Tags

Type your tag names separated by a space and hit enter

Molecular characterization of the insecticidal activity of double-stranded RNA targeting the smooth septate junction of western corn rootworm (Diabrotica virgifera virgifera).
PLoS One. 2019; 14(1):e0210491.Plos

Abstract

The western corn rootworm (WCR, Diabrotica virgifera virgifera) gene, dvssj1, is a putative homolog of the Drosophila melanogaster gene, snakeskin (ssk). This gene encodes a membrane protein associated with the smooth septate junction (SSJ) which is required for the proper barrier function of the epithelial lining of insect intestines. Disruption of DVSSJ integrity by RNAi technique has been shown previously to be an effective approach for corn rootworm control, by apparent suppression of production of DVSSJ1 protein leading to growth inhibition and mortality. To understand the mechanism that leads to the death of WCR larvae by dvssj1 double-stranded RNA, we examined the molecular characteristics associated with SSJ functions during larval development. Dvssj1 dsRNA diet feeding results in dose-dependent suppression of mRNA and protein; this impairs SSJ formation and barrier function of the midgut and results in larval mortality. These findings suggest that the malfunctioning of the SSJ complex in midgut triggered by dvssj1 silencing is the principal cause of WCR death. This study also illustrates that dvssj1 is a midgut-specific gene in WCR and its functions are consistent with biological functions described for ssk.

Links

PMC Free PDF

Authors+Show Affiliations

Hu X
DuPont Pioneer, Johnston, Iowa, United States of America.
Steimel JP
DuPont Pioneer, Johnston, Iowa, United States of America.
Kapka-Kitzman DM
DuPont Pioneer, Johnston, Iowa, United States of America.
Davis-Vogel C
DuPont Pioneer, Johnston, Iowa, United States of America.
Richtman NM
DuPont Pioneer, Johnston, Iowa, United States of America.
Mathis JP
DuPont Pioneer, Johnston, Iowa, United States of America.
Nelson ME
DuPont Pioneer, Johnston, Iowa, United States of America.
Lu AL
DuPont Pioneer, Johnston, Iowa, United States of America.
Wu G
DuPont Pioneer, Hayward, California, United States of America.

MeSH

AnimalsColeopteraDrosophila ProteinsDrosophila melanogasterGenes, InsectInsect ControlInsect ProteinsInsecticidesLarvaMembrane ProteinsPest Control, BiologicalRNA InterferenceRNA, Double-StrandedRNA, MessengerZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30629687

Citation

Hu, Xu, et al. "Molecular Characterization of the Insecticidal Activity of Double-stranded RNA Targeting the Smooth Septate Junction of Western Corn Rootworm (Diabrotica Virgifera Virgifera)." PloS One, vol. 14, no. 1, 2019, pp. e0210491.
Hu X, Steimel JP, Kapka-Kitzman DM, et al. Molecular characterization of the insecticidal activity of double-stranded RNA targeting the smooth septate junction of western corn rootworm (Diabrotica virgifera virgifera). PLoS One. 2019;14(1):e0210491.
Hu, X., Steimel, J. P., Kapka-Kitzman, D. M., Davis-Vogel, C., Richtman, N. M., Mathis, J. P., Nelson, M. E., Lu, A. L., & Wu, G. (2019). Molecular characterization of the insecticidal activity of double-stranded RNA targeting the smooth septate junction of western corn rootworm (Diabrotica virgifera virgifera). PloS One, 14(1), e0210491. https://doi.org/10.1371/journal.pone.0210491
Hu X, et al. Molecular Characterization of the Insecticidal Activity of Double-stranded RNA Targeting the Smooth Septate Junction of Western Corn Rootworm (Diabrotica Virgifera Virgifera). PLoS One. 2019;14(1):e0210491. PubMed PMID: 30629687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of the insecticidal activity of double-stranded RNA targeting the smooth septate junction of western corn rootworm (Diabrotica virgifera virgifera). AU - Hu,Xu, AU - Steimel,Joseph P, AU - Kapka-Kitzman,Deirdre M, AU - Davis-Vogel,Courtney, AU - Richtman,Nina M, AU - Mathis,John P, AU - Nelson,Mark E, AU - Lu,Albert L, AU - Wu,Gusui, Y1 - 2019/01/10/ PY - 2018/07/09/received PY - 2018/12/24/accepted PY - 2019/1/11/entrez PY - 2019/1/11/pubmed PY - 2019/10/23/medline SP - e0210491 EP - e0210491 JF - PloS one JO - PLoS One VL - 14 IS - 1 N2 - The western corn rootworm (WCR, Diabrotica virgifera virgifera) gene, dvssj1, is a putative homolog of the Drosophila melanogaster gene, snakeskin (ssk). This gene encodes a membrane protein associated with the smooth septate junction (SSJ) which is required for the proper barrier function of the epithelial lining of insect intestines. Disruption of DVSSJ integrity by RNAi technique has been shown previously to be an effective approach for corn rootworm control, by apparent suppression of production of DVSSJ1 protein leading to growth inhibition and mortality. To understand the mechanism that leads to the death of WCR larvae by dvssj1 double-stranded RNA, we examined the molecular characteristics associated with SSJ functions during larval development. Dvssj1 dsRNA diet feeding results in dose-dependent suppression of mRNA and protein; this impairs SSJ formation and barrier function of the midgut and results in larval mortality. These findings suggest that the malfunctioning of the SSJ complex in midgut triggered by dvssj1 silencing is the principal cause of WCR death. This study also illustrates that dvssj1 is a midgut-specific gene in WCR and its functions are consistent with biological functions described for ssk. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30629687/Molecular_characterization_of_the_insecticidal_activity_of_double_stranded_RNA_targeting_the_smooth_septate_junction_of_western_corn_rootworm__Diabrotica_virgifera_virgifera__ DB - PRIME DP - Unbound Medicine ER -