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Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR.
Indian J Pediatr. 2019 05; 86(5):433-438.IJ

Abstract

OBJECTIVE

To determine the frequency of respiratory pathogens in infants diagnosed with acute lower respiratory tract infections.

METHODS

A prospective cross-sectional observational study was conducted in infants hospitalized with a diagnosis of acute lower respiratory tract infection (ALRTI), in a tertiary care hospital in a metropolitan city of Western India. Nasopharyngeal swabs were analyzed by multiplex real time polymerase chain reaction, for 18 viruses and 3 bacteria (H. influenzae type b, C. pneumoniae and M. pneumoniae). The entire data was entered in Microsoft excel sheet and frequencies were determined.

RESULTS

One hundred eligible infants were enrolled. Pathogens were detected in 82 samples, which included Respiratory syncytial viruses (RSV) A / B (35.4%), Human rhinovirus (25.6%), Adenovirus (22%), Human Parainfluenza viruses (11%), Human bocavirus (9.8), Human metapneumovirus A / B (8.5%), Influenza A (H1N1) pdm 09 (6.1%), Parechovirus (3.7%), Human coronaviruses (3.66%), Haemophilus influenzae type b (6.1%), Chlamydia pneumoniae (2.4%) and Mycoplasma pneumoniae (2.4%). Influenza A (other than H1N1), Influenza B, Human Coronavirus 229E and Enterovirus were not detected. The rate of coinfection was 34% and rhinovirus was the most common of the multiple pathogens.

CONCLUSIONS

Spectrum of viral etiologies of ALRTI is highlighted. Etiological diagnosis of ALRTI would enable specific antiviral therapy, restrict antibiotic use and help in knowing burden of disease.

Authors+Show Affiliations

Department of Microbiology, T.N. Medical College & B.Y.L. Nair Hospital, Mumbai, Maharashtra, 400008, India. dr.anujasonawane@gmail.com.Department of Microbiology, T.N. Medical College & B.Y.L. Nair Hospital, Mumbai, Maharashtra, 400008, India.Department of Pediatrics, T.N. Medical College & B.Y.L. Nair Hospital, Mumbai, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30637585

Citation

Sonawane, Anuja A., et al. "Respiratory Pathogens in Infants Diagnosed With Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR." Indian Journal of Pediatrics, vol. 86, no. 5, 2019, pp. 433-438.
Sonawane AA, Shastri J, Bavdekar SB. Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR. Indian J Pediatr. 2019;86(5):433-438.
Sonawane, A. A., Shastri, J., & Bavdekar, S. B. (2019). Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR. Indian Journal of Pediatrics, 86(5), 433-438. https://doi.org/10.1007/s12098-018-2840-8
Sonawane AA, Shastri J, Bavdekar SB. Respiratory Pathogens in Infants Diagnosed With Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR. Indian J Pediatr. 2019;86(5):433-438. PubMed PMID: 30637585.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR. AU - Sonawane,Anuja A, AU - Shastri,Jayanthi, AU - Bavdekar,Sandeep B, Y1 - 2019/01/14/ PY - 2018/05/08/received PY - 2018/12/11/accepted PY - 2019/1/15/pubmed PY - 2020/1/28/medline PY - 2019/1/15/entrez KW - Acute lower respiratory tract infection KW - Co-infection KW - Infants KW - Multiplex real time PCR KW - RSV KW - Respiratory viruses SP - 433 EP - 438 JF - Indian journal of pediatrics JO - Indian J Pediatr VL - 86 IS - 5 N2 - OBJECTIVE: To determine the frequency of respiratory pathogens in infants diagnosed with acute lower respiratory tract infections. METHODS: A prospective cross-sectional observational study was conducted in infants hospitalized with a diagnosis of acute lower respiratory tract infection (ALRTI), in a tertiary care hospital in a metropolitan city of Western India. Nasopharyngeal swabs were analyzed by multiplex real time polymerase chain reaction, for 18 viruses and 3 bacteria (H. influenzae type b, C. pneumoniae and M. pneumoniae). The entire data was entered in Microsoft excel sheet and frequencies were determined. RESULTS: One hundred eligible infants were enrolled. Pathogens were detected in 82 samples, which included Respiratory syncytial viruses (RSV) A / B (35.4%), Human rhinovirus (25.6%), Adenovirus (22%), Human Parainfluenza viruses (11%), Human bocavirus (9.8), Human metapneumovirus A / B (8.5%), Influenza A (H1N1) pdm 09 (6.1%), Parechovirus (3.7%), Human coronaviruses (3.66%), Haemophilus influenzae type b (6.1%), Chlamydia pneumoniae (2.4%) and Mycoplasma pneumoniae (2.4%). Influenza A (other than H1N1), Influenza B, Human Coronavirus 229E and Enterovirus were not detected. The rate of coinfection was 34% and rhinovirus was the most common of the multiple pathogens. CONCLUSIONS: Spectrum of viral etiologies of ALRTI is highlighted. Etiological diagnosis of ALRTI would enable specific antiviral therapy, restrict antibiotic use and help in knowing burden of disease. SN - 0973-7693 UR - https://www.unboundmedicine.com/medline/citation/30637585/Respiratory_Pathogens_in_Infants_Diagnosed_with_Acute_Lower_Respiratory_Tract_Infection_in_a_Tertiary_Care_Hospital_of_Western_India_Using_Multiplex_Real_Time_PCR_ L2 - https://dx.doi.org/10.1007/s12098-018-2840-8 DB - PRIME DP - Unbound Medicine ER -