_root_cells_and_the_primary_cause_of_phytotoxicity_ L2 - https://doi.org/10.1007/s10646-018-2010-9 DB - PRIME DP - Unbound Medicine ER -