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Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury.
Nutrients. 2019 Jan 08; 11(1)N

Abstract

This study was designed to investigate the hepatoprotective effect of bamboo stems using in vitro and in vivo experimental liver damage models. Ethyl acetate fraction of 80% ethanol extract of Phyllostachys nigra stem (PN3) containing polyphenols had a higher NQO1-ARE reporter gene activity as monitored by the activity of the NF-E2-related factor (Nrf2) antioxidant pathway in cells in comparison to extracts from other species and under other conditions. The Nrf2 was translocated from the cytosol to the nucleus in response to PN3, followed by induction of the Nrf2 target gene expression, including HO-1, GCL, and NQO-1 in HepG2 cells. Phosphorylation of Nrf2 in HepG2 cells was enhanced in PN3, which was mediated by PKCδ, ERK, and p38 MAPK. Consequently, PN3 inhibited arachidonic acid (AA) + iron-induced reactive oxygen species generation and glutathione depletion, and, thus, highlighted their role in cytotoxicity. Treatment with major polyphenols of PN3, including catechin, chlorogenic acid, caffeic acid, and p-coumaric acid, also improved AA + iron-mediated oxidative stress and, thus, improved cell viability. Treatment with phenylhydrazine in mice, i.e., the iron overload liver injury model, increased plasma alanine aminotransferase and aspartate aminotransferase levels and changed histological features in mice-a response that was almost completely blocked by PN3 administration. Moreover, PN3 extract mitigated phenylhydrazine-induced oxidative stress and inflammatory responses. Conclusively, PN3 can exert a hepatoprotective effect against iron overload-induced acute liver damage due to its antioxidant properties.

Authors+Show Affiliations

Laboratory of Toxicology, College of Pharmacy, Chosun University, Gwangju 61452, Korea. uranus2k@nate.com. College of Korean Medicine, Dongshin University, Naju, Jeollanam-do 58245, Korea. uranus2k@nate.com.Department of Biochemical and Polymer Engineering, Chosun University, Gwangju 61452, Korea. aamoony1222@naver.com.College of Korean Medicine, Dongshin University, Naju, Jeollanam-do 58245, Korea. nakugi@hanmail.net.Laboratory of Toxicology, College of Pharmacy, Chosun University, Gwangju 61452, Korea. samseok7@gmail.com.Laboratory of Toxicology, College of Pharmacy, Chosun University, Gwangju 61452, Korea. 93_kjh@naver.com.RC-HCLD, College of Korean Medicine, Daegu Haany University, Gyeongsan, Gyeongsangbuk-do 38610, Korea. gucci200@hanmail.net.RC-HCLD, College of Korean Medicine, Daegu Haany University, Gyeongsan, Gyeongsangbuk-do 38610, Korea. skek023@dhu.ac.kr.Department of Biochemical and Polymer Engineering, Chosun University, Gwangju 61452, Korea. shinhj@chosun.ac.kr.Laboratory of Toxicology, College of Pharmacy, Chosun University, Gwangju 61452, Korea. shki@chosun.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30626086

Citation

Yang, Ji Hye, et al. "Bamboo Stems (Phyllostachys Nigra Variety Henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury." Nutrients, vol. 11, no. 1, 2019.
Yang JH, Choi MH, Na CS, et al. Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. Nutrients. 2019;11(1).
Yang, J. H., Choi, M. H., Na, C. S., Cho, S. S., Kim, J. H., Ku, S. K., Cho, I. J., Shin, H. J., & Ki, S. H. (2019). Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. Nutrients, 11(1). https://doi.org/10.3390/nu11010114
Yang JH, et al. Bamboo Stems (Phyllostachys Nigra Variety Henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. Nutrients. 2019 Jan 8;11(1) PubMed PMID: 30626086.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. AU - Yang,Ji Hye, AU - Choi,Moon-Hee, AU - Na,Chang-Su, AU - Cho,Sam Seok, AU - Kim,Jae Hoon, AU - Ku,Sae Kwang, AU - Cho,Il Je, AU - Shin,Hyun-Jae, AU - Ki,Sung Hwan, Y1 - 2019/01/08/ PY - 2018/11/27/received PY - 2018/12/28/revised PY - 2019/01/01/accepted PY - 2019/1/11/entrez PY - 2019/1/11/pubmed PY - 2019/3/21/medline KW - Nrf2 KW - PN3 KW - bamboo stems KW - iron overload KW - liver injury JF - Nutrients JO - Nutrients VL - 11 IS - 1 N2 - This study was designed to investigate the hepatoprotective effect of bamboo stems using in vitro and in vivo experimental liver damage models. Ethyl acetate fraction of 80% ethanol extract of Phyllostachys nigra stem (PN3) containing polyphenols had a higher NQO1-ARE reporter gene activity as monitored by the activity of the NF-E2-related factor (Nrf2) antioxidant pathway in cells in comparison to extracts from other species and under other conditions. The Nrf2 was translocated from the cytosol to the nucleus in response to PN3, followed by induction of the Nrf2 target gene expression, including HO-1, GCL, and NQO-1 in HepG2 cells. Phosphorylation of Nrf2 in HepG2 cells was enhanced in PN3, which was mediated by PKCδ, ERK, and p38 MAPK. Consequently, PN3 inhibited arachidonic acid (AA) + iron-induced reactive oxygen species generation and glutathione depletion, and, thus, highlighted their role in cytotoxicity. Treatment with major polyphenols of PN3, including catechin, chlorogenic acid, caffeic acid, and p-coumaric acid, also improved AA + iron-mediated oxidative stress and, thus, improved cell viability. Treatment with phenylhydrazine in mice, i.e., the iron overload liver injury model, increased plasma alanine aminotransferase and aspartate aminotransferase levels and changed histological features in mice-a response that was almost completely blocked by PN3 administration. Moreover, PN3 extract mitigated phenylhydrazine-induced oxidative stress and inflammatory responses. Conclusively, PN3 can exert a hepatoprotective effect against iron overload-induced acute liver damage due to its antioxidant properties. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/30626086/Bamboo_Stems__Phyllostachys_nigra_variety_henosis__Containing_Polyphenol_Mixtures_Activate_Nrf2_and_Attenuate_Phenylhydrazine_Induced_Oxidative_Stress_and_Liver_Injury_ L2 - http://www.mdpi.com/resolver?pii=nu11010114 DB - PRIME DP - Unbound Medicine ER -