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Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array.
Anal Chim Acta. 2019 Feb 21; 1049:213-218.AC

Abstract

Quantitative detection of multiple chicken cytokines is a good evaluation of cell-mediated immunity in chickens after disease infection or vaccination. However, current assay methods for chicken cytokines cannot meet the needs of clinical diagnosis due to unsatisfactory sensitivity and low assay throughput. Herein, a sensitive chemiluminescence (CL) imaging immunosensor array has been developed for high-throughput detection of multiple chicken cytokines. The chicken cytokines immunosensor array was prepared by assembling different cytokine capture antibodies onto a disposable silanized glass chip, where horseradish peroxidase and antibody-conjugated gold nanoparticles were used as multienzymatic amplification probe for CL imaging signal amplification. By using a sandwich assay mode, the amplified CL signals from each sensing array cell were collected for quantitation. Using chicken interleukin-4 and chicken interferon-γ as model cytokines, this novel multiplexed and amplified method demonstrated simultaneous measurement of the two chicken cytokines in the linear ranges of 0.008-0.12 ng/mL and 0.005-0.20 ng/mL, respectively, which yields limits of detection down to 2 pg/mL and 3 pg/mL. The CL imaging array method reported here also demonstrated high specificity, good repeatability, and high stability and accuracy, providing a novel multiplex immunoassay strategy for highly sensitive and high-throughput detection of chicken cytokines and further disease diagnosis in poultry.

Authors+Show Affiliations

Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.Department of Laboratory Medicine and Clinical Medical College of Yangzhou University, Subei Peoples' Hospital of Jiangsu Province, Yangzhou, 225001, PR China.Department of Laboratory Medicine and Clinical Medical College of Yangzhou University, Subei Peoples' Hospital of Jiangsu Province, Yangzhou, 225001, PR China.Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.Environmental Toxicology and Department of Chemistry, University of California, Riverside, CA, 92521, United States.Environmental Toxicology and Department of Chemistry, University of California, Riverside, CA, 92521, United States. Electronic address: quan.cheng@ucr.edu.Guangling College, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China. Electronic address: zjyang@yzu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30612653

Citation

Zhong, Yihong, et al. "Multiplex Immunoassay of Chicken Cytokines Via Highly-sensitive Chemiluminescent Imaging Array." Analytica Chimica Acta, vol. 1049, 2019, pp. 213-218.
Zhong Y, Wu X, Li J, et al. Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array. Anal Chim Acta. 2019;1049:213-218.
Zhong, Y., Wu, X., Li, J., Lan, Q., Jing, Q., Min, L., Ren, C., Hu, X., Lambert, A., Cheng, Q., & Yang, Z. (2019). Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array. Analytica Chimica Acta, 1049, 213-218. https://doi.org/10.1016/j.aca.2018.10.050
Zhong Y, et al. Multiplex Immunoassay of Chicken Cytokines Via Highly-sensitive Chemiluminescent Imaging Array. Anal Chim Acta. 2019 Feb 21;1049:213-218. PubMed PMID: 30612653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array. AU - Zhong,Yihong, AU - Wu,Xinyue, AU - Li,Juan, AU - Lan,Qingchun, AU - Jing,Qingling, AU - Min,Lingfeng, AU - Ren,Chuanli, AU - Hu,Xiaoya, AU - Lambert,Alexander, AU - Cheng,Quan, AU - Yang,Zhanjun, Y1 - 2018/10/25/ PY - 2018/09/14/received PY - 2018/10/20/revised PY - 2018/10/23/accepted PY - 2019/1/8/entrez PY - 2019/1/8/pubmed PY - 2019/3/6/medline KW - Chemiluminescence imaging KW - Cytokines KW - Multiplex immunoassay KW - Nanoparticle-signal amplification KW - Sensor array SP - 213 EP - 218 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 1049 N2 - Quantitative detection of multiple chicken cytokines is a good evaluation of cell-mediated immunity in chickens after disease infection or vaccination. However, current assay methods for chicken cytokines cannot meet the needs of clinical diagnosis due to unsatisfactory sensitivity and low assay throughput. Herein, a sensitive chemiluminescence (CL) imaging immunosensor array has been developed for high-throughput detection of multiple chicken cytokines. The chicken cytokines immunosensor array was prepared by assembling different cytokine capture antibodies onto a disposable silanized glass chip, where horseradish peroxidase and antibody-conjugated gold nanoparticles were used as multienzymatic amplification probe for CL imaging signal amplification. By using a sandwich assay mode, the amplified CL signals from each sensing array cell were collected for quantitation. Using chicken interleukin-4 and chicken interferon-γ as model cytokines, this novel multiplexed and amplified method demonstrated simultaneous measurement of the two chicken cytokines in the linear ranges of 0.008-0.12 ng/mL and 0.005-0.20 ng/mL, respectively, which yields limits of detection down to 2 pg/mL and 3 pg/mL. The CL imaging array method reported here also demonstrated high specificity, good repeatability, and high stability and accuracy, providing a novel multiplex immunoassay strategy for highly sensitive and high-throughput detection of chicken cytokines and further disease diagnosis in poultry. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/30612653/Multiplex_immunoassay_of_chicken_cytokines_via_highly_sensitive_chemiluminescent_imaging_array_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(18)31292-3 DB - PRIME DP - Unbound Medicine ER -