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L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation.
Plant Mol Biol 2019; 99(3):283-298PM

Abstract

KEY MESSAGE

Methane-triggered lateral root formation is not only a universal event, but also dependent on L-cysteine desulfhydrase-dependent hydrogen sulfide signaling. Whether or how methane (CH4) triggers lateral root (LR) formation has not been elucidated. In this report, CH4 induction of lateral rooting and the role of hydrogen sulfide (H2S) were dissected in tomato and Arabidopsis by using physiological, anatomical, molecular, and genetic approaches. First, we discovered that CH4 induction of lateral rooting is a universal event. Exogenously applied CH4 not only triggered tomato lateral rooting, but also increased activities of L-cysteine desulfhydrase (DES; a major synthetic enzyme of H2S) and induced endogenous H2S production, and contrasting responses were observed in the presence of hypotaurine (HT; a scavenger of H2S) or DL-propargylglycine (PAG; an inhibitor of DES) alone. CH4-triggered lateral rooting were sensitive to the inhibition of endogenous H2S with HT or PAG. The changes in the transcripts of representative cell cycle regulatory genes, miRNA and its target genes were matched with above phenotypes. In the presence of CH4, Arabidopsis mutant Atdes1 exhibited defects in lateral rooting, compared with the wild-type. Molecular evidence showed that the transcriptional profiles of representative target genes modulated by CH4 in wild-type plants were impaired in Atdes1 mutant. Overall, our data demonstrate the main branch of the DES-dependent H2S signaling cascade in CH4-triggered LR formation.

Authors+Show Affiliations

College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.College of Sciences, Nanjing Agricultural University, Nanjing, 210095, China.College of Sciences, Nanjing Agricultural University, Nanjing, 210095, China.College of Sciences, Nanjing Agricultural University, Nanjing, 210095, China.National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. wbshenh@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30623274

Citation

Mei, Yudong, et al. "L-Cysteine Desulfhydrase-dependent Hydrogen Sulfide Is Required for Methane-induced Lateral Root Formation." Plant Molecular Biology, vol. 99, no. 3, 2019, pp. 283-298.
Mei Y, Zhao Y, Jin X, et al. L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation. Plant Mol Biol. 2019;99(3):283-298.
Mei, Y., Zhao, Y., Jin, X., Wang, R., Xu, N., Hu, J., ... Shen, W. (2019). L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation. Plant Molecular Biology, 99(3), pp. 283-298. doi:10.1007/s11103-018-00817-3.
Mei Y, et al. L-Cysteine Desulfhydrase-dependent Hydrogen Sulfide Is Required for Methane-induced Lateral Root Formation. Plant Mol Biol. 2019;99(3):283-298. PubMed PMID: 30623274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation. AU - Mei,Yudong, AU - Zhao,Yingying, AU - Jin,Xinxin, AU - Wang,Ren, AU - Xu,Na, AU - Hu,Jiawen, AU - Huang,Liqin, AU - Guan,Rongzhan, AU - Shen,Wenbiao, Y1 - 2019/01/08/ PY - 2018/08/28/received PY - 2018/12/20/accepted PY - 2019/1/10/pubmed PY - 2019/3/21/medline PY - 2019/1/10/entrez KW - Arabidopsis KW - Hydrogen sulfide KW - L-cysteine desulfhydrase KW - Lateral root formation KW - Methane KW - Tomato SP - 283 EP - 298 JF - Plant molecular biology JO - Plant Mol. Biol. VL - 99 IS - 3 N2 - KEY MESSAGE: Methane-triggered lateral root formation is not only a universal event, but also dependent on L-cysteine desulfhydrase-dependent hydrogen sulfide signaling. Whether or how methane (CH4) triggers lateral root (LR) formation has not been elucidated. In this report, CH4 induction of lateral rooting and the role of hydrogen sulfide (H2S) were dissected in tomato and Arabidopsis by using physiological, anatomical, molecular, and genetic approaches. First, we discovered that CH4 induction of lateral rooting is a universal event. Exogenously applied CH4 not only triggered tomato lateral rooting, but also increased activities of L-cysteine desulfhydrase (DES; a major synthetic enzyme of H2S) and induced endogenous H2S production, and contrasting responses were observed in the presence of hypotaurine (HT; a scavenger of H2S) or DL-propargylglycine (PAG; an inhibitor of DES) alone. CH4-triggered lateral rooting were sensitive to the inhibition of endogenous H2S with HT or PAG. The changes in the transcripts of representative cell cycle regulatory genes, miRNA and its target genes were matched with above phenotypes. In the presence of CH4, Arabidopsis mutant Atdes1 exhibited defects in lateral rooting, compared with the wild-type. Molecular evidence showed that the transcriptional profiles of representative target genes modulated by CH4 in wild-type plants were impaired in Atdes1 mutant. Overall, our data demonstrate the main branch of the DES-dependent H2S signaling cascade in CH4-triggered LR formation. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/30623274/L-Cysteine_desulfhydrase-dependent_hydrogen_sulfide_is_required_for_methane-induced_lateral_root_formation L2 - https://doi.org/10.1007/s11103-018-00817-3 DB - PRIME DP - Unbound Medicine ER -